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Volume 12
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10.3390/diagnostics12040963
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Systematic Review

The Need for Standardization in Next-Generation Sequencing Studies for Classic Hodgkin Lymphoma: A Systematic Review

by
Antonio Santisteban-Espejo
1,2,3,
Irene Bernal-Florindo
2,*,
Jose Perez-Requena
1,
Lidia Atienza-Cuevas
1,
Julia Moran-Sanchez
3,4,
María del Carmen Fernandez-Valle
4,
Raquel Romero-Garcia
2 and
Marcial Garcia-Rojo
1,2
1
Department of Pathology, Puerta del Mar University Hospital, 11009 Cadiz, Spain
2
Institute of Research and Innovation in Biomedical Sciences of the Province of Cadiz (INiBICA), 11009 Cadiz, Spain
3
Department of Medicine, Faculty of Medicine, University of Cadiz, 11003 Cadiz, Spain
4
Department of Hematology and Hemotherapy, Puerta del Mar University Hospital, 11009 Cadiz, Spain
*
Author to whom correspondence should be addressed.
Diagnostics 2022, 12(4), 963; https://doi.org/10.3390/diagnostics12040963
Submission received: 14 March 2022 / Revised: 7 April 2022 / Accepted: 11 April 2022 / Published: 12 April 2022
(This article belongs to the Special Issue Advances in Lymphoma Molecular Diagnostics)
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Abstract

Classic Hodgkin lymphoma (cHL) constitutes a B cell-derived neoplasm defined by a scarce tumoral population, termed Hodgkin and Reed–Sternberg (HRS) cells, submerged into a histologically heterogeneous microenvironment. The paucity of HRS cells has historically hampered genetic studies, rendering the identification of the recurrent genetic lesions and molecular pathways deregulated in this lymphoma difficult. The advent of high-throughput sequencing methods such as next-generation sequencing (NGS) could sensibly optimize the identification of the mutational landscape of cHL. However, there is no current consensus either in the design of panels for targeted NGS or in its most relevant clinical applications. In this work, we systematically review the current state of NGS studies of cHL, stressing the need for standardization both in the candidate genes to be analyzed and the bioinformatic pipelines. As different institutions have developed and implemented their own customized NGS-based protocols, to compare and systematically review the major findings of this ongoing research area could be of added value for centers that routinely perform diagnostic, monitoring and genotyping strategies in cHL samples. The results of this systematic review should contribute to the interdepartmental harmonization and achievement of a consensus in the current clinical applications of NGS studies of cHL.
Keywords: Classic Hodgkin lymphoma; standardization; next-generation sequencing; liquid biopsy Classic Hodgkin lymphoma; standardization; next-generation sequencing; liquid biopsy

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MDPI and ACS Style

Santisteban-Espejo, A.; Bernal-Florindo, I.; Perez-Requena, J.; Atienza-Cuevas, L.; Moran-Sanchez, J.; Fernandez-Valle, M.d.C.; Romero-Garcia, R.; Garcia-Rojo, M. The Need for Standardization in Next-Generation Sequencing Studies for Classic Hodgkin Lymphoma: A Systematic Review. Diagnostics 2022, 12, 963. https://doi.org/10.3390/diagnostics12040963

AMA Style

Santisteban-Espejo A, Bernal-Florindo I, Perez-Requena J, Atienza-Cuevas L, Moran-Sanchez J, Fernandez-Valle MdC, Romero-Garcia R, Garcia-Rojo M. The Need for Standardization in Next-Generation Sequencing Studies for Classic Hodgkin Lymphoma: A Systematic Review. Diagnostics. 2022; 12(4):963. https://doi.org/10.3390/diagnostics12040963

Chicago/Turabian Style

Santisteban-Espejo, Antonio, Irene Bernal-Florindo, Jose Perez-Requena, Lidia Atienza-Cuevas, Julia Moran-Sanchez, María del Carmen Fernandez-Valle, Raquel Romero-Garcia, and Marcial Garcia-Rojo. 2022. "The Need for Standardization in Next-Generation Sequencing Studies for Classic Hodgkin Lymphoma: A Systematic Review" Diagnostics 12, no. 4: 963. https://doi.org/10.3390/diagnostics12040963

APA Style

Santisteban-Espejo, A., Bernal-Florindo, I., Perez-Requena, J., Atienza-Cuevas, L., Moran-Sanchez, J., Fernandez-Valle, M. d. C., Romero-Garcia, R., & Garcia-Rojo, M. (2022). The Need for Standardization in Next-Generation Sequencing Studies for Classic Hodgkin Lymphoma: A Systematic Review. Diagnostics, 12(4), 963. https://doi.org/10.3390/diagnostics12040963

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