Next Article in Journal
Inter/Intra-Observer Agreement in Video-Capsule Endoscopy: Are We Getting It All Wrong? A Systematic Review and Meta-Analysis
Previous Article in Journal
Intra-Individual Changes in Total Procollagen-Type 1 N-terminal Propeptide in a Korean Adult Population
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Diagnostics
Volume 12
Issue 10
10.3390/diagnostics12102398
diagnostics-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Case Report

Adenosquamous Carcinoma with the Acantholytic Feature in the Oral Cavity: A Case Report and Comprehensive Literature Review

by
Tatsuya Abé
1,*,
Manabu Yamazaki
1,
Satoshi Maruyama
2,
Nobuyuki Ikeda
3,
Yoshimasa Sumita
3,
Kei Tomihara
3 and
Jun-ichi Tanuma
1
1
Division of Oral Pathology, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8514, Japan
2
Oral Pathology Section, Department of Surgical Pathology, Niigata University Hospital, Niigata 951-8520, Japan
3
Oral and Maxillofacial Surgery, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8514, Japan
*
Author to whom correspondence should be addressed.
Diagnostics 2022, 12(10), 2398; https://doi.org/10.3390/diagnostics12102398
Submission received: 12 September 2022 / Revised: 24 September 2022 / Accepted: 29 September 2022 / Published: 2 October 2022
(This article belongs to the Section Pathology and Molecular Diagnostics)
Browse Figures
Versions Notes

Abstract

Adenosquamous carcinoma (ASC) is an aggressive subtype of squamous cell carcinoma (SCC). Due to its poor prognosis, a precise pathological diagnosis of ASC is essential but challenging because its pathological criteria are still unclear. Here, we present a rare case of oral ASC accompanied by acantholytic features. The tumor was raised in the mandibular gingiva and recurred locally approximately 13 months after the initial surgery with cervical lymph node metastasis. Pathological specimens of the primary lesion showed acantholysis in a large area of the SCC. Mucous cells, the characteristic finding indicating glandular differentiation, were imperceptible in the initial surgical specimen but increased in the locally recurrent and metastatic lymph node specimens. In a comprehensive literature review of oral ASC cases, the present case was the only case of ASC with acantholytic features. We reconfirmed that ASC has poor prognoses, such as low 5-year overall survival and disease-free survival, high locoregional recurrence, and high distant metastasis rates. A precise diagnosis of ASC is required for estimating prognosis and undergoing close follow-up, even if the adenocarcinomatous component is limited to a small area in the lesion.

1. Introduction

Adenosquamous carcinoma (ASC) in the oral cavity is a rare subtype of squamous cell carcinoma (SCC) [1]. ASC in the head and neck region was first recognized by Gerughty et al. in 1968 [2]. Their report defined ASC as a malignant neoplasm containing unequivocal adenocarcinoma and separate areas that were distinctly SCC [2]. The differentiation of ASC from conventional SCC is necessary because ASC has a poorer prognosis than conventional SCC. ASC can be found in various organs; however, its histopathogenesis in the head and neck region is not fully understood, and its histological diagnostic criteria have not been clearly stated [1].
On the other hand, acantholytic SCC (AcSCC) is also well-recognized as a subtype of SCC [1]. Histologically, AcSCC is characterized by loss of cohesion among neoplastic prickle cells [3]. Lever first described the AcSCC in 1947 [4]. After the first report of cutaneous cases of AcSCC, cases in non-sun-exposed areas, such as the aerodigestive tract, including the oral mucosa [5], have been reported. Morphologically, AcSCC can frequently mimic glandular structures by acantholysis; therefore, it is called adenoid SCC. Despite the closeness of the morphologies between ASC and AcSCC, ASC has a poorer prognosis than AcSCC [6]. Thus, the pathological distinction of ASC from AcSCC is important for estimating patient prognosis.
Here, we report a rare case of ASC with the acantholytic feature and local and regional recurrences and its immunohistochemical profile. Furthermore, a comprehensive review of ASC cases in the oral cavity was performed to clarify their biological behavior.

2. Case Presentation

2.1. Clinical History

A 77-year-old male patient complained of delayed healing after the extraction of the right second molar of the mandible. On his first visit to our hospital, an intraoral examination revealed a deep ulcer with induration, measuring 25 × 20 mm, in the right molar region of the mandible (Figure 1A). No palpable nodules were observed on either side of the neck. Computed tomography (CT) revealed a bone-destructive lesion that expanded from the right molar gingiva to the mandibular bone, measuring 30 × 22 × 31 mm. Magnetic resonance imaging (MRI) revealed that the lesion showed a high signal intensity on T2-weighted imaging. An incisional biopsy specimen was diagnosed as a squamous cell carcinoma of the gingiva. Clinical staging was evaluated as cT4aN0M0, according to the TNM classification, 8th edition of the Union for International Cancer Control (UICC) [7]. Resection of the right side of the mandible to the buccal mucosa and elective neck dissection (level I of the right neck) were performed. A histological examination of the resected specimen revealed a complete tumor resection. No lymph node metastasis was observed, and no postoperative chemoradiotherapy was administered.
A year after the first operation, an ulcerative mass with induration in the right buccal mucosa of the first operation area was identified (Figure 1B). As CT and MRI also suggested local recurrence of cancer, an incisional biopsy was performed, and a diagnosis of squamous cell carcinoma compatible with local recurrence was made approximately 13 months later from the first resection. Positron emission tomography-CT revealed a cervical lymph node metastasis. Surgical resection of the right buccal mucosa and radical neck dissection, and subsequent radiotherapy, were performed. A year and a half after resectioning the recurrent lesion and neck dissection, no local or regional recurrence or distant metastasis were detected.

2.2. Pathological Findings

From the macroscopic findings of the resected sample of the right sectional mandibulectomy, a solid and whitish, poorly demarcated lesion with ulceration of the gingiva expanded to the mandibular bone marrow and fatty tissue surrounding the mandible. Histopathological examination revealed that the lesion predominantly consisted of well-differentiated SCC (Figure 2A), which was associated with an intraepithelial lesion in the gingival mucosa that invaded the mandibular bone marrow and peri-mandibular soft tissues. However, the SCC component largely exhibited acantholysis (Figure 2B). Less than 1% of the tumor area showed a gland-like structure with central tumor cell dissociation (Figure 2C). The gland-like structure consists of basaloid tumor cells, which were characterized by scanty and basophilic cytoplasm and condensed nuclei, and intracytoplasmic-mucin-containing goblet-like tumor cells. There were no findings of vascular and perineural invasion in the specimen. All surgical margins were tumor-free. The pathological classification was evaluated as pT4a according to UICC [7].
The resected specimen of the right buccal mucosa at recurrence was macroscopically covered with smooth and flat mucosa. On gross cutting, a poorly demarcated whitish lesion was observed in the muscular layer of the buccal region and expanded to the subcutaneous tissue. Histologically, well-differentiated SCC was evident; however, most parts of SCC showed acantholysis (Figure 2D). The acantholytic area showed central necrosis (Figure 2E). In addition, the acantholytic area of SCC was continuous with the gland-like structure with low-columnar cells and mucin-containing cells, and it was limited to less than 5% of the tumor (Figure 2F) in the specimen of the first operation. In the metastatic lesion in the neck lymph node, the SCC component with acantholysis and the glandular components, including mucinous cells and intraluminal mucin, were accompanied (Figure 2G). The cellular attachment was lost among the eosinophilic prickle cancer cells (Figure 2H). A gland-like structure with mucin-containing cells was also observed in the metastatic region of the right neck lymph nodes (Figure 2I). Approximately 50% of the tumor tissue of the metastatic lesion consisted of a component that showed a gland-like structure with mucin-containing cells.
The mucin-containing cells (Figure 3A) showed intracytoplasmic positivity for Alcian blue staining (Figure 3B), indicating acidic mucopolysaccharide production. Immunohistochemically, the mucin-containing cells were positive for carcinoembryonic antigen (CEA) (Figure 3C) and MUC1; they were partly positive for CAM5.2 but not for cytokeratin (CK) 7. In the periphery of the gland-like structure, the tumor cells were also positive for p40 (Figure 3D) and CK5/6 weakly (Figure 3E), but mucin-containing cells were not. These results suggest that mucin-containing cells were associated with glandular differentiation. However, the cancer cells in the glandular components retained the expression of squamous-differentiation-related molecules. The immunopositivity of p53 was strong and diffuse in both parts of the SCC with acantholysis and glandular/mucinous components (Figure 3F). Furthermore, MAML2 break-apart fluorescence in situ hybridization (FISH) was negative for the present lesion (Figure 4). According to the histological evaluation, the histopathological diagnosis was confirmed as ASC with acantholytic features.

3. Discussion

Here, we report a case of oral ASC with acantholytic features. The tumor consisted of a predominant SCC component with acantholytic features and a small amount of a glandular component. ASC with acantholytic features is rare. To our knowledge, no previous reports on the oral cavity exist. ASC associated with acantholysis has been reported in the esophagus [8] and pancreas [9]. The separation of ASC from conventional SCC and AcSCC is considerably important to predict the patient’s prognosis, since ASC shows a poorer prognosis. Both immunohistochemistry for multiple markers for glandular differentiation and special staining to detect mucin are highly recommended for diagnosing ASC.
The histological criteria for ASC of the oral cavity remain unclear. In the WHO classification, the definition of ASC is stated in the section of the tumor of the hypopharynx, larynx, trachea, and para-pharyngeal space as “a malignant tumor that arises from the surface epithelium and shows both squamous and glandular differentiation” [1]. However, the criteria for the amount of glandular differentiation have not been clarified. In our case, the amount of glandular component was considerably small in the primary lesion (<5%); however, it increased in the recurrent and metastatic lesions (up to 50%). This finding suggests that the glandular component is critical to defining the biological behavior of ASC, and the pathological diagnosis of ASC should be considered despite the amount of glandular component.
Although immunohistochemistry can help identify glandular differentiation, it also has limitations. The immunohistochemical positivity of CK7 and CAM5.2 might also support glandular differentiation in various organs [10,11]. However, CK7 was negative, and CAM5.2 was positive only in a small number of cancer cells in the present case. The glandular components of reported cases of oral ASC were typically positive for CK7 [12,13,14,15,16,17,18,19,20,21]. The mucin-containing cells were CEA-positive and p40-negative, similar to the immunohistochemical results for ASC reported by Satomi et al. [15]. These results suggest that mucin-containing cells possess a glandular phenotype, and CK7 is not a definitive marker of ASC in the oral mucosa. In addition, cancer cells located in the periphery of glandular structures were positive for p40, which is a squamous differentiation marker [22]. Immunoreactivity suggested that the squamous phenotype remained on the cancer cells in the glandular structure. For immunohistochemical evaluation, multiple types of intermediate filaments should be examined. In addition, non-neoplastic mucinous cells that remain in the cancer nest due to glandular involvement of cancer cells should be ruled out carefully during the diagnostic process.
SCC with acantholytic features, known as AcSCC [1], should be distinguished from ASC. AcSCC showed histological mimicking of the glandular structure, leading to the mistaking of true glands [23]. Thus, AcSCC is known as adenoid SCC [4]. This histological similarity between ASC and AcSCC/adenoid SCC causes confusion in diagnosis. However, the diagnostic separation of ASC from AcSCC is important because ASC has a poorer prognosis than AcSCC [24]. Adenoid components in AcSCC morphologically mimic glandular differentiation and are negative for mucin [25,26]. Therefore, to differentiate ASC from AcSCC, the identification and detection of mucin are crucial.
Mucoepidermoid carcinoma (MEC) of the salivary glands, including mucosa-associated minor salivary glands, can be a problematic differential diagnosis in the present case. Histopathologically, MEC consists of cancer cells with squamous and mucinous differentiation and “intermediate cells”. In molecular analysis, 56–75% of MEC cases showed rearrangement of the MAML2 gene [27]. The present case showed biphasic differentiation of cancer cells as well as MEC. However, intraepithelial or dysplastic precursor lesions observed in the present case suggested a mucosal epithelial origin of the tumor. Furthermore, the lack of “intermediate cells” and the negative results of MAML2 break-apart FISH also supported the diagnosis of ASC.
From our comprehensive literature review of ASC arising in the oral cavity (Table 1), 50 cases, including the present case, have been reported [2,12,13,14,15,16,17,18,19,20,21,23,28,29,30,31,32,33,34,35,36,37], and the clinical information of the review is summarized in Table 2. The median age of the patients was 60 (range 22–97). The sex ratio was approximately 2:1 for male:female. These tendencies are almost equivalent to the conventional SCC [1]. In terms of the site, the tongue is the most frequent primary site. However, the characteristic point was that the second frequent site was the floor of the mouth. This anatomical feature may be related to the distribution of secretory glands associated with the mucosal squamous epithelium.
Our review of the literature reconfirms the aggressiveness of ASC. Locoregional recurrence, local recurrence, regional lymph node metastasis, regional recurrence, distant metastasis rates, and the rate of death of the disease were calculated as 62.8% (27/43 cases), 52.2% (12/23), 46.5% (20/43), 14.0% (6/43), 30.0% (9/30), and 31.1% (13/42), respectively. Similarly, Keelawat et al. [23] reported that 46.7% of patients had experienced local recurrences: 64.7% had positive cervical lymph nodes, 23.1% developed distant metastases, and 42.9% died of their disease at a mean follow-up period of 24.7 months in ASC of the aerodigestive tract, including the oral cavity. Conventional oral SCC also had lymph node metastasis in approximately 40% of patients [38].
However, conventional SCC of the oral cavity showed an overall recurrence rate of 15.5–34.4%, with 5.4–19.9% local and 8.5–14.6% regional [39,40]. In addition, the distant metastasis rate of conventional SCC of the oral cavity is estimated to be 3–9.6% [41,42,43]. In comparing ASC with conventional SCC, unfavorable events, such as locoregional recurrences and distant metastasis, were markedly more frequent in patients with ASC. In terms of the outcome data (Table 1), the 5-year and 3-year overall survival (OS) rates of ASC were 37.1% [95% confidence interval (CI): 15.4–59.0%] and 46.3% [95% CI: 25.1–65.2%], respectively, and the median OS interval was 36 months. The 5-year and 3-year disease-free survival (DFS) rates were 28.0% [95% CI: 10.8–48.3%], and the median DFS interval was 13 months. Schick et al. reported that the 3-year OS and DFS rates were 52% [95% CI: 28–75%] and 32% [95% CI: 11–54%], respectively, while the median OS and DFS intervals were 39 and 12 months, respectively [31]. Meanwhile, the 5-year OS and DFS rates of conventional SCC of the oral cavity were 56–66% and 71–79%, respectively [44,45,46]. Our secondary analysis of the literature data has limitations in the analytical accuracy, since not all the reports we reviewed clarified the starting points of follow-up intervals and the detailed information that would affect the outcome, such as the tumor sizes, tumor stages, or therapeutic modality. The survival of the cancer patient is associated not only with the results of surgical resection but also with the effects and side effects of adjuvant therapy, such as radiotherapy and chemotherapy. Furthermore, the effect of adjuvant therapy is associated with various factors [47] including the histological subtype. The difference in the biological response to the various therapy between ASC cells and conventional SCC cells has been totally undetermined. Thus, our comparisons between ASC and conventional SCC are not accurate. However, we found that the ASC of the oral cavity may have poorer OS and DFS compared to both ASC cases of the head and neck region other than the oral cavity and conventional SCC cases.
To our knowledge, the present case is the only case of ASC with acantholytic features arising in the oral mucosa among reviewed cases. Although detailed pathological findings were unavailable for most of the cases in the literature reviewed, mucinous cells were observed in only 10 cases. Cases without mucinous cells were diagnosed based on glandular structures. Lymphatic and vascular invasion was observed in only three cases. Perineural invasion was observed in 11 patients. Age, TNM category, and surgical treatment were independently associated with OS, advanced T stage, distant metastases, and surgery for disease-specific survival [48]. However, the histological factors affecting prognosis, such as the percentage of glandular differentiation, are unknown.
In conclusion, we report that ASC consists of a squamous component with acantholytic features and an adenocarcinoma component with mucinous cell differentiation. Because ASC is a malignant neoplasm with a poor prognosis, a precise and accurate histological diagnosis is essential to estimate a patient’s outcome. Intracytoplasmic mucin can be a clue for diagnosing ASC, and special staining or immunohistochemical analysis for multiple markers to confirm glandular or mucinous differentiation is recommended. Although the mucinous component is limited to only a small area of the primary lesion, we recommend describing it in the pathological report and close clinical follow-up.

Author Contributions

Conceptualization, T.A.; methodology, T.A.; validation, T.A., S.M., M.Y.; formal analysis, T.A.; resources, T.A., N.I., Y.S.; writing—original draft preparation, T.A.; writing—review and editing, T.A., S.M., M.Y., K.T., J.-i.T. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

This study was conducted in accordance with the fundamental principles of the latest revised Declaration of Helsinki.

Informed Consent Statement

Written informed consent was obtained from the patient involved in the study.

Data Availability Statement

Data sharing is not applicable to this article.

Acknowledgments

We would like to thank Takafumi Hayashi and Hideyoshi Nishiyama of the Division of Oral and Maxillofacial Radiology, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, for their helpful radiological diagnosis.

Conflicts of Interest

No competing financial interest exists.

References

  1. El-Naggar, A.K.; Chan, J.K.C.; Grandis, J.R.; Takata, T.; Slootweg, P.J. (Eds.) WHO Head and Neck Tumours Classification, 4th ed.; The International Agency for Research on Cancer: Lyon, France, 2017; pp. 89, 109–111. [Google Scholar]
  2. Gerughty, R.M.; Hennigar, G.R.; Brown, F.M. Adenosquamous carcinoma of the nasal, oral and laryngeal cavities. A clinicopathologic survey of ten cases. Cancer 1968, 22, 1140–1155. [Google Scholar] [CrossRef]
  3. Ogawa, T.; Kiuru, M.; Konia, T.H.; Fung, M.A. Acantholytic squamous cell carcinoma is usually associated with hair follicles, not acantholytic actinic keratosis, and is not “high risk”: Diagnosis, management, and clinical outcomes in a series of 115 cases. J. Am. Acad. Dermatol. 2017, 76, 327–333. [Google Scholar] [CrossRef] [PubMed]
  4. Lever, W.F. ADENOACANTHOMA OF SWEAT GLANDS: Carcinoma of Sweat Glands with Glandular and Epidermal Elements; Report of Four Cases. Arch. Derm. Syphilol. 1947, 56, 157–171. [Google Scholar] [CrossRef] [PubMed]
  5. Kerawala, C.J. Acantholytic squamous cell carcinoma of the oral cavity: A more aggressive entity? Br. J. Oral Maxillofac. Surg. 2009, 47, 123–125. [Google Scholar] [CrossRef] [PubMed]
  6. Kass, J.I.; Lee, S.C.; Abberbock, S.; Seethala, R.R.; Duvvuri, U. Adenosquamous carcinoma of the head and neck: Molecular analysis using CRTC-MAML FISH and survival comparison with paired conventional squamous cell carcinoma. Laryngoscope 2015, 125, E371–E376. [Google Scholar] [CrossRef] [PubMed]
  7. Brierley, J.D.; Gospodarowicz, M.K.; Wittekind, D. (Eds.) TNM Classification of Malignant Tumours, 8th ed.; Wiley Blackwell: Hoboken, NJ, USA, 2017; pp. 18–21. [Google Scholar]
  8. Matsukuma, S.; Takahashi, O.; Utsumi, Y.; Tsuda, M.; Miyai, K.; Okada, K.; Takeo, H. Esophageal adenosquamous carcinoma mimicking acantholytic squamous cell carcinoma. Oncol. Lett. 2017, 14, 4918–4922. [Google Scholar] [CrossRef][Green Version]
  9. Alwaheeb, S.; Chetty, R. Adenosquamous carcinoma of the pancreas with an acantholytic pattern together with osteoclast-like and pleomorphic giant cells. J. Clin. Pathol. 2005, 58, 987–990. [Google Scholar] [CrossRef]
  10. Gyure, K.A.; Morrison, A.L. Cytokeratin 7 and 20 Expression in Choroid Plexus Tumors: Utility in Differentiating These Neoplasms from Metastatic Carcinomas. Mod. Pathol. 2000, 13, 638–643. [Google Scholar] [CrossRef]
  11. He, S.; Peng, J.; Li, L.; Xu, Y.; Wu, X.; Yu, J.; Liu, J.; Zhang, J.; Zhang, R.; Wang, W. High expression of cytokeratin CAM5.2 in esophageal squamous cell carcinoma is associated with poor prognosis. Medicine 2019, 98, e17104. [Google Scholar] [CrossRef]
  12. Prabhakar, M.; Sabarinath, B.; Sivapathasundharam, B.; Vasanthakumar, V. Adenosquamous carcinoma of the tongue: A case report and an overview of histogenetic concepts. J. Oral Maxillofac. Pathol. 2020, 24 (Suppl. 1), S110–S114. [Google Scholar] [CrossRef]
  13. Eguchi, T.; Basugi, A.; Kanai, I.; Miyata, Y.; Suzuki, T.; Hamada, Y. Adenosquamous carcinoma development as a recurrence of squamous cell carcinoma in the oral floor: A case report. Medicine 2019, 98, e17688. [Google Scholar] [CrossRef] [PubMed]
  14. Kikuta, S.; Todoroki, K.; Seki, N.; Kusukawa, J. Adenosquamous Carcinoma in the Midline Dorsum of the Tongue: A Rare Case Report. J. Oral Maxillofac. Surg. 2018, 76, 2131–2135. [Google Scholar] [CrossRef]
  15. Satomi, T.; Kohno, M.; Hasagawa, O.; Enomoto, A.; Abukawa, H.; Chikazu, D.; Yoshida, M.; Matsubayashi, J.; Nagao, T. Adenosquamous carcinoma of the tongue: Clinicopathologic study and review of the literature. Odontology 2017, 105, 127–135. [Google Scholar] [CrossRef] [PubMed]
  16. Kusafuka, K.; Muramatsu, K.; Iida, Y.; Mori, K.; Miki, T.; Suda, T.; Fuke, T.; Kamijo, T.; Onitsuka, T.; Nakajima, T. MUC expression in adenosquamous carcinoma of the head and neck regions of Japanese patients: Immunohistochemical analysis. Pathol. Int. 2014, 64, 104–114. [Google Scholar] [CrossRef] [PubMed]
  17. Ishida, M.; Iwai, M.; Kagotani, A.; Iwamoto, N.; Okabe, H. Adenosquamous carcinoma of the tongue: A case report with review of the literature. Int. J. Clin. Exp. Pathol. 2014, 7, 1809–1813. [Google Scholar] [PubMed]
  18. Also, L.; Castillo, M.; Nadal, A.; Caballero, M.; Mallofre, C.; Palacin, A.; Cardesa, A. Adenosquamous carcinoma of the head and neck: Criteria for diagnosis in a study of 12 cases. Histopathology 2004, 44, 570–579. [Google Scholar] [CrossRef] [PubMed]
  19. Sheahan, P.; Fitzgibbon, J.; Lee, G.; O’Leary, G. Adenosquamous carcinoma of the tongue in a 22-year-old female: Report of a case with immunohistochemistry. Eur. Arch. Otorhinolaryngol. 2003, 260, 509–512. [Google Scholar] [CrossRef]
  20. Izumi, K.; Nakajima, T.; Maeda, T.; Cheng, J.; Saku, T. Adenosquamous carcinoma of the tongue: Report of a case with histochemical, immunohistochemical, and ultrastructural study and review of the literature. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 1998, 85, 178–184. [Google Scholar] [CrossRef]
  21. Curi, M.M.; de Oliveira, M.F.; Condezo, A.F.B.; Lima, H.G.; da Silva, A.A.; DeAntoni, C.C.; Cardoso, C.L. Adenosquamous Carcinoma Affecting the Maxilla: A Case Report and Review. J. Cancer Ther. 2017, 8, 286–295. [Google Scholar] [CrossRef][Green Version]
  22. Ma, Y.; Fan, M.; Dai, L.; Kang, X.; Liu, Y.; Sun, Y.; Xiong, H.; Liang, Z.; Yan, W.; Chen, K. Expression of p63 and CK5/6 in early-stage lung squamous cell carcinoma is not only an early diagnostic indicator but also correlates with a good prognosis. Thorac. Cancer 2015, 6, 288–295. [Google Scholar] [CrossRef]
  23. Keelawat, S.; Liu, C.Z.; Roehm, P.C.; Barnes, L. Adenosquamous carcinoma of the upper aerodigestive tract: A clinicopathologic study of 12 cases and review of the literature. Am. J. Otolaryngol. 2002, 23, 160–168. [Google Scholar] [CrossRef] [PubMed]
  24. Minić, A.J.; Stajčić, Z. Adenosquamous carcinoma of the inferior turbinate: A case report. J. Oral Maxillofac. Surg. 1994, 52, 764–767. [Google Scholar] [CrossRef]
  25. Terada, T. Adenoid squamous cell carcinoma of the oral cavity. Int. J. Clin. Exp. Pathol. 2012, 5, 442–447. [Google Scholar] [PubMed]
  26. Kusafuka, K.; Ebihara, M.; Ishiki, H.; Takizawa, Y.; Iida, Y.; Onitsuka, T.; Takakuwa, R.; Kasami, M.; Ito, I.; Kameya, T. Primary adenoid squamous cell carcinoma of the oral cavity. Pathol. Int. 2006, 56, 78–83. [Google Scholar] [CrossRef] [PubMed]
  27. Cipriani, N.A.; Lusardi, J.J.; McElherne, J.; Pearson, A.T.; Olivas, A.D.; Fitzpatrick, C.; Lingen, M.W.; Blair, E.A. Mucoepidermoid Carcinoma: A Comparison of Histologic Grading Systems and Relationship to MAML2 Rearrangement and Prognosis. Am. J. Surg. Pathol. 2019, 43, 885–897. [Google Scholar] [CrossRef] [PubMed]
  28. Rawal, Y.B.; Anderson, K.M. Adenosquamous Carcinoma of the Tongue. Head Neck Pathol. 2018, 12, 576–579. [Google Scholar] [CrossRef]
  29. Bhattacharyya, I.; Chehal, H.K.; McNally, S.J.; Cohen, D.M.; Islam, N.M. Adenosquamous carcinoma of the oral cavity: An unusual epithelial malignancy. A report of two cases and review of literature. J. Oral Maxillofac. Surg. Med. Pathol. 2015, 27, 126–130. [Google Scholar] [CrossRef]
  30. Thanakappan, P.; Venkata, N.; Amudala, R.; Botu, M. Adenosquamous carcinoma of oral cavity. J. Cancer Res. Ther. 2015, 11, 1034. [Google Scholar] [CrossRef]
  31. Schick, U.; Pusztaszeri, M.; Betz, M.; Ghadjar, P.; Demiroz, C.; Kaanders, J.H.A.M.; Ozsahin, M. Adenosquamous carcinoma of the head and neck: Report of 20 cases and review of the literature. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. 2013, 116, 313–320. [Google Scholar] [CrossRef]
  32. Masand, R.P.; El-Mofty, S.K.; Ma, X.J.; Luo, Y.; Flanagan, J.J.; Lewis, J.S. Adenosquamous Carcinoma of the Head and Neck: Relationship to Human Papillomavirus and Review of the Literature. Head Neck Pathol. 2011, 5, 108–116. [Google Scholar] [CrossRef]
  33. Kothari, R.K.; Ghosh, A.; Bhattacharyya, S.K.; Ghosh, S.K. Adenosquamous carcinoma of oral cavity: A case report. J. Indian Med. Assoc. 2007, 105, 531–532. [Google Scholar]
  34. Yoshimura, Y.; Mishima, K.; Obara, S.; Yoshimura, H.; Maruyama, R. Clinical characteristics of oral adenosquamous carcinoma: Report of a case and an analysis of the reported Japanese cases. Oral Oncol. 2003, 39, 309–315. [Google Scholar] [CrossRef]
  35. Scully, C.; Porter, S.R.; Speight, P.M.; Eveson, J.W.; Gale, D. Adenosquamous carcinoma of the mouth: A rare variant of squamous cell carcinoma. Int. J. Oral Maxillofac. Surg. 1999, 28, 125–128. [Google Scholar] [CrossRef]
  36. Abdelsayed, R.A.; Sangueza, O.P.; Newhouse, R.F.; Singh, B.S. Adenosquamous carcinoma: A case report with immunohistochemical evaluation. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endodontology 1998, 85, 173–177. [Google Scholar] [CrossRef]
  37. Napier, S.S.; Gormley, J.S.; Newlands, C.; Ramsay-Baggs, P. Adenosquamous carcinoma. A rare neoplasm with an aggressive course. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endodontology 1995, 79, 607–611. [Google Scholar] [CrossRef]
  38. Lea, J.; Bachar, G.; Sawka, A.M.; Lakra, D.C.; Gilbert, R.W.; Irish, J.C.; Brown, D.H.; Gullane, P.J.; Goldstein, D.P. Metastases to level IIb in squamous cell carcinoma of the oral cavity: A systematic review and meta-analysis. Head Neck 2010, 32, 184–190. [Google Scholar] [CrossRef] [PubMed]
  39. Ord, R.A.; Kolokythas, A.; Reynolds, M.A. Surgical Salvage for Local and Regional Recurrence in Oral Cancer. J. Oral Maxillofac. Surg. 2006, 64, 1409–1414. [Google Scholar] [CrossRef] [PubMed]
  40. Lee, D.Y.; Kang, S.H.; Kim, J.H.; Kim, M.S.; Oh, K.H.; Woo, J.S.; Kwon, S.Y.; Jung, K.Y.; Baek, S.K. Survival and recurrence of resectable tongue cancer: Resection margin cutoff value by T classification. Head Neck 2018, 40, 283–291. [Google Scholar] [CrossRef] [PubMed]
  41. Verschuur, H.P.; Irish, J.C.; O’Sullivan, B.; Goh, C.; Gullane, P.J.; Pintilie, M. A Matched Control Study of Treatment Outcome in Young Patients With Squamous Cell Carcinoma of the Head and Neck. Laryngoscope 1999, 109, 249–258. [Google Scholar] [CrossRef] [PubMed]
  42. Garavello, W.; Ciardo, A.; Spreafico, R.; Gaini, R.M. Risk Factors for Distant Metastases in Head and Neck Squamous Cell Carcinoma. Arch. Otolaryngol. Head Neck Surg. 2006, 132, 762. [Google Scholar] [CrossRef]
  43. González-García, R.; Naval-Gías, L.; Román-Romero, L.; Sastre-Pérez, J.; Rodríguez-Campo, F.J. Local recurrences and second primary tumors from squamous cell carcinoma of the oral cavity: A retrospective analytic study of 500 patients. Head Neck 2009, 31, 1168–1180. [Google Scholar] [CrossRef] [PubMed]
  44. Rogers, S.N.; Brown, J.S.; Woolgar, J.A.; Lowe, D.; Magennis, P.; Shaw, R.J.; Sutton, D.; Errington, D.; Vaughan, D. Survival following primary surgery for oral cancer. Oral Oncol. 2009, 45, 201–211. [Google Scholar] [CrossRef] [PubMed]
  45. Zanoni, D.K.; Montero, P.H.; Migliacci, J.C.; Shah, J.P.; Wong, R.J.; Ganly, I.; Patel, S.G. Survival outcomes after treatment of cancer of the oral cavity (1985–2015). Oral Oncol. 2019, 90, 115–121. [Google Scholar] [CrossRef]
  46. Faisal, M.; Dhanani, R.; Ullah, S.; Bakar, M.A.; Irfan, N.; Malik, K.I.; Loya, A.; Boban, E.M.; Hussain, R.; Jamshed, A. Prognostic outcomes of treatment naïve oral tongue squamous cell carcinoma (OTSCC): A comprehensive analysis of 14 years. Eur. Arch. Otorhinolaryngol. 2021, 278, 3045–3053. [Google Scholar] [CrossRef]
  47. De Felice, F.; Musio, D.; Terenzi, V.; Valentini, V.; Cassoni, A.; Tombolini, M.; de Vincentiis, M.; Tombolini, V. Treatment improvement and better patient care: Which is the most important one in oral cavity cancer? Radiat. Oncol. 2014, 9, 263. [Google Scholar] [CrossRef]
  48. Liu, Y.; Mi, Y.; Zhang, L.; Jiang, T. Survival and risk factors of adenosquamous carcinoma in the oral and maxillofacial region: A population-based study. Am. J. Transl. Res. 2021, 13, 12071–12082. [Google Scholar] [PubMed]
Diagnostics 12 02398 g001
Figure 1. Intraoral findings of adenosquamous carcinoma (ASC) with the acantholytic feature. At initial onset (A) and recurrence (B). After extracting the second molar of the right side of the mandible, an ulcerative lesion remained, and a large mass expanded to the buccal side of the first molar (A). At a recurrence, a submucosal mass with an ulcer was located in the right buccal mucosa (B).
Figure 1. Intraoral findings of adenosquamous carcinoma (ASC) with the acantholytic feature. At initial onset (A) and recurrence (B). After extracting the second molar of the right side of the mandible, an ulcerative lesion remained, and a large mass expanded to the buccal side of the first molar (A). At a recurrence, a submucosal mass with an ulcer was located in the right buccal mucosa (B).
Diagnostics 12 02398 g001
Diagnostics 12 02398 g002
Figure 2. Histology of ASC with the acantholytic feature in the primary (A–C), recurrent (D–F), and metastatic (G–I) lesions. Hematoxylin–eosin (HE) staining (A–I). Arrows, mucin-containing cells. Bars, 250 μm (A,D,G), 100 μm (B,E,H), and 50 μm (C,F,I). In the primary mandibulectomy specimen, a small glandular/mucinous component was formed in the cancer tissue within the well-differentiated squamous cell carcinoma (SCC) component (A). The SCC component shows acantholysis in the glandular/mucinous component (C), small cuboidal cells lining the luminal structure, and a small number of cuboidal cells with intracytoplasmic basophilic mucin (arrows). Multinucleated foreign body-type giant cells were present in the luminal space. In the resected buccal mucosa at recurrence, acantholysis of the SCC component is observed in a large part of the SCC (E). Less than 5% of cancer cells and the mandibulectomy sections showed glandular or mucinous differentiation. In the glandular component, small basophilic cuboidal cells showed a polarized nuclear location and prominent nucleoli intermingled with goblet-like cells with intracytoplasmic mucin (F). The components of SCC with acantholysis and glandular/mucinous components were admixed, even in lymph nodes with cancer metastasis (H). The glandular/mucinous component was observed in larger areas of the metastatic lesions to the cervical lymph nodes than in the primary site (E). The metastasized carcinoma showed an obvious glandular structure with intraluminal mucin lined by cuboidal cells and mucin-containing cells (I).
Figure 2. Histology of ASC with the acantholytic feature in the primary (A–C), recurrent (D–F), and metastatic (G–I) lesions. Hematoxylin–eosin (HE) staining (A–I). Arrows, mucin-containing cells. Bars, 250 μm (A,D,G), 100 μm (B,E,H), and 50 μm (C,F,I). In the primary mandibulectomy specimen, a small glandular/mucinous component was formed in the cancer tissue within the well-differentiated squamous cell carcinoma (SCC) component (A). The SCC component shows acantholysis in the glandular/mucinous component (C), small cuboidal cells lining the luminal structure, and a small number of cuboidal cells with intracytoplasmic basophilic mucin (arrows). Multinucleated foreign body-type giant cells were present in the luminal space. In the resected buccal mucosa at recurrence, acantholysis of the SCC component is observed in a large part of the SCC (E). Less than 5% of cancer cells and the mandibulectomy sections showed glandular or mucinous differentiation. In the glandular component, small basophilic cuboidal cells showed a polarized nuclear location and prominent nucleoli intermingled with goblet-like cells with intracytoplasmic mucin (F). The components of SCC with acantholysis and glandular/mucinous components were admixed, even in lymph nodes with cancer metastasis (H). The glandular/mucinous component was observed in larger areas of the metastatic lesions to the cervical lymph nodes than in the primary site (E). The metastasized carcinoma showed an obvious glandular structure with intraluminal mucin lined by cuboidal cells and mucin-containing cells (I).
Diagnostics 12 02398 g002
Diagnostics 12 02398 g003
Figure 3. Immunohistochemistry and mucin staining profiles of ASC with the acantholytic feature. HE staining (A), Alcian blue (B), carcinoembryonic antigen (CEA) (C), p40 (D), cytokeratin (CK) 5/6 (E), and p53 (F). Bars, 100 μm (A–F) and 50 μm (inset). A representative image of ASC in metastasized lymph nodes shows that the glandular component with intraluminal mucin deposition transitioned to the squamoid component with acantholysis (A). Alcian blue-positive acidic mucin was observed in the cytoplasm of the goblet-shaped cells and intraluminal mucin (B). CEA was strongly positive in the cytoplasm of the cancer cells, consisting predominantly of glandular structures, but also in the dissociated cancer cells in the squamoid component (C). Diffuse p40 nuclear positivity was observed in the squamoid and glandular cells, except for mucin-containing cells (D). CK5/6 was faintly positive, predominantly in the squamoid component (E). Diffuse strong p53 positivity was observed in both components (F).
Figure 3. Immunohistochemistry and mucin staining profiles of ASC with the acantholytic feature. HE staining (A), Alcian blue (B), carcinoembryonic antigen (CEA) (C), p40 (D), cytokeratin (CK) 5/6 (E), and p53 (F). Bars, 100 μm (A–F) and 50 μm (inset). A representative image of ASC in metastasized lymph nodes shows that the glandular component with intraluminal mucin deposition transitioned to the squamoid component with acantholysis (A). Alcian blue-positive acidic mucin was observed in the cytoplasm of the goblet-shaped cells and intraluminal mucin (B). CEA was strongly positive in the cytoplasm of the cancer cells, consisting predominantly of glandular structures, but also in the dissociated cancer cells in the squamoid component (C). Diffuse p40 nuclear positivity was observed in the squamoid and glandular cells, except for mucin-containing cells (D). CK5/6 was faintly positive, predominantly in the squamoid component (E). Diffuse strong p53 positivity was observed in both components (F).
Diagnostics 12 02398 g003
Diagnostics 12 02398 g004
Figure 4. Fluorescent in situ hybridization with MAML2 break-apart probes of the present case. Bar, 20 μm. In the present case, the tumor cells showed no split signal of MAML2 probes (overlapped red and green signals). A triple overlap signal in the individual nuclei was frequently observed, which might indicate aneuploidy.
Figure 4. Fluorescent in situ hybridization with MAML2 break-apart probes of the present case. Bar, 20 μm. In the present case, the tumor cells showed no split signal of MAML2 probes (overlapped red and green signals). A triple overlap signal in the individual nuclei was frequently observed, which might indicate aneuploidy.
Diagnostics 12 02398 g004
Table 1. A comprehensive review of cases of adenosquamous cell carcinoma in the oral cavity.
Table 1. A comprehensive review of cases of adenosquamous cell carcinoma in the oral cavity.
AgeSexSiteIntraoral FindingsHistological Feature of Adenocarcinoma ComponentLymphatic/Vascular/Perineural InvasionTreatmentLN Metastasis
at the Initial Operation		Local Recurrence (Months)Regional
LN Recurrence (Months)		Distant Metastasis
(Months)		Outcome (Months)Reference
77Mlower gingivaindurative ulcerglandular, mucus cell, acantholyticLy−, V+, Pn−SO → SO, ND, RTNE+13+13− AWOD31the present case
50Mtonguean ulceroproliferative lesion with indurationglandularNANANANA NA NA NA [12]
70Mfloor of the mouth a painful nodule with indurationglandularNANAC → SO → SO, ND, CRTNE+9+9−NADOD14[13]
48Mtonguea painless massglandularNASONE− − − AWOD9[14]
51Ftonguea painful ulcerglandular, mucous cellLy+, Pn+NANANA NA NA NA [28]
65Mtonguewell-defined indurated ulcer, with a leukoplakic lesionglandular, mucous cellLy+, V+, Pn+SO, ND, CRT+− NA +6AWOD30[15]
47Fmaxillanodular lesionglandular, mucinPn+SO, ND, RT → SO → SO++4 & 8− −72AWOD72[21]
97Fmaxillaulcerglandular, mucous cellNASONE− − − AWOD12[29]
59Flower gingivaulcerglandular, pseudo-ductalNASO → CRTNE+12NA NA DOD23[29]
55Mupper gingivaswellingductalNANANANA NA NA NA [30]
62MtongueNANANASONE− NA NA AWOD17[16]
59FtongueNAglandNASO → ND+NA NA NA DOD11[16]
63MtongueNANANASO, ND?−− NA NA AWOD32[16]
82MtongueNANANASO, ND+NA NA NA DOD6[16]
55FtongueNAglandNASO, ND → CT−− NA NA AWOD17[16]
61MtongueNANANASO, ND−− NA NA AWOD19[16]
83MtongueNANANASO, ND+− NA NA AWOD22[16]
71Ftonguereddish tumor
with hemorrhage and erosion		glandular, mucous cellLy+, V−SONE− − − AWOD1[17]
71NAfloor of the mouthNANANASO, RTNA+NA+NA+ (lung, bone)NADOD7[31]
60NAfloor of the mouthNANANASO, RTNA+NA−NA+ (lung)NADOD9[31]
68NAtongueNANANASO, RTNA− − − AWOD10[31]
60NAfloor of the mouthNANANASO, RCTNA− − − AWOD62[31]
42MtongueNANANANA−NA NA + (mediastinum)NADWD34[32]
65FtongueNANANANA−NA NA − NANA[32]
48MtongueNANANANA+− NA − AWOD3.5[32]
87FtongueNANANANA+NA NA − AWD9[32]
62Msoft palate/tonsilNANANANA+NA NA + (neck skin)NADWD36[32]
70Mupper and lower gingivaswellingglandularNARTNE (clinically +)NA NA +at the initialNA [33]
42MmouthNAductal, mucous cellNANANA− NA NA AWD20[18]
54MmouthNAductalNANANANA NA NA AWD36[18]
68MtongueNAductal, mucous cellNANANA− NA NA AWOD56[18]
43MmouthNAductal, mucous cellNANANANA NA NA DOD12[18]
72Mfloor of the mouthulcerative elevationductalNASO, ND+− − − DWOD60[34]
22FtongueulcerductalPn+SO, ND → SO−+9NA NA DWD12[19]
38Mfloor of the mouthNANAPn+SO, ND, RT+NA NA − NA [23]
80Mfloor of the mouthNANAPn+SO, RT → NA++12NA − AWOD47[23]
59Ffloor of the mouthulcerNAPn+SO, ND, RT++9+NA− AWD31[23]
74Mfloor of the mouthulcerNAPn+SO, RTNE+12NA − DWD24[23]
34Fpalateplate massNAPn−SO, RT → NANE− NA − AWOD78[23]
67Mlower gingivaulcer with raised edgeductalPn+RTNE− − − NANA[35]
46Mfloor of the mouthindurated, ulcerated massglandular, mucous cellNASO, partial ND−− − − AWOD6[35]
65Ftongueindurated ulcerductal structureV+, Pn+SONE− − − AWOD4[35]
78Ftonguediffuse reddish swellingglandularNACRTNE (clinically +)NA NA NE DOD4[20]
42Mtonguepainful ulcer, erythroplakiaductalPn+SO, ND−NA NA NA NA [36]
61Fupper gingivaswellingglandular, mucous cellNASO, RT+ (FNA)NA − NA DOD6.5[37]
54MtongueNANANASO, CTNA (Non-regional +?)NA NA +NADOD9[2]
53MtongueNANANASO, NDNA (Non-regional +?)NA NA +NADOD180[2]
60MtongueNANANASO, NDNA (Non-regional +?)NA NA +NADOD12[2]
47Mfloor of the mouthNANANASO → SO, ND++36+36− DOD36[2]
57Mfloor of the mouthNANANASO → SO, ND++3+3− AWD8[2]
Abbreviations: M, male; F, female; Ly, lymphatic invasion; V, vascular invasion; Pn, perineural invasion; SO, surgical operation; ND, neck dissection; CRT, chemoradiotherapy; RT, radiotherapy; CT, chemotherapy; FNA, fine-needle aspiration; LN, lymph node; AWD, alive with disease; AWOD, alive without disease; DOD, dead of disease; DWD, dead with disease; DWOD, dead without disease; NE, not evaluated; NA, not available; +, positive; −, negative.
Table 2. Summary of the literature review.
Table 2. Summary of the literature review.
Age 22–97 (Median 60)
Sexmale3162.0%
female1530.0%
unknown48.0%
Sitetongue2549.0%
floor of the mouth1019.6%
upper gingiva59.8%
lower gingiva47.8%
palate23.9%
not specified59.8%
    Locoregional recurrence27/4362.8%
    Local recurrence12/2352.2%
    Lymph node metastasis20/4346.5%
at initial operation17/4339.5%
regional lymph node recurrence6/4314.0%
    Distant metastasis9/3030.0%
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Abé, T.; Yamazaki, M.; Maruyama, S.; Ikeda, N.; Sumita, Y.; Tomihara, K.; Tanuma, J.-i. Adenosquamous Carcinoma with the Acantholytic Feature in the Oral Cavity: A Case Report and Comprehensive Literature Review. Diagnostics 2022, 12, 2398. https://doi.org/10.3390/diagnostics12102398

AMA Style

Abé T, Yamazaki M, Maruyama S, Ikeda N, Sumita Y, Tomihara K, Tanuma J-i. Adenosquamous Carcinoma with the Acantholytic Feature in the Oral Cavity: A Case Report and Comprehensive Literature Review. Diagnostics. 2022; 12(10):2398. https://doi.org/10.3390/diagnostics12102398

Chicago/Turabian Style

Abé, Tatsuya, Manabu Yamazaki, Satoshi Maruyama, Nobuyuki Ikeda, Yoshimasa Sumita, Kei Tomihara, and Jun-ichi Tanuma. 2022. "Adenosquamous Carcinoma with the Acantholytic Feature in the Oral Cavity: A Case Report and Comprehensive Literature Review" Diagnostics 12, no. 10: 2398. https://doi.org/10.3390/diagnostics12102398

APA Style

Abé, T., Yamazaki, M., Maruyama, S., Ikeda, N., Sumita, Y., Tomihara, K., & Tanuma, J.-i. (2022). Adenosquamous Carcinoma with the Acantholytic Feature in the Oral Cavity: A Case Report and Comprehensive Literature Review. Diagnostics, 12(10), 2398. https://doi.org/10.3390/diagnostics12102398

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop