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Article

Establishment of an Intradermal Ear Injection Model of IL-17A and IL-36γ as a Tool to Investigate the Psoriatic Cytokine Network

1
Institute of Biochemistry and Molecular Biology, RWTH Aachen University, 52074 Aachen, Germany
2
Department of Dermatology and Allergology, Faculty of Medicine, RWTH Aachen University, 52074 Aachen, Germany
3
Interdisciplinary Center for Clinical Research (IZKF), Faculty of Medicine, RWTH Aachen University, 52074 Aachen, Germany
*
Author to whom correspondence should be addressed.
Equally contributing senior authors.
Academic Editors: Rolland Gyulai, José Manuel Carrascosa and Adam Reich
Life 2021, 11(8), 846; https://doi.org/10.3390/life11080846
Received: 31 May 2021 / Revised: 28 July 2021 / Accepted: 16 August 2021 / Published: 19 August 2021
Psoriasis is a chronic skin disease affecting 2–3% of the global population. The proinflammatory IL-17A is a key cytokine in psoriasis. Accumulating evidence has revealed that IL-36γ plays also a pathogenic role. To understand more precisely the role of the IL-17A–IL-36γ cytokine network in skin pathology, we used an ear injection model. We injected IL-17A or IL-36γ alone and in combination into the ear pinnae of mice. This resulted in a significant increase in ear thickness measured over time. Histological evaluation of IL-17A + IL-36γ-treated skin showed a strong acanthosis, hyperparakeratosis and infiltration of neutrophils. The same histological features were found in mice after injection of IL-36γ alone, but to a lesser extent. IL-17A alone was not able to induce psoriasis-like changes. Genes encoding proteins of the S100 family, antimicrobial peptides and chemo-attractants for neutrophils were upregulated in the IL-17A + IL-36γ group. A much weaker expression was seen after the injection of each cytokine alone. These results strengthen the hypothesis that IL-17A and IL-36γ drive psoriatic inflammation via a synergistic interaction. Our established intradermal ear injection model can be utilized in the future to monitor effects of various inhibitors of this cytokine network. View Full-Text
Keywords: psoriasis; mouse model; IL-17A; IL-36γ; ear injection psoriasis; mouse model; IL-17A; IL-36γ; ear injection
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MDPI and ACS Style

Kluwig, D.; Huth, S.; Abdallah, A.T.; Pfaff, C.M.; Fietkau, K.; Huth, L.; Marquardt, Y.; Baron, J.M.; Lüscher, B. Establishment of an Intradermal Ear Injection Model of IL-17A and IL-36γ as a Tool to Investigate the Psoriatic Cytokine Network. Life 2021, 11, 846. https://doi.org/10.3390/life11080846

AMA Style

Kluwig D, Huth S, Abdallah AT, Pfaff CM, Fietkau K, Huth L, Marquardt Y, Baron JM, Lüscher B. Establishment of an Intradermal Ear Injection Model of IL-17A and IL-36γ as a Tool to Investigate the Psoriatic Cytokine Network. Life. 2021; 11(8):846. https://doi.org/10.3390/life11080846

Chicago/Turabian Style

Kluwig, David, Sebastian Huth, Ali T. Abdallah, Carolina M. Pfaff, Katharina Fietkau, Laura Huth, Yvonne Marquardt, Jens M. Baron, and Bernhard Lüscher. 2021. "Establishment of an Intradermal Ear Injection Model of IL-17A and IL-36γ as a Tool to Investigate the Psoriatic Cytokine Network" Life 11, no. 8: 846. https://doi.org/10.3390/life11080846

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