Dl-3-n-Butylphthalide Reduced Neuroinflammation by Inhibiting Inflammasome in Microglia in Mice after Middle Cerebral Artery Occlusion
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Design
2.2. Establishment of the tMCAO Mouse Model
2.3. Neurobehavioral Tests Determination
2.4. Brain Infarct Volume Measurement
2.5. Immunohistochemical Staining
2.6. Western Blotting Analysis
2.7. Real-Time PCR
2.8. Cell Culture
2.9. Oxygen-Glucose Deprivation (OGD)
2.10. Cell Proliferation and Cytotoxicity Assay
2.11. Statistical Analysis
3. Results
3.1. NBP Reduced Infarct Volume and Neurological Behavior in tMCAO Mice
3.2. NBP Attenuated the Inflammatory Factor Expression after tMCAO
3.3. NBP Inhibited NLRP3 Inflammasome Expression in Microglia
3.4. NBP Treatment Reduced CASP1 Expression in Neurons
3.5. NBP Treatment Indirectly Reduced the Apoptosis of Neurons In Vitro
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer | Sequence (5′-3′) |
---|---|
TNF-α-F | TAGCCAGGAGGGAGAACAGA |
TNF-α-R | CCAGTGAGTGAAAGGGACAGA |
IL-1α-F | CGCTTGAGTCGGCAAAGAAAT |
IL-1α-R | CTTCCCGTTGCTTGACGTTG |
IL-1β-F | TACATCAGCACCTCACAAGC |
IL-1β-R | AGAAACAGTCCAGCCCATACT |
GAPDH-F | AAATGGTGAAGGTCGGTGTG |
GAPDH-R | AGGTCAATGAAGGGGTCGTT |
IL-6-F | ACCAAGACCATCCAATTCATC |
IL-6-R | CTGACCACAGTGAGGAATGTC |
IL-17-F | GTTCGTGCTATTGATTTTCAGC |
IL-17-R | GGACCCCTTTACACCTTCTTT |
IL-18-F | AGGACACTTTCTTGCTTGCCA |
IL-18-R | CACAAACCCTCCCCACCTAAC |
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Liu, M.; Zheng, H.; Liu, Z.; Guo, Y.; Wang, S.; Tang, Y.; Tian, H.; Zhang, Z.; Yang, G. Dl-3-n-Butylphthalide Reduced Neuroinflammation by Inhibiting Inflammasome in Microglia in Mice after Middle Cerebral Artery Occlusion. Life 2022, 12, 1244. https://doi.org/10.3390/life12081244
Liu M, Zheng H, Liu Z, Guo Y, Wang S, Tang Y, Tian H, Zhang Z, Yang G. Dl-3-n-Butylphthalide Reduced Neuroinflammation by Inhibiting Inflammasome in Microglia in Mice after Middle Cerebral Artery Occlusion. Life. 2022; 12(8):1244. https://doi.org/10.3390/life12081244
Chicago/Turabian StyleLiu, Mengdi, Haoran Zheng, Ze Liu, Yiyan Guo, Shuhong Wang, Yaohui Tang, Hengli Tian, Zhijun Zhang, and Guoyuan Yang. 2022. "Dl-3-n-Butylphthalide Reduced Neuroinflammation by Inhibiting Inflammasome in Microglia in Mice after Middle Cerebral Artery Occlusion" Life 12, no. 8: 1244. https://doi.org/10.3390/life12081244