Time-Course of Redox Status, Redox-Related, and Mitochondrial-Dynamics-Related Gene Expression after an Acute Bout of Different Physical Exercise Protocols
Abstract
:1. Introduction
2. Methods
2.1. Animals
2.2. Exercise Protocol
2.3. Determination of Total Peroxidase and Glutathione Peroxidase Activities in Muscle Samples
2.4. Determination of Citrate Synthase (CS) Activity
2.5. Quantitation of mRNA by RT-qPCR
2.6. Statistical Analysis
3. Results
3.1. Serum Lactate, TBARS, and Enzymatic Activity (Total Perosidase, GPx, and CS)
3.2. Mitochondrial Dynamics-Related Gene Expression in Muscle Tissue Samples
3.3. Redox-Related Gene Expression in Muscle Tissue Samples
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Identification | Reference Code | Protein |
---|---|---|---|
Gapdh | Mm99999915_g1 | 4453320 | GAPDH |
Ppargc1α | Mm01208835_m1 | 4453320 | PGC1-α |
Mfn2 | Mm00500120_m1 | 4453320 | MFN2 |
Dnm1L | Mm01342903_m1 | 4448892 | Drp1 |
Park2 | Mm01323528_m1 | 4448892 | Parkin |
Nfe2l2 | Mm00477784_m1 | 4453320 | NRF-2 |
Gpx1 | Mm00656767_g1 | 4453320 | GPx1 |
Nos2 | Mm00440502_m1 | 4453320 | NOS-2 |
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Pires, R.A.; Correia, T.M.L.; Almeida, A.A.; Coqueiro, R.d.S.; Machado, M.; Teles, M.F.; Peixoto, Á.S.; Queiroz, R.F.; Pereira, R. Time-Course of Redox Status, Redox-Related, and Mitochondrial-Dynamics-Related Gene Expression after an Acute Bout of Different Physical Exercise Protocols. Life 2022, 12, 2113. https://doi.org/10.3390/life12122113
Pires RA, Correia TML, Almeida AA, Coqueiro RdS, Machado M, Teles MF, Peixoto ÁS, Queiroz RF, Pereira R. Time-Course of Redox Status, Redox-Related, and Mitochondrial-Dynamics-Related Gene Expression after an Acute Bout of Different Physical Exercise Protocols. Life. 2022; 12(12):2113. https://doi.org/10.3390/life12122113
Chicago/Turabian StylePires, Ramon Alves, Thiago Macedo Lopes Correia, Amanda Alves Almeida, Raildo da Silva Coqueiro, Marco Machado, Mauro Fernandes Teles, Álbert Souza Peixoto, Raphael Ferreira Queiroz, and Rafael Pereira. 2022. "Time-Course of Redox Status, Redox-Related, and Mitochondrial-Dynamics-Related Gene Expression after an Acute Bout of Different Physical Exercise Protocols" Life 12, no. 12: 2113. https://doi.org/10.3390/life12122113