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Article

Evaluation of 16S rDNA Heart Tissue PCR as a Complement to Blood Cultures for the Routine Etiological Diagnosis of Infective Endocarditis

by
Raquel Rodríguez-García
1,2,3,†,
María Ángeles Rodríguez-Esteban
1,
Jonathan Fernández-Suárez
4,
Ana Morilla
4,
Enrique García-Carús
5,
Mauricio Telenti
4,
Carlos Morales
6,
Guillermo Muñiz Albaiceta
1,2,3,7 and
Javier Fernández
2,4,*,†
1
Unidad de Cuidados Intensivos Cardiológicos, Hospital Universitario Central de Asturias, 33011 Oviedo, Spain
2
Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain
3
CIBER-Enfermedades Respiratorias, Instituto de Salud Carlos III, 28220 Madrid, Spain
4
Servicio de Microbiología Clínica, Hospital Universitario Central de Asturias, 33011 Oviedo, Spain
5
Servicio de Medicina Interna, Hospital Universitario Central de Asturias, 33011 Oviedo, Spain
6
Servicio de Cirugía Cardiaca, Hospital Universitario Central de Asturias, 33011 Oviedo, Spain
7
Departamento de Biología Funcional, Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, 33003 Oviedo, Spain
*
Author to whom correspondence should be addressed.
†
Same contribution.
Diagnostics 2021, 11(8), 1372; https://doi.org/10.3390/diagnostics11081372
Submission received: 29 June 2021 / Revised: 18 July 2021 / Accepted: 27 July 2021 / Published: 30 July 2021
(This article belongs to the Special Issue Diagnostic Infectious Disease and Microbiology)
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Abstract

Identification of the causative pathogen is required to optimize the effective therapy in infective endocarditis (IE). The aim of this study was to assess a 16S rDNA PCR to identify bacteria from heart valve tissues and to evaluate its usefulness as a complement to blood and removed valves cultures. A total of 266 patients diagnosed with IE from January 2015 to December 2019 were evaluated. Results between 16S rDNA PCR from heart valve tissues were compared with microbiological cultures. Blood cultures were positive in 83.5% of patients diagnosed with IE, while 39.6% and 71.8% of the evaluated heart valve samples were positive by culture and 16S rDNA PCR, respectively. For 32 (12%) patients, 16S rDNA tissue PCR provided valuable information supporting the results of blood cultures in the case of bacteria characteristic from the skin microbiota. Additionally, a microorganism was identified by using 16S rDNA PCR in 36% of blood culture-negative cases. The present study reveals that molecular diagnosis using 16S rDNA tissue PCR provides complementary information for the diagnosis of IE, and it should be recommended in surgical endocarditis, especially when blood cultures are negative.
Keywords: infective endocarditis; 16S rDNA PCR; blood culture; valve heart culture; molecular diagnosis infective endocarditis; 16S rDNA PCR; blood culture; valve heart culture; molecular diagnosis

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MDPI and ACS Style

Rodríguez-García, R.; Rodríguez-Esteban, M.Á.; Fernández-Suárez, J.; Morilla, A.; García-Carús, E.; Telenti, M.; Morales, C.; Albaiceta, G.M.; Fernández, J. Evaluation of 16S rDNA Heart Tissue PCR as a Complement to Blood Cultures for the Routine Etiological Diagnosis of Infective Endocarditis. Diagnostics 2021, 11, 1372. https://doi.org/10.3390/diagnostics11081372

AMA Style

Rodríguez-García R, Rodríguez-Esteban MÁ, Fernández-Suárez J, Morilla A, García-Carús E, Telenti M, Morales C, Albaiceta GM, Fernández J. Evaluation of 16S rDNA Heart Tissue PCR as a Complement to Blood Cultures for the Routine Etiological Diagnosis of Infective Endocarditis. Diagnostics. 2021; 11(8):1372. https://doi.org/10.3390/diagnostics11081372

Chicago/Turabian Style

Rodríguez-García, Raquel, María Ángeles Rodríguez-Esteban, Jonathan Fernández-Suárez, Ana Morilla, Enrique García-Carús, Mauricio Telenti, Carlos Morales, Guillermo Muñiz Albaiceta, and Javier Fernández. 2021. "Evaluation of 16S rDNA Heart Tissue PCR as a Complement to Blood Cultures for the Routine Etiological Diagnosis of Infective Endocarditis" Diagnostics 11, no. 8: 1372. https://doi.org/10.3390/diagnostics11081372

APA Style

Rodríguez-García, R., Rodríguez-Esteban, M. Á., Fernández-Suárez, J., Morilla, A., García-Carús, E., Telenti, M., Morales, C., Albaiceta, G. M., & Fernández, J. (2021). Evaluation of 16S rDNA Heart Tissue PCR as a Complement to Blood Cultures for the Routine Etiological Diagnosis of Infective Endocarditis. Diagnostics, 11(8), 1372. https://doi.org/10.3390/diagnostics11081372

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