Diagnosis of Hodgkin Lymphoma from Cell Block: A Reliable and Helpful Tool in “Selected” Diagnostic Practice
Abstract
:1. Introduction
2. Materials and Methods
2.1. US-FNA Procedure
2.2. EBUS-FNA Procedure
2.3. Drainage Procedure
2.4. Complications of Procedures
2.5. CC Procedure
2.6. CB Procedure
2.7. Immunocytochemical Analysis
2.8. FISH Procedure
3. Results
3.1. Cytological Samples and Disease Location
3.2. Cytological Diagnosis and Ancillary Studies
3.3. Statistical Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Disclosure
Conflicts of Interest
References
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Case Number | Sex | Age at Diagnosis | Procedure | Site | Method | Clinical Indications | Clinical Queries | Cytological Diagnosis | ICC | FISH |
---|---|---|---|---|---|---|---|---|---|---|
1 | M | 75 | Drainage | PLE | CB | Pleural effusion | Carcinoma | LLC/SLL | Yes | |
2 | F | 19 | US-FNA | MM | CB | Follow-up in HL | Carcinoma | Strongly suggestive for HL | Yes | |
3 | M | 79 | US-FNA | LN | CB + Sm | Soft tissue mass | Lymphoma | DLBCL, ABC type | Yes | |
4 | M | 62 | US-FNA | ST | Sm | Follow-up in NHL | Lymphoma | Compatible with NHL B | Inadequate | |
5 | F | 78 | Drainage | PE | Sm | Retroperitoneal mass | Carcinoma | Compatible with NHL B | Inadequate | |
6 | M | 74 | US-FNA | SP | Sm | MD | Lymphoma | Compatible with NHL B | Inadequate | |
7 | M | 78 | Drainage | PLE | Cc | NHL | Lymphoma | Compatible with NHL B | Inadequate | |
8 | M | 45 | US-FNA | SP | Sm | Suspicious lymphoma | Lymphoma | Compatible with NHL B | Inadequate | |
9 | M | 33 | US-FNA | ST | Sm | MM | Lymphoma | Compatible with lymphoma | Inadequate | |
10 | M | 52 | US-FNA | SP | Sm | Suspicious HCL | Lymphoma | Compatible with NHL B | Inadequate | |
11 | F | 80 | US-FNA | SP | Sm | Splenic mass | Lymphoma | Compatible with lymphoma | Inadequate | |
12 | F | 29 | US-FNA | Lung | Sm | Lung consolidation | Lobar pneumonia | Compatible with lymphoma | Inadequate | |
13 | F | 89 | US-FNA | ST | Sm | Localized lymphadenopathy | Carcinoma | Compatible with NHL B | Inadequate | |
14 | F | 48 | Drainage | PLE | Cc | Follow-up in NHL | Lymphoma | Compatible with NHL T | Yes | |
15 | F | 55 | US-FNA | ST | Sm | Lymphadenomegaly | NQ | Inadequate | Inadequate | |
16 | F | 70 | US-FNA | LN | CB + Sm | Follow-up in NHL | Lymphoma | Suspicious for lymphoma | Yes | |
17 | F | 83 | US-FNA | MM | CB | Follow-up in NHL | Lymphoma | DLBCL, GC type | Yes | |
18 | M | 82 | US-FNA | LN | CB + Sm | Retroperitoneal mass | Lymphoma | NHL B, FL | Yes | |
19 | F | 68 | EBUS-FNA | MM | CB | Suspicious carcinoma | Carcinoma | HL | Yes | |
20 | M | 74 | US-FNA | ST | CB | Suspicious carcinoma | Carcinoma | MCL | Yes | Yes |
21 | M | 74 | Drainage | PLE | CB | Suspicious carcinoma | Carcinoma | DLBCL, ABC type | Yes | Yes |
22 | M | 74 | US-FNA | Liver | CB | Suspicious carcinoma | Carcinoma | DLBCL, ABC type | Yes | |
23 | F | 70 | US-FNA | ST | CB | Bone and pulmonary lesion | Carcinoma | Plasmocytoma | Yes | |
24 | F | 18 | US-FNA | Lung | CB | Cystic pulmonary lesion | TB | HL | Yes | |
25 | M | 41 | EBUS-FNA | MM | CB | Penile carcinoma and MM | Carcinoma | HL | Yes | |
26 | F | 65 | US-FNA | MM | CB | Mediastinic mass | Carcinoma | Suspicious for lymphoma | Inadequate | |
27 | M | 72 | US-FNA | MM | CB + Sm | Suspicious lymphoma | Lymphoma | DLBCL, ABC type | Yes | |
28 | M | 26 | US-FNA | LN | Sm | Lymphadenomegaly | Lymphoma | Suspicious for lymphoma | Inadequate | |
29 | F | 54 | US-FNA | LN | CB + Sm | Follow-up in NHL | Lymphoma | DLBCL, GC type | Yes | |
30 | M | 30 | US-FNA | MM | CB + Sm | Mediastinic mass | Thymoma/lymphoma | Strongly suggestive for HL | Yes | |
31 | M | 78 | Drainage | PE | Cc | Suspicious lymphoma | Lymphoma | Compatible with NHL | Inadequate | |
32 | M | 42 | US-FNA | Liver | CB + Sm | Suspicious lymphoma | Lymphoma | DLBCL, NOS | Yes | |
33 | F | 75 | Drainage | PE | CB + Cc | Peritoneal effusion | Lymphoma | DLBCL, NOS | Yes | Yes |
34 | F | 57 | US-FNA | LN | CB | Lymphadenomegaly | Carcinoma | DLBCL, GC type | Yes | |
35 | F | 88 | Drainage | PLE | Sm | Follow-up in HL | Lymphoma | Inadequate | Inadequate | |
36 | F | 77 | US-FNA | ST | CB | Abdominal mass | Lymphoma | DLBCL, GC type | Yes | |
37 | F | 93 | US-FNA | ST | CB + Sm | Soft tissue mass | Carcinoma | DLBCL, NOS | Yes | |
38 | M | 73 | EBUS-FNA | LN | CB | Pulmonary mass | Carcinoma | Suspicious for lymphoma | Inadequate | |
39 | M | 35 | US-FNA | ST | CB + Sm | Acute leukemia | Carcinoma | Inadequate | Inadequate | |
40 | F | 55 | US-FNA | MM | CB + Sm | Suspicious lymphoma | Lymphoma | Inadequate | Inadequate | |
41 | F | 55 | US-FNA | MM | Sm | Suspicious lymphoma | Lymphoma | Suspicious for lymphoma | Inadequate | |
42 | F | 67 | US-FNA | LN | Sm | Suspicious lymphoma | Lymphoma | Suspicious for lymphoma | Inadequate | |
43 | F | 40 | US-FNA | LN | Sm | Suspicious lymphoma | Lymphoma | Inadequate | Inadequate | |
44 | F | 48 | US-FNA | LN | Sm | Suspicious lymphoma | Lymphoma | Inadequate | Inadequate | |
45 | F | 76 | US-FNA | MM | CB + Sm | Suspicious lymphoma | Lymphoma | Inadequate | Inadequate | |
46 | M | 64 | US-FNA | LN | Sm | Suspicious lymphoma | Lymphoma | Inadequate | Inadequate | |
47 | M | 68 | US-FNA | ST | CB | Follow-up in NHL-mantle cell | Lymphoma | MCL | Yes | Yes |
Results | CCs | CBs | p-Value | Test Used and Result | ||
---|---|---|---|---|---|---|
n = 20 | n = 27 | |||||
n | % | n | % | |||
Inadequate | 5 | 25 | 3 | 11 | p = 0.394 | df2 overall (Fisher Exact Test) |
Compatibile | 12 | 60 | 21 | 78 | p = 0.187 | df1 Compatibile vs. Inadeguate&Suspicious (Pearson Chi-square Test) |
Suspicious | 3 | 15 | 3 | 11 | p = 0.258 | df1 Inadeguate vs. Compatibile&Suspicious (Fisher Exact Test) |
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Parente, P.; Covelli, C.; Zanelli, M.; Trombetta, D.; Carosi, I.; Carbonelli, C.; Sperandeo, M.; Mastracci, L.; Biancofiore, G.; Zizzo, M.; et al. Diagnosis of Hodgkin Lymphoma from Cell Block: A Reliable and Helpful Tool in “Selected” Diagnostic Practice. Diagnostics 2020, 10, 748. https://doi.org/10.3390/diagnostics10100748
Parente P, Covelli C, Zanelli M, Trombetta D, Carosi I, Carbonelli C, Sperandeo M, Mastracci L, Biancofiore G, Zizzo M, et al. Diagnosis of Hodgkin Lymphoma from Cell Block: A Reliable and Helpful Tool in “Selected” Diagnostic Practice. Diagnostics. 2020; 10(10):748. https://doi.org/10.3390/diagnostics10100748
Chicago/Turabian StyleParente, Paola, Claudia Covelli, Magda Zanelli, Domenico Trombetta, Illuminato Carosi, Cristiano Carbonelli, Marco Sperandeo, Luca Mastracci, Giovanni Biancofiore, Maurizio Zizzo, and et al. 2020. "Diagnosis of Hodgkin Lymphoma from Cell Block: A Reliable and Helpful Tool in “Selected” Diagnostic Practice" Diagnostics 10, no. 10: 748. https://doi.org/10.3390/diagnostics10100748