Circadian Rhythm Regulates Reactive Oxygen Species Production and Inhibits Al-Induced Programmed Cell Death in Peanut
Abstract
:1. Introduction
2. Results
2.1. Effects of Photoperiod and Al Stress on Root Elongation and Cell Death
2.2. Effects of Photoperiod and Al Stress on Al Accumulation in the Root Tips and CW
2.3. Effects of Photoperiod and Al Stress on MDA Content, PME Activity, and LOX Activity
2.4. Effects of Photoperiod and Al on ROS Production and Antioxidant Enzyme Activities
2.5. Effect of Photoperiod on AhLHY and AhSTS Gene Expression under Al Stress
3. Discussion
3.1. Effects of Photoperiods on Root Elongation and Cell Death under Al Stress
3.2. Effect of Photoperiod and Al Stress on MDA, PME, and LOX Activity
3.3. Circadian Rhythm Regulates Al-Induced PCD by Controlling ROS Production and Antioxidant Enzyme Activity
3.4. Effect of Photoperiod and Al Stress on AhLHY and AhSTS Gene Expression
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Relative Root Growth, Evan’s Blue Staining, and Cell Death Assay
4.3. Al Content in Root Tips and Cell Wall
4.4. Lipid Peroxidation and PME Activity Assay
4.5. ROS (H2O2 and O2.−) Content
4.6. Antioxidant Enzyme Activity
4.7. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) Analysis
Gene Name | Gene ID | Primer |
AhLHY-forward | AH02G04520.1 | 5′-ATTGACTCTAGTAATCGTCGTA-3′ |
AhLHY-reverse | 5′-CTTTGTGGCAACACCTCT-3′ | |
AhSTS-forward | AH04G08660.1 | 5′-CCCAAGCGTCAAGAGGTA-3′ |
AhSTS-reverse | 5′-TTGCCCACAAGACTATCCA-3′ | |
Actin-forward | AH03G02610.1 | 5′-ACCTTCTACAACGAGCTTCGTGTG-3′ |
Actin-reverse | 5′-GAAAGAACAGCCTGAATGGCAAC-3′ |
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Ntambiyukuri, A.; Li, X.; Xiao, D.; Wang, A.; Zhan, J.; He, L. Circadian Rhythm Regulates Reactive Oxygen Species Production and Inhibits Al-Induced Programmed Cell Death in Peanut. Life 2022, 12, 1271. https://doi.org/10.3390/life12081271
Ntambiyukuri A, Li X, Xiao D, Wang A, Zhan J, He L. Circadian Rhythm Regulates Reactive Oxygen Species Production and Inhibits Al-Induced Programmed Cell Death in Peanut. Life. 2022; 12(8):1271. https://doi.org/10.3390/life12081271
Chicago/Turabian StyleNtambiyukuri, Aaron, Xia Li, Dong Xiao, Aiqin Wang, Jie Zhan, and Longfei He. 2022. "Circadian Rhythm Regulates Reactive Oxygen Species Production and Inhibits Al-Induced Programmed Cell Death in Peanut" Life 12, no. 8: 1271. https://doi.org/10.3390/life12081271