Combining Bioinformatics and Experiments to Identify CREB1 as a Key Regulator in Senescent Granulosa Cells
Abstract
:1. Introduction
2. Results
2.1. Predicted Function and Pathway Enrichment Analysis
2.2. Establishment and Characteristics of the Aged Granulosa Cells
2.3. Mitochondrial Dysfunction in Granulosa Cells Undergoing Senescence
2.4. Abnormal Mitochondrial Dynamics in Aged Granulosa Cells
2.5. Bioinformatic Analysis of CREB1 Function in Ovary
2.6. Regulation of the CREB/PRKAA Transcription Factor Axis in Aged Granulosa Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Treatment
4.2. Cell Proliferation Assay
4.3. RT-PCR
4.4. Mitochondrial Functional Analysis (ROS and MMP)
4.5. Fluorescent Labeling
4.6. Big Data Analytics Tools
4.7. Telomerase Activity Assay
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Lin, P.-H.; Lin, L.-T.; Li, C.-J.; Kao, P.-G.; Tsai, H.-W.; Chen, S.-N.; Wen, Z.-H.; Wang, P.-H.; Tsui, K.-H. Combining Bioinformatics and Experiments to Identify CREB1 as a Key Regulator in Senescent Granulosa Cells. Diagnostics 2020, 10, 295. https://doi.org/10.3390/diagnostics10050295
Lin P-H, Lin L-T, Li C-J, Kao P-G, Tsai H-W, Chen S-N, Wen Z-H, Wang P-H, Tsui K-H. Combining Bioinformatics and Experiments to Identify CREB1 as a Key Regulator in Senescent Granulosa Cells. Diagnostics. 2020; 10(5):295. https://doi.org/10.3390/diagnostics10050295
Chicago/Turabian StyleLin, Pei-Hsuan, Li-Te Lin, Chia-Jung Li, Pei-Gang Kao, Hsiao-Wen Tsai, San-Nung Chen, Zhi-Hong Wen, Peng-Hui Wang, and Kuan-Hao Tsui. 2020. "Combining Bioinformatics and Experiments to Identify CREB1 as a Key Regulator in Senescent Granulosa Cells" Diagnostics 10, no. 5: 295. https://doi.org/10.3390/diagnostics10050295