Cytogenetic Effects in Patients after Computed Tomography Examination
Abstract
:1. Introduction
2. Materials and Methods
2.1. Participants
2.2. CT Scans
2.3. Peripheral Blood Culture for Unstable Chromosome Aberration Analysis
2.4. Statistical Analysis
3. Results
3.1. Unstable Chromosome Analysis
3.2. Dicentric Distribution in Patients after CT Examination
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- United Nations Scientific Committee on the Effects of Atomic Radiation. Sources and Effects of Ionizing Radiation: UNSCEAR 2008, Report to the General Assembly, with Scientific Annexes; United Nations Scientific Committee on the Effects of Atomic Radiation: New York, NY, USA, 2010; p. 463. [Google Scholar]
- Barkovsky, A.N.; Bratilova, A.A.; Kormanovskaya, T.A.; Akhmatdinov, R.R.; Akhmatdinov, R.R. Trends in the doses of the population of the Russian Federation in 2003–2018. Radiatsionnaya Gygiena Radiat. Hyg. 2019, 12, 96–122. (In Russian) [Google Scholar] [CrossRef]
- National Council on Radiation Protection and Measurements. Medical Radiation Exposure of Patients in the United States; NCRP Report No. 184; National Council on Radiation Protection and Measurements: Bethesda, MD, USA, 2019. [Google Scholar]
- Frija, G.; Damilakis, J.; Paulo, G.; Loose, R.; Vano, E. European Society of Radiology (ESR). Cumulative effective dose from recurrent CT examinations in Europe: Proposal for clinical guidance based on an ESR EuroSafe Imaging survey. Eur. Radiol. 2021, 31, 5514–5523. [Google Scholar] [CrossRef] [PubMed]
- Lin, E.C. Radiation risk from medical imaging. Mayo Clin. Proc. 2010, 85, 1142–1146. [Google Scholar] [CrossRef] [Green Version]
- Brenner, D.; Elliston, C.; Hall, E.; Berdon, W. Estimated risks of radiation-induced fatal cancer from pediatric CT. AJR Am. J. Roentgenol. 2001, 176, 289–296. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Meulepas, J.M.; Ronckers, C.M.; Smets, A.M.J.B.; Nievelstein, R.A.J.; Gradowska, P.; Lee, C.; Jahnen, A.; van Straten, M.; de Wit, M.-S.Y.; Zonnenberg, B.; et al. Radiation Exposure From Pediatric CT Scans and Subsequent Cancer Risk in The Netherlands. J. Natl. Cancer Inst. 2019, 111, 256–263. [Google Scholar] [CrossRef]
- Nikkilä, A.; Raitanen, J.; Lohi, O.; Auvinen, A. Radiation exposure from computerized tomography and risk of childhood leukemia: Finnish register-based case-control study of childhood leukemia (FRECCLE). Haematologica 2018, 103, 1873–1880. [Google Scholar] [CrossRef]
- Zhang, Y.; Chen, Y.; Huang, H.; Sandler, J.; Dai, M.; Ma, S.; Udelsman, R. Diagnostic radiography exposure increases the risk for thyroid microcarcinoma: A population-based case-control study. Eur. J. Cancer Prev. 2015, 24, 439–446. [Google Scholar] [CrossRef]
- Shao, Y.H.; Tsai, K.; Kim, S.; Wu, Y.J.; Demissie, K. Exposure to Tomographic Scans and Cancer Risks. JNCI Cancer Spectr. 2019, 14, pkz072. [Google Scholar] [CrossRef]
- Lee, Y.K.; Lee, S.; Lee, E.K.; Kim, H.C.; Kong, S.Y.; Cha, H.S.; Hwangbo, Y. Can computed tomography scanning in adults lead to an increased risk of thyroid cancer? A nationwide nested case-control study. Eur. Radiol. 2022, 32, 415–423. [Google Scholar] [CrossRef]
- National Research Council of the National Academies. Health Risks from Exposure to Low Levels of Ionizing Radiation: BEIR VII Phase 2; The National Academies Press: Washington, DC, USA, 2006; p. 245. [Google Scholar]
- Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies; IAEA: Vienna, Austria, 2011; p. 247.
- Bonassi, S.; Norppa, H.; Ceppi, M.; Stromberg, U.; Vermeulen, R.; Znaor, A.; Cebulska-Wasilewska, A.; Fabianova, E.; Fucic, A.; Gundy, S.; et al. Chromosomal aberration frequency in lymphocytes predicts the risk of cancer: Results from a pooled cohort study of 22 358 subjects in 11 countries. Carcinogenesis 2008, 29, 1178–1183. [Google Scholar] [CrossRef]
- Methodical Guidance 2.6.1.2944 “Control of Patient Effective Doses in Medical X-ray Examinations”; Rospotrebnadzor: Moscow, Russia, 2011; p. 23. (In Russian)
- Balonov, M.; Golikov, V.; Zvonova, I.; Chipiga, L.; Kalnitsky, S.; Sarycheva, S.; Vodovatov, A. Patient doses from medical examinations in Russia: 2009–2015. J. Radiol. Prot. 2017, 38, 121–139. [Google Scholar] [CrossRef] [Green Version]
- Löbrich, M.; Rief, N.; Kühne, M.; Heckmann, M.; Fleckenstein, J.; Rübe, C.; Uder, M. In vivo formation and repair of DNA double-strand breaks after computed tomography examinations. Proc. Natl. Acad. Sci. USA 2005, 102, 8984–8989. [Google Scholar] [CrossRef] [Green Version]
- Deperas, J.; Szluinska, M.; Deperas-Kaminska, M.; Edwards, A.; Lloyd, D.; Lindholm, C.; Romm, H.; Roy, L.; Moss, R.; Morand, J.; et al. CABAS: A freely available PC program for fitting calibration curves in chromosome aberration dosimetry. Radiat. Prot. Dosim. 2007, 124, 115–123. [Google Scholar] [CrossRef] [PubMed]
- Bender, M.A.; Preston, R.J.; Leonard, R.C.; Pyatt, B.E.; Gooch, P.C.; Shelby, M. Chromosomal aberration and sister-chromatid exchange frequencies in peripheral blood lymphocytes of a large human population sample. Mutat. Res. Genet. 1988, 3, 421–433. [Google Scholar] [CrossRef]
- Golikov, V.Y. Evaluation of the radiation risk of medical examinations in the Russian Federation taking into account the age and sex distribution of the patients. Radiatsionnaya Gygiena = Radiat. Hyg. 2022, 15, 59–67. (In Russian) [Google Scholar]
- Unsertanties in the Estimation Risk and Probability of Desease Causation; NCRP Report No. 171; National Council of Radiation Protection and Measurements (NCRP): Bethesda, MD, USA, 2012; p. 418.
- Koppen, G.; Verheyen, G.; Maes, A.; Van Gorp, U.; Schoeters, G.; Hond, E.D.; Staessen, J.; Nawrot, T.; Roels, H.A.; Vlietinck, R.; et al. A battery of DNA effect biomarkers to evaluate environmental exposure of Flemish adolescents. J. Appl. Toxicol. 2007, 27, 238–246. [Google Scholar] [CrossRef] [PubMed]
- Abe, Y.; Miura, T.; Yoshida, M.; Ujiie, R.; Kurosu, Y.; Kato, N.; Katafuchi, A.; Tsuyama, N.; Ohba, T.; Inamasu, T.; et al. Increase in dicentric chromosome formation after a single CT scan in adults. Sci. Rep. 2015, 5, 13882. [Google Scholar] [CrossRef] [Green Version]
- Brambilla, M.; Cannillo, B.; D’Alessio, A.; Matheoud, R.; Agliata, M.F.; Carriero, A. Patients undergoing multiphase CT scans and receiving a cumulative effective dose of ≥ 100 mSv in a single episode of care. Eur. Radiol. 2021, 31, 4452–4458. [Google Scholar] [CrossRef] [PubMed]
- Rehani, M.M.; Yang, K.; Melick, E.R.; Heil, J.; Šalát, D.; Sensakovic, W.F.; Liu, B. Patients undergoing recurrent CT scans: Assessing the magnitude. Eur. Radiol. 2019, 30, 1828–1836. [Google Scholar] [CrossRef]
- Awa, A.A. Persistent chromosome aberrations in the somatic cells of A-bomb survivors, Hiroshima and Nagasaki. J. Radiat. Res. 1991, 32 (Suppl. S1), 265–274. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Ramalho, A.T.; Curado, M.P.; Natarajan, A.T. Results of a cytogenetic follow-up study 7,5 yr after 137Cs exposure at the Goiania (Brazil) radiological accident. Radiat. Prot. Dosm. 1996, 64, 319–321. [Google Scholar] [CrossRef]
- Neronova, E.; Slozina, N.; Nikiforov, A. Chromosome alterations in cleanup workers sampled years after the Chernobyl accident. Radiat. Res. 2003, 160, 46–51. [Google Scholar] [CrossRef] [PubMed]
- Tanaka, K.; Ohtaki, M.; Hoshi, M. Chromosome aberrations in Japanese fishermen exposed to fallout radiation 420-1200 km distant from the nuclear explosion test site at Bikini Atoll: Report 60 years after the incident. Radiat. Environ. Biophys. 2016, 55, 329–337. [Google Scholar] [CrossRef] [PubMed]
- Golfier, S.; Jost, G.; Pietsch, H.; Lengsfeld, P.; Eckardt-Schupp, F.; Schmid, E.; Voth, M. Dicentric chromosomes and gamma-H2AX foci formation in lymphocytes of human blood samples exposed to a CT scanner: A direct comparison of dose response relationships. Radiat. Prot. Dosim. 2009, 134, 55–61. [Google Scholar] [CrossRef]
- Kuefner, M.A.; Grudzenski, S.; Hamann, J.; Achenbach, S.; Lell, M.; Anders, K.; Schwab, S.A.; Häberle, L.; Löbrich, M.; Uder, M. Effect of CT scan protocols on x-ray-induced DNA double-strand breaks in blood lymphocytes of patients undergoing coronary CT angiography. Eur. Radiol. 2010, 12, 2917–2924. [Google Scholar] [CrossRef]
- Beels, L.; Bacher, K.; Smeets, P.; Verstraete, K.; Vral, A.; Thierens, H. Dose-length product of scanners correlates with DNA damage in patients undergoing contrast CT. Eur. J. Radiol. 2012, 81, 1495–1499. [Google Scholar] [CrossRef] [PubMed]
- Fukumoto, W.; Ishida, M.; Sakai, C.; Tashiro, S.; Ishida, T.; Nakano, Y.; Tatsugami, F.; Awai, K. DNA damage in lymphocytes induced by cardiac CT and comparison with physical exposure parameters. Eur. Radiol. 2017, 27, 1660–1666. [Google Scholar] [CrossRef] [Green Version]
- Yang, P.; Wang, S.; Liu, D.; Zhao, H.; Li, G. DNA double-strand breaks of human peripheral blood lymphocyte induced by CT examination of oral and maxillofacial region. Clin. Oral Investig. 2020, 24, 4617–4624. [Google Scholar] [CrossRef] [PubMed]
- Sakane, H.; Ishida, M.; Shi, L.; Fukumoto, W.; Sakai, C.; Miyata, Y.; Ishida, T.; Akita, T.; Okada, M.; Awai, K.; et al. Biological effects of low-dose chest CT on chromosomal DNA. Radiology 2020, 295, 439–445. [Google Scholar] [CrossRef]
- Abe, Y.; Noji, H.; Miura, T.; Sugai, M.; Kurosu, Y.; Ujiie, R.; Tsuyama, N.; Yanagi, A.; Yanai, Y.; Ohba, T.; et al. Investigation of the cumulative number of chromosome aberrations induced by three consecutive CT examinations in eight patients. J. Radiat. Res. 2019, 60, 729–739. [Google Scholar] [CrossRef] [Green Version]
- Kanagaraj, K.; Abdul Syed Basheerudeen, S.; Tamizh Selvan, G.; Jose, M.T.; Ozhimuthu, A.; Panneer Selvam, S.; Pattan, S.; Perumal, V. Assessment of dose and DNA damages in individuals exposed to low dose and low dose rate ionizing radiations during computed tomography imaging. Mutat. Res. Genet. Toxicol. Environ. Mutagen. 2015, 789–790, 1–6. [Google Scholar] [CrossRef] [PubMed]
- Shi, L.; Fujioka, K.; Sakurai-Ozato, N.; Fukumoto, W.; Satoh, K.; Sun, J.; Awazu, A.; Tanaka, K.; Ishida, M.; Ishida, T.; et al. Chromosomal Abnormalities in Human Lymphocytes after Computed Tomography Scan Procedure. Radiat. Res. 2018, 190, 424–432. [Google Scholar] [CrossRef] [PubMed]
N | Gender | Age | Part of Body Examined in CT | Effective Dose (mSv) | Scan with Contrast |
---|---|---|---|---|---|
1 | Female | 31 | Abdomen | 2.5 | No |
2 | Male | 32 | Abdomen | 3.1 | No |
3 | Female | 53 | Abdomen | 3.5 | No |
4 | Female | 33 | Abdomen | 3.7 | No |
5 | Male | 65 | Abdomen | 4.4 | Yes |
6 | Male | 45 | Abdomen | 4.7 | No |
7 | Male | 53 | Abdomen | 5.0 | No |
8 | Male | 49 | Abdomen | 8 | Yes |
9 | Male | 50 | Abdomen | 9.7 | Yes |
10 | Male | 44 | Abdomen | 9.9 | Yes |
11 | Male | 37 | Abdomen | 15 | Yes |
12 | Female | 42 | Chest | 1.5 | No |
13 | Female | 39 | Chest | 1.8 | No |
14 | Male | 71 | Chest | 2 | No |
15 | Male | 72 | Chest | 2 | No |
16 | Male | 70 | Chest | 2.2 | No |
17 | Male | 69 | Chest | 2.4 | No |
18 | Male | 54 | Chest | 2.6 | No |
19 | Male | 34 | Chest | 2.7 | No |
20 | Female | 51 | Chest | 2.8 | No |
21 | Female | 46 | Chest | 2.8 | No |
22 | Female | 72 | Chest | 2.8 | No |
23 | Female | 50 | Chest | 2.9 | No |
24 | Male | 54 | Chest | 3.0 | No |
25 | Male | 20 | Chest | 3.0 | No |
26 | Male | 40 | Chest | 3.0 | No |
27 | Male | 56 | Chest | 3.0 | No |
28 | Male | 45 | Chest | 4.0 | Yes |
29 | Male | 32 | Chest | 4.1 | Yes |
30 | Male | 48 | Chest | 4.1 | Yes |
31 | Male | 48 | Chest | 5.7 | Yes |
32 | Male | 42 | Chest | 5.8 | Yes |
33 | Male | 64 | Chest | 7.0 | Yes |
34 | Male | 64 | Chest | 7.0 | Yes |
35 | Female | 47 | Chest | 8.9 | Yes |
36 | Female | 74 | Chest | 9.2 | Yes |
37 | Female | 65 | Chest | 9.7 | Yes |
38 | Female | 55 | Chest | 9.8 | Yes |
39 | Male | 61 | Chest | 10.0 | Yes |
40 | Female | 52 | Brain | 2 | No |
41 | Female | 25 | Brain | 2.8 | No |
42 | Male | 46 | Brain | 3 | No |
Frequency | Exposed Group, N = 42, M ± SE, % | Control Group, N = 22, M ± SE, % |
---|---|---|
Chromatid-type aberrations | 0.59 ± 0.07 | 0.75 ± 0.20 |
Chromatid breaks | 0.54 ± 0.07 | 0.68 ± 0.20 |
Chromatid exchanges | 0.05 ± 0.01 | 0.07 ± 0.03 |
Chromosome-type aberrations | 0.96 ± 0.14 ** | 0.29 ± 0.08 |
Chromosome breaks | 0.38 ± 0.05 ** | 0.20 ± 0.08 |
Dicentrics + rings | 0.53 ± 0.12 ** | 0.11 ± 0.04 |
Total aberrations | 1.56 ± 0.14 * | 1.06 ± 0.21 |
N | Aberrations per Cell | Dispersion Index | U-Value | Poisson Distribution |
---|---|---|---|---|
1 | 0.002 | 2 | 31.83 | No |
2 | 0.003 | 3 | 57 | No |
3 | 0.004 | 2 | 22.89 | No |
4 | 0.005 | 2.2 | 29.9 | No |
5 | 0.006 | 2.2 | 28.3 | No |
6 | 0.006 | 4.3 | 83.1 | No |
7 | 0.006 | 4.3 | 83.1 | No |
8 | 0.008 | 2.6 | 32.1 | No |
9 | 0.007 | 1.32 | 7.62 | No |
10 | 0.01 | 4.26 | 76.9 | No |
11 | 0.01 | 3.76 | 67.4 | No |
12 | 0.034 | 4.22 | 72.8 | No |
13 | 0.016 | 1.19 | 3.45 | No |
14 | 0.017 | 5.16 | 82.15 | No |
15 | 0.044 | 6.58 | 62.44 | No |
16 | 0.006 | 3.33 | 57.94 | No |
17 | 0.0009 | 1 | 0 | Yes |
18 | 0.0009 | 1 | 0 | Yes |
19 | 0.0009 | 1 | 0 | Yes |
20 | 0.001 | 0.99 | −0.031 | Yes |
21 | 0.001 | 0.99 | −0.031 | Yes |
22 | 0.001 | 0.99 | −0.031 | Yes |
23 | 0.002 | 0.99 | −0.031 | Yes |
24 | 0.002 | 0.99 | −0.03 | Yes |
25 | 0.002 | 0.99 | −0.03 | Yes |
26 | 0.003 | 0.99 | −0.05 | Yes |
27 | 0.003 | 0.99 | −0.05 | Yes |
28 | 0.003 | 0.99 | −0.055 | Yes |
29 | 0.003 | 0.999 | −0.055 | Yes |
30 | 0.003 | 0.999 | −0.055 | Yes |
31 | 0.004 | 0.998 | −0.05 | Yes |
32 | 0.004 | 0.99 | −0.093 | Yes |
33 | 0.005 | 0.99 | −0.097 | Yes |
34 | 0.005 | 0.99 | −0.097 | Yes |
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Neronova, E.; Aleksanin, S. Cytogenetic Effects in Patients after Computed Tomography Examination. Life 2022, 12, 1983. https://doi.org/10.3390/life12121983
Neronova E, Aleksanin S. Cytogenetic Effects in Patients after Computed Tomography Examination. Life. 2022; 12(12):1983. https://doi.org/10.3390/life12121983
Chicago/Turabian StyleNeronova, Elizaveta, and Sergei Aleksanin. 2022. "Cytogenetic Effects in Patients after Computed Tomography Examination" Life 12, no. 12: 1983. https://doi.org/10.3390/life12121983