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Article

Unveiling Gene Expression Dynamics during Early Embryogenesis in Cynoglossus semilaevis: A Transcriptomic Perspective

by
Xinyi Cheng
1,2,
Wei Jiang
3,
Qian Wang
4,5,
Kaiqiang Liu
4,5,
Wei Dai
2,
Yuyan Liu
4,5,
Changwei Shao
4,5,* and
Qiye Li
2,3,6,*
1
School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China
2
BGI Research, Wuhan 430074, China
3
BGI Research, Shenzhen 518083, China
4
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
5
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
6
College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
*
Authors to whom correspondence should be addressed.
Life 2024, 14(4), 505; https://doi.org/10.3390/life14040505
Submission received: 24 March 2024 / Revised: 10 April 2024 / Accepted: 11 April 2024 / Published: 15 April 2024
(This article belongs to the Section Genomics and Proteomics)
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Abstract

Commencing with sperm–egg fusion, the early stages of metazoan development include the cleavage and formation of blastula and gastrula. These early embryonic events play a crucial role in ontogeny and are accompanied by a dramatic remodeling of the gene network, particularly encompassing the maternal-to-zygotic transition. Nonetheless, the gene expression dynamics governing early embryogenesis remain unclear in most metazoan lineages. We conducted transcriptomic profiling on two types of gametes (oocytes and sperms) and early embryos (ranging from the four-cell to the gastrula stage) of an economically valuable flatfish–the Chinese tongue sole Cynoglossus semilaevis (Pleuronectiformes: Cynoglossidae). Comparative transcriptome analysis revealed that large-scale zygotic genome activation (ZGA) occurs in the blastula stage, aligning with previous findings in zebrafish. Through the comparison of the most abundant transcripts identified in each sample and the functional analysis of co-expression modules, we unveiled distinct functional enrichments across different gametes/developmental stages: actin- and immune-related functions in sperms; mitosis, transcription inhibition, and mitochondrial function in oocytes and in pre-ZGA embryos (four- to 1000-cell stage); and organ development in post-ZGA embryos (blastula and gastrula). These results provide insights into the intricate transcriptional regulation of early embryonic development in Cynoglossidae fish and expand our knowledge of developmental constraints in vertebrates.
Keywords: Cynoglossus semilaevis; Cynoglossidae; gametes; early embryonic development; transcriptomics Cynoglossus semilaevis; Cynoglossidae; gametes; early embryonic development; transcriptomics

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MDPI and ACS Style

Cheng, X.; Jiang, W.; Wang, Q.; Liu, K.; Dai, W.; Liu, Y.; Shao, C.; Li, Q. Unveiling Gene Expression Dynamics during Early Embryogenesis in Cynoglossus semilaevis: A Transcriptomic Perspective. Life 2024, 14, 505. https://doi.org/10.3390/life14040505

AMA Style

Cheng X, Jiang W, Wang Q, Liu K, Dai W, Liu Y, Shao C, Li Q. Unveiling Gene Expression Dynamics during Early Embryogenesis in Cynoglossus semilaevis: A Transcriptomic Perspective. Life. 2024; 14(4):505. https://doi.org/10.3390/life14040505

Chicago/Turabian Style

Cheng, Xinyi, Wei Jiang, Qian Wang, Kaiqiang Liu, Wei Dai, Yuyan Liu, Changwei Shao, and Qiye Li. 2024. "Unveiling Gene Expression Dynamics during Early Embryogenesis in Cynoglossus semilaevis: A Transcriptomic Perspective" Life 14, no. 4: 505. https://doi.org/10.3390/life14040505

APA Style

Cheng, X., Jiang, W., Wang, Q., Liu, K., Dai, W., Liu, Y., Shao, C., & Li, Q. (2024). Unveiling Gene Expression Dynamics during Early Embryogenesis in Cynoglossus semilaevis: A Transcriptomic Perspective. Life, 14(4), 505. https://doi.org/10.3390/life14040505

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