Next Article in Journal
Efficacy and Safety of Non-Anesthesiologist Administration of Propofol Sedation in Endoscopic Ultrasound: A Propensity Score Analysis
Next Article in Special Issue
Limited Diagnostic Utility of Chromogranin A Measurements in Workup of Neuroendocrine Tumors
Previous Article in Journal
Evaluation of Impact of Factors Affecting CT Radiation Dose for Optimizing Patient Dose Levels
Previous Article in Special Issue
Multifunctional Roles of miR-34a in Cancer: A Review with the Emphasis on Head and Neck Squamous Cell Carcinoma and Thyroid Cancer with Clinical Implications
Open AccessArticle

64Cu-DOTATATE Positron Emission Tomography (PET) of Borrelia Burgdorferi Infection: In Vivo Imaging of Macrophages in Experimental Model of Lyme Arthritis

1
Department of Clinical Physiology, Nuclear Medicine & PET, Rigshospitalet, DK-2100 Copenhagen, Denmark
2
Cluster for Molecular Imaging, Department of Biomedical Sciences, University of Copenhagen, DK-2200 Copenhagen, Denmark
3
Department of Infectious Diseases, Copenhagen University Hospital, Rigshospitalet, DK-2100 Copenhagen, Denmark
4
Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, DK-2200 Copenhagen, Denmark
5
Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, DK-2100 Copenhagen, Denmark
6
Center for Rheumatology and Spine Diseases, Institute for Inflammation Research, Copenhagen University Hospital, Rigshospitalet, DK-2100, Copenhagen, Denmark
7
Institute of Clinical Medicine, University of Copenhagen, DK-2100 Copenhagen, Denmark
*
Author to whom correspondence should be addressed.
Diagnostics 2020, 10(10), 790; https://doi.org/10.3390/diagnostics10100790
Received: 18 September 2020 / Revised: 29 September 2020 / Accepted: 2 October 2020 / Published: 6 October 2020
(This article belongs to the Collection Feature Papers)
Macrophages play a key role in the inflammatory response in Lyme arthritis (LA) and could be a target for diagnosing and monitoring active Borrelia burgdorferi sensu lato (Bb) infection. Therefore, we evaluated the potential of macrophage imaging using 64Cu-DOTATATE PET/CT for detection of Bb activity in a murine model of LA. LA was established in C3H/HeNRj mice infected with Bb B31 strain ML23 pBBE22luc. Bioluminescence imaging was performed to detect migration of spirochetes and inflammatory phagocytes to the joints. Three weeks post-infection 64Cu-DOTATATE PET/CT imaging was performed at an early (3 h) and late (48 h) time point. Plasma levels of a systemic macrophage marker in plasma CD163 were measured. 64Cu-DOTATATE uptake in infected joints was increased at the early (p < 0.0001) and late time points (p = 0.0005) compared with uptake in non-infected controls. No significant difference in plasma levels of CD163 was measured. 64Cu-DOTATATE PET allows for in vivo detection and quantification of LA locally in the joints through non-invasive visualization of macrophages. In contrast, measurement of a systemic macrophage marker in plasma, CD163, did not allow to detect disease. We suggest that 64Cu-DOTATATE PET could become a valuable diagnostic tool for in situ detection of Bb infection-related inflammation. View Full-Text
Keywords: lyme borreliosis; borrelia burgdorferi sensu lato complex; animal model; macrophage; positron emission tomography (PET); 64Cu-DOTATATE PET lyme borreliosis; borrelia burgdorferi sensu lato complex; animal model; macrophage; positron emission tomography (PET); 64Cu-DOTATATE PET
Show Figures

Graphical abstract

MDPI and ACS Style

Skovsbo Clausen, A.; Ørbæk, M.; Renee Pedersen, R.; Oestrup Jensen, P.; Lebech, A.-M.; Kjaer, A. 64Cu-DOTATATE Positron Emission Tomography (PET) of Borrelia Burgdorferi Infection: In Vivo Imaging of Macrophages in Experimental Model of Lyme Arthritis. Diagnostics 2020, 10, 790. https://doi.org/10.3390/diagnostics10100790

AMA Style

Skovsbo Clausen A, Ørbæk M, Renee Pedersen R, Oestrup Jensen P, Lebech A-M, Kjaer A. 64Cu-DOTATATE Positron Emission Tomography (PET) of Borrelia Burgdorferi Infection: In Vivo Imaging of Macrophages in Experimental Model of Lyme Arthritis. Diagnostics. 2020; 10(10):790. https://doi.org/10.3390/diagnostics10100790

Chicago/Turabian Style

Skovsbo Clausen, Anne; Ørbæk, Mathilde; Renee Pedersen, Regitze; Oestrup Jensen, Peter; Lebech, Anne-Mette; Kjaer, Andreas. 2020. "64Cu-DOTATATE Positron Emission Tomography (PET) of Borrelia Burgdorferi Infection: In Vivo Imaging of Macrophages in Experimental Model of Lyme Arthritis" Diagnostics 10, no. 10: 790. https://doi.org/10.3390/diagnostics10100790

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop