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Article

Multiplex Immunofluorescence Assay of Infiltrating Mononu-Clear Cell Subsets in Acute T-Cell-Mediated Rejection and BK Virus-Associated Nephropathy in the Allograft Kidney

1
Department of Pathology, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu 41944, Korea
2
Division of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu 41944, Korea
3
Department of Convergence Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul 05505, Korea
4
Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Mauro Giuseppe Mastropasqua
Diagnostics 2022, 12(2), 268; https://doi.org/10.3390/diagnostics12020268
Received: 28 December 2021 / Revised: 15 January 2022 / Accepted: 19 January 2022 / Published: 21 January 2022
(This article belongs to the Section Optical Diagnostics)
Renal allograft biopsy is the gold standard procedure for diagnosis of kidney rejection via specific pathological changes. To provide a better assessment of immunologic events in acute T-cell-mediated rejection (acute TCMR) and BK virus-associated nephropathy (BKVAN) cases, we used multiplex immunofluorescence staining to identify infiltrating mononuclear cell subsets in the cortex area of transplanted kidneys. Antibodies to CD4, CD8, CD20, CD68, Foxp3, and cytokeratin were used. In cortical interstitium, CD8+ cells were significantly more prevalent in acute TCMR than BKVAN cases (34% vs. 22.8%, p = 0.034). In medulla, CD20+ cells were significantly more prevalent in BKVAN than acute TCMR cases (51.9% vs. 11.3%, p = 0.028). View Full-Text
Keywords: multiplex immunofluorescence assay; image analysis; T-cell-mediated rejection; BK virus-associated nephropathy; transplant kidney multiplex immunofluorescence assay; image analysis; T-cell-mediated rejection; BK virus-associated nephropathy; transplant kidney
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MDPI and ACS Style

Kim, M.-S.; Lim, J.-H.; Han, M.-H.; Kim, S.-Y.; Kim, Y.J.; Kim, Y.-J. Multiplex Immunofluorescence Assay of Infiltrating Mononu-Clear Cell Subsets in Acute T-Cell-Mediated Rejection and BK Virus-Associated Nephropathy in the Allograft Kidney. Diagnostics 2022, 12, 268. https://doi.org/10.3390/diagnostics12020268

AMA Style

Kim M-S, Lim J-H, Han M-H, Kim S-Y, Kim YJ, Kim Y-J. Multiplex Immunofluorescence Assay of Infiltrating Mononu-Clear Cell Subsets in Acute T-Cell-Mediated Rejection and BK Virus-Associated Nephropathy in the Allograft Kidney. Diagnostics. 2022; 12(2):268. https://doi.org/10.3390/diagnostics12020268

Chicago/Turabian Style

Kim, Mee-Seon, Jeong-Hoon Lim, Man-Hoon Han, Sang-Yeob Kim, Yun J. Kim, and Yong-Jin Kim. 2022. "Multiplex Immunofluorescence Assay of Infiltrating Mononu-Clear Cell Subsets in Acute T-Cell-Mediated Rejection and BK Virus-Associated Nephropathy in the Allograft Kidney" Diagnostics 12, no. 2: 268. https://doi.org/10.3390/diagnostics12020268

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