IGFBP-6 Alters Mesenchymal Stromal Cell Phenotype Driving Dasatinib Resistance in Chronic Myeloid Leukemia
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Lines
2.2. XTT Assay
2.3. Western Blot Analysis
2.4. Immunofluorescence
2.5. RT-PCR Analysis
2.6. Statistical Analysis
3. Results
3.1. Dasatinib Exposure Does Not Affect LAMA-84 Cell Viability in Coculture with Human Mesenchymal Stem Cells (HS-5)
3.2. Dasatinib Increases IGFBP-6 Levels in Coculture Conditions and Pre-Treatment with IGFBP-6 Leads to Increased Cell Viability in LAMA-84 Cells
3.3. Pre-Treatment with IGFBP-6 Increases SHH Expression Levels in Coculture Models and Activates TLR-4 Signalling
3.4. The IGFBP-6-Dasatinib Combination Treatment Increases HS-5 Inflammatory State
3.5. The IGFBP-6 + Dasatinib Combination Treatment Increases LAMA-84 Inflammatory State and Activates TLR-4 Signalling
3.6. IGFBP-6 Rescues LAMA-84 Cell Viability after Dasatinib Exposure
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene of Interest | Forward Primer (5′ → 3′) | Reverse Primer (5′ → 3′) |
---|---|---|
SIRT1 | AGGCCACGGATAGGTCCATA | GTGGAGGTATTGTTTCCGGC |
PGC1α | ATGAAGGGTACTTTTCTGCCCC | GGTCTTCACCAACCAGAGCA |
TGF-β | CCCAGCATCTGCAAAGCTC | GTCAATGTACAGCTGCCGCA |
IFNγ | TGAATGTCCAACGCAAAGCA | CGACCTCGAAACAGCATCTGA |
IGFBP-6 | CCTGCTGTTGCAGAGGAGAAT | CTCTGCGGTTCACATCCTGT |
SHH | GCGAGATGTCTGCTGCTAGT | TTACACCTCTGAGTCATCAGC |
TLR4 | AAGCCGAAAGGTGATTGTTG | CTGAGCAGGGTCTTCTCCAC |
βActin | CCTTTGCCGATCCGCCG | AACATGATCTGGGTCATCTTCTCGC |
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Cambria, D.; Longhitano, L.; La Spina, E.; Giallongo, S.; Orlando, L.; Giuffrida, R.; Tibullo, D.; Fontana, P.; Barbagallo, I.; Nicoletti, V.G.; et al. IGFBP-6 Alters Mesenchymal Stromal Cell Phenotype Driving Dasatinib Resistance in Chronic Myeloid Leukemia. Life 2023, 13, 259. https://doi.org/10.3390/life13020259
Cambria D, Longhitano L, La Spina E, Giallongo S, Orlando L, Giuffrida R, Tibullo D, Fontana P, Barbagallo I, Nicoletti VG, et al. IGFBP-6 Alters Mesenchymal Stromal Cell Phenotype Driving Dasatinib Resistance in Chronic Myeloid Leukemia. Life. 2023; 13(2):259. https://doi.org/10.3390/life13020259
Chicago/Turabian StyleCambria, Daniela, Lucia Longhitano, Enrico La Spina, Sebastiano Giallongo, Laura Orlando, Rosario Giuffrida, Daniele Tibullo, Paolo Fontana, Ignazio Barbagallo, Vincenzo Giuseppe Nicoletti, and et al. 2023. "IGFBP-6 Alters Mesenchymal Stromal Cell Phenotype Driving Dasatinib Resistance in Chronic Myeloid Leukemia" Life 13, no. 2: 259. https://doi.org/10.3390/life13020259