Eicosapentaenoic Acid Induces the Inhibition of Adipogenesis by Reducing the Effect of PPARγ Activator and Mediating PKA Activation and Increased COX-2 Expression in 3T3-L1 Cells at the Differentiation Stage
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Adipogenesis Induction by 3T3-L1 Cell Culture
2.3. Measurement of Intracellular Triacylglycerols and Proteins
2.4. Visualization of Intracellular Lipids by Oil Red O Staining
2.5. Measurement of Gene Expression Levels
2.6. Data Presentation and Statistical Analysis
3. Results
3.1. Addition of EPA during the Differentiation Stage Effectively Inhibited Adipogenesis Evoked by MDI
3.2. Adipocyte-Specific Marker Genes Expression during the Differentiation Stage When 3T3-L1 Cells Are Cultured with EPA or AA
3.3. Δ12-PGJ2, a PPARγ Activator, Partially Attenuates the Anti-Adipogenic Effect of EPA
3.4. The Impact of Protein Kinase A (PKA) Activation on the Effect of EPA Added Exclusively during the Differentiation Stage of 3T3-L1 Cells on Adipogenesis Evoked by the MDI Cocktail
3.5. EPA-Induced COX-2 Expression during the Differentiation Stage
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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GenBank Accession No. | Target Genes | Primers (5→3′) | Length (bp) | Tm (°C) | Product Length (bp) |
---|---|---|---|---|---|
NM_008493 | Leptin | F: TTTCACACACGCAGTCGGTA | 20 | 59.90 | 149 |
R: CACATTTTGGGAAGGCAGGC | 20 | 60.04 | |||
AB008453 | Glut4 | F: GGATTCCATCCCACAAGGCA | 20 | 60.03 | 158 |
R: CCAACACGGCCAAGACATTG | 20 | 60.04 | |||
NM_011198.5 | COX-2 | F: GTTTGTTGAGTCATTCACCAG | 21 | 55.36 | 224 |
R: TGTAGAGGGCTTTCAATTCTG | 21 | 55.59 | |||
NM_007393.5 | β-Actin | F: GCGGGCGACGATGCT | 15 | 59.84 | 197 |
R: TGCCAGATCTTCTCCATGTCG | 21 | 59.86 |
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Nartey, M.N.N.; Shimizu, H.; Sugiyama, H.; Higa, M.; Syeda, P.K.; Nishimura, K.; Jisaka, M.; Yokota, K. Eicosapentaenoic Acid Induces the Inhibition of Adipogenesis by Reducing the Effect of PPARγ Activator and Mediating PKA Activation and Increased COX-2 Expression in 3T3-L1 Cells at the Differentiation Stage. Life 2023, 13, 1704. https://doi.org/10.3390/life13081704
Nartey MNN, Shimizu H, Sugiyama H, Higa M, Syeda PK, Nishimura K, Jisaka M, Yokota K. Eicosapentaenoic Acid Induces the Inhibition of Adipogenesis by Reducing the Effect of PPARγ Activator and Mediating PKA Activation and Increased COX-2 Expression in 3T3-L1 Cells at the Differentiation Stage. Life. 2023; 13(8):1704. https://doi.org/10.3390/life13081704
Chicago/Turabian StyleNartey, Michael N. N., Hidehisa Shimizu, Hikaru Sugiyama, Manami Higa, Pinky Karim Syeda, Kohji Nishimura, Mitsuo Jisaka, and Kazushige Yokota. 2023. "Eicosapentaenoic Acid Induces the Inhibition of Adipogenesis by Reducing the Effect of PPARγ Activator and Mediating PKA Activation and Increased COX-2 Expression in 3T3-L1 Cells at the Differentiation Stage" Life 13, no. 8: 1704. https://doi.org/10.3390/life13081704