Next Article in Journal
Laboratory Diagnosis of Paratyphoid Fever: Opportunity of Surface Plasmon Resonance
Previous Article in Journal
Overexpression of EIF5A2 Predicts Poor Prognosis in Patients with Oral Squamous Cell Carcinoma
Previous Article in Special Issue
Current Update of Laboratory Molecular Diagnostics Advancement in Management of Colorectal Cancer (CRC)
Open AccessArticle

Value of Serum NEUROG1 Methylation for the Detection of Advanced Adenomas and Colorectal Cancer

1
Department of Biochemistry, Genetics and Immunology, University of Vigo, 36310 Vigo, Spain
2
Centro de Investigaciones Biomédicas, Centro Singular de Investigación de Galicia, University of Vigo, 36310 Vigo, Spain
3
Department of Gastroenterology, Complexo Hospitalario Universitario de Ourense, Instituto de Investigación Biomédica Galicia Sur, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), 32005 Ourense, Spain
4
Department of Gastroenterology, Xerencia de Xestión Integrada de Vigo, Instituto de Investigación Biomédica Galicia Sur, 36213 Vigo, Spain
5
Department of Clinical Analysis, Complexo Hospitalario Universitario de Ourense, Instituto de Investigación Biomédica Galicia Sur, 32005 Ourense, Spain
*
Author to whom correspondence should be addressed.
Diagnostics 2020, 10(7), 437; https://doi.org/10.3390/diagnostics10070437
Received: 27 May 2020 / Revised: 23 June 2020 / Accepted: 26 June 2020 / Published: 28 June 2020
Aberrant DNA methylation detected in liquid biopsies is a promising approach for colorectal cancer (CRC) detection, including premalignant advanced adenomas (AA). We evaluated the diagnostic capability of serum NEUROG1 methylation for the detection of AA and CRC. A CpG island in NEUROG1 promoter was assessed by bisulfite pyrosequencing in a case-control cohort to select optimal CpGs. Selected sites were evaluated through a nested methylation-specific qPCR custom assay in a screening cohort of 504 asymptomatic family-risk individuals. Individuals with no colorectal findings and benign pathologies showed low serum NEUROG1 methylation, similar to non-advanced adenomas. Contrarily, individuals bearing AA or CRC (advanced neoplasia—AN), exhibited increased NEUROG1 methylation. Using >1.3518% as NEUROG1 cut-off (90.60% specificity), 33.33% of AN and 32.08% of AA were identified, detecting 50% CRC cases. Nonetheless, the combination of NEUROG1 with fecal immunochemical test (FIT), together with age and gender through a multivariate logistic regression resulted in an AUC = 0.810 for AN, and 0.796 for AA, detecting all cancer cases and 35–47% AA (specificity 98–95%). The combination of NEUROG1 methylation with FIT, age and gender demonstrated a convenient performance for the detection of CRC and AA, providing a valuable tool for CRC screening programs in asymptomatic individuals. View Full-Text
Keywords: DNA methylation; NEUROG1; colorectal cancer; advanced adenomas; screening; serum biomarker; FIT DNA methylation; NEUROG1; colorectal cancer; advanced adenomas; screening; serum biomarker; FIT
Show Figures

Figure 1

MDPI and ACS Style

Otero-Estévez, O.; Gallardo-Gomez, M.; Páez de la Cadena, M.; Rodríguez-Berrocal, F.J.; Cubiella, J.; Hernandez Ramirez, V.; García-Nimo, L.; De Chiara, L. Value of Serum NEUROG1 Methylation for the Detection of Advanced Adenomas and Colorectal Cancer. Diagnostics 2020, 10, 437.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop