Characterization of Stem-like Circulating Tumor Cells in Pancreatic Cancer
Abstract
:1. Introduction
2. Results
2.1. Pancreatic CTCs Can Be Clustered into Stem-Like and Non-Stem like Categories
2.2. Both Epithelial and Mesenchymal Markers are Expressed on CTC-S
2.3. The Adherens Junction Pathway is Functionally Enriched in CTC-S
2.4. Ctnnb1 and Klf4 Positively Correlate
2.5. CTNNB1 Is a Negative Prognostic Factor in Human PDAC
3. Discussion
4. Materials and Methods
4.1. Data Collection, Data Quality Check, and Normalization
4.2. Principal Component Analysis (PCA)
4.3. Identification of Differentially Expressed Genes
4.4. Weighted Gene Co-Expression Network Analysis (WGCNA)
4.5. Definition of Hub Genes
4.6. Functional Enrichment Analysis
4.7. Kaplan-Meier Survival Plot
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Zhu, L.; Hissa, B.; Győrffy, B.; Jann, J.-C.; Yang, C.; Reissfelder, C.; Schölch, S. Characterization of Stem-like Circulating Tumor Cells in Pancreatic Cancer. Diagnostics 2020, 10, 305. https://doi.org/10.3390/diagnostics10050305
Zhu L, Hissa B, Győrffy B, Jann J-C, Yang C, Reissfelder C, Schölch S. Characterization of Stem-like Circulating Tumor Cells in Pancreatic Cancer. Diagnostics. 2020; 10(5):305. https://doi.org/10.3390/diagnostics10050305
Chicago/Turabian StyleZhu, Lei, Barbara Hissa, Balázs Győrffy, Johann-Christoph Jann, Cui Yang, Christoph Reissfelder, and Sebastian Schölch. 2020. "Characterization of Stem-like Circulating Tumor Cells in Pancreatic Cancer" Diagnostics 10, no. 5: 305. https://doi.org/10.3390/diagnostics10050305