Cloning, Expression and Evaluation of Thioredoxin Peroxidase-1 Antigen for the Serological Diagnosis of Schistosoma mekongi Human Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Human Serum Samples
2.2. TPx-1 Sequence
2.3. Recombinant Protein Preparation
2.4. Enzyme-Linked Immunosorbent Assay (ELISA)
2.5. Statistical Analysis
3. Results
3.1. Cloning and Sequencing of SmekTPx-1
3.2. Expression and Purification of Recombinant Antigens
3.3. ELISA
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Antigen | Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | K 1 |
---|---|---|---|---|---|
SmekTPx-1 | 89.3 (95% CI: 71.8–97.3) | 93.3 (95% CI: 77.9–99.2) | 92.6 (95% CI: 76.5–97.7) | 90.3 (95% CI: 76.1–96.5) | 0.82 |
SjTPx-1 | 71.4 (95% CI: 51.3–86.8) | 66.7 (95% CI: 47.2–82.7) | 66.7 (95% CI: 53.4–77.7) | 71.4 (95% CI: 56.9–82.6) | 0.38 |
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Wanlop, A.; Angeles, J.M.M.; Macalanda, A.M.C.; Kirinoki, M.; Ohari, Y.; Yajima, A.; Yamagishi, J.; Ona, K.A.L.; Kawazu, S.-i. Cloning, Expression and Evaluation of Thioredoxin Peroxidase-1 Antigen for the Serological Diagnosis of Schistosoma mekongi Human Infection. Diagnostics 2022, 12, 3077. https://doi.org/10.3390/diagnostics12123077
Wanlop A, Angeles JMM, Macalanda AMC, Kirinoki M, Ohari Y, Yajima A, Yamagishi J, Ona KAL, Kawazu S-i. Cloning, Expression and Evaluation of Thioredoxin Peroxidase-1 Antigen for the Serological Diagnosis of Schistosoma mekongi Human Infection. Diagnostics. 2022; 12(12):3077. https://doi.org/10.3390/diagnostics12123077
Chicago/Turabian StyleWanlop, Atcharaphan, Jose Ma. M. Angeles, Adrian Miki C. Macalanda, Masashi Kirinoki, Yuma Ohari, Aya Yajima, Junya Yamagishi, Kevin Austin L. Ona, and Shin-ichiro Kawazu. 2022. "Cloning, Expression and Evaluation of Thioredoxin Peroxidase-1 Antigen for the Serological Diagnosis of Schistosoma mekongi Human Infection" Diagnostics 12, no. 12: 3077. https://doi.org/10.3390/diagnostics12123077