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Volume 16
Issue 6
10.3390/life16060994
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Environmental Application of Newly Designed Loop-Mediated Isothermal Amplification (LAMP) Kits for Nosocomial Pathogen Detection in Hospital Settings

by
Federica Marino
,
Caterina Bonincontro
,
Laura Caligaris
,
Carlo Derelitto
,
Luna Girolamini
and
Sandra Cristino
*
Department of Biological, Geological, and Environmental Sciences, University of Bologna, 40126 Bologna, Italy
*
Author to whom correspondence should be addressed.
Life 2026, 16(6), 994; https://doi.org/10.3390/life16060994 (registering DOI)
Submission received: 9 April 2026 / Revised: 6 June 2026 / Accepted: 9 June 2026 / Published: 12 June 2026
(This article belongs to the Section Microbiology)
Versions Notes

Abstract

Nosocomial pathogens persist on hospital surfaces contributing to healthcare-associated infections (HAIs), especially among vulnerable patients and in the presence of multidrug-resistant strains. Environmental surveillance is essential to prevent cross-contamination and support timely infection control interventions. However, conventional culture-based methods, although considered the gold standard, are labor-intensive and time-consuming, often delaying critical responses. This study evaluated loop-mediated isothermal amplification (LAMP) as a rapid screening tool for hospital environmental monitoring. A total of 100 surface samples were collected from different hospital wards and analyzed using both culture and LAMP assays targeting six major HAI-related pathogens: Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus spp., Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii. LAMP showed excellent performance, with sensitivity of 1.00 for all targets and specificity ranging from 0.94 to 1.00. No statistically significant differences were observed between LAMP and culture results (p > 0.05). LAMP may represent a valuable complementary method for routine environmental surveillance.
Keywords: loop-mediated isothermal amplification; healthcare-associated infections; nosocomial pathogens; environmental monitoring; hospital surfaces loop-mediated isothermal amplification; healthcare-associated infections; nosocomial pathogens; environmental monitoring; hospital surfaces

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MDPI and ACS Style

Marino, F.; Bonincontro, C.; Caligaris, L.; Derelitto, C.; Girolamini, L.; Cristino, S. Environmental Application of Newly Designed Loop-Mediated Isothermal Amplification (LAMP) Kits for Nosocomial Pathogen Detection in Hospital Settings. Life 2026, 16, 994. https://doi.org/10.3390/life16060994

AMA Style

Marino F, Bonincontro C, Caligaris L, Derelitto C, Girolamini L, Cristino S. Environmental Application of Newly Designed Loop-Mediated Isothermal Amplification (LAMP) Kits for Nosocomial Pathogen Detection in Hospital Settings. Life. 2026; 16(6):994. https://doi.org/10.3390/life16060994

Chicago/Turabian Style

Marino, Federica, Caterina Bonincontro, Laura Caligaris, Carlo Derelitto, Luna Girolamini, and Sandra Cristino. 2026. "Environmental Application of Newly Designed Loop-Mediated Isothermal Amplification (LAMP) Kits for Nosocomial Pathogen Detection in Hospital Settings" Life 16, no. 6: 994. https://doi.org/10.3390/life16060994

APA Style

Marino, F., Bonincontro, C., Caligaris, L., Derelitto, C., Girolamini, L., & Cristino, S. (2026). Environmental Application of Newly Designed Loop-Mediated Isothermal Amplification (LAMP) Kits for Nosocomial Pathogen Detection in Hospital Settings. Life, 16(6), 994. https://doi.org/10.3390/life16060994

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