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Article

Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-κB Signaling in CCD-986Sk Skin Fibroblasts

1
Department of Pathology, School of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Chungju 27478, Korea
2
Pharmacological Research Division, National Institute of Food and Drug Safety Evaluation, Osong Health Technology Administration Complex, Cheongju 28159, Korea
3
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon 34141, Korea
4
Department of Chemistry and Cosmetics, Jeju National University, Jeju 63243, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this paper.
Academic Editors: Magdolna Szanto and Attila Gabor Szollosi
Life 2021, 11(5), 427; https://doi.org/10.3390/life11050427
Received: 12 April 2021 / Revised: 4 May 2021 / Accepted: 6 May 2021 / Published: 10 May 2021
(This article belongs to the Special Issue Mechanisms Underlying Skin Pathologies)
Lipopolysaccharide (LPS)-induced inflammatory response leads to serious damage, up to and including tumorigenesis. Natural mineral sulfur, non-toxic sulfur (NTS), and methylsulfonylmethane (MSM) have anti-inflammatory activity that may inhibit LPS-induced inflammation. We hypothesized that sulfur compounds could inhibit LPS-induced inflammatory responses in CCD-986Sk skin fibroblasts. We used Western blotting and real-time PCR to analyze molecular signaling in treated and untreated cultures. We also used flow cytometry for cell surface receptor analysis, comet assays to evaluate DNA damage, and ELISA-based cytokine detection. LPS induced TLR4 activation and NF-κB signaling via canonical and protein kinase C (PKC)-dependent pathways, while NTS and MSM downregulated that response. NTS and MSM also inhibited LPS-induced nuclear accumulation and binding of NF-κB to proinflammatory cytokines COX-2, IL-1β, and IL-6. Finally, the sulfur compounds suppressed LPS-induced ROS accumulation and DNA damage in CCD-986Sk cells. These results suggest that natural sulfur compounds could be used to treat inflammation and may be useful in the development of cosmetics. View Full-Text
Keywords: CCD-986Sk; LPS; NTS; MSM; TLR4; NF-κB; ROS; anti-inflammatory effect CCD-986Sk; LPS; NTS; MSM; TLR4; NF-κB; ROS; anti-inflammatory effect
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MDPI and ACS Style

Sp, N.; Kang, D.Y.; Kim, H.D.; Rugamba, A.; Jo, E.S.; Park, J.-C.; Bae, S.W.; Lee, J.-M.; Jang, K.-J. Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-κB Signaling in CCD-986Sk Skin Fibroblasts. Life 2021, 11, 427. https://doi.org/10.3390/life11050427

AMA Style

Sp N, Kang DY, Kim HD, Rugamba A, Jo ES, Park J-C, Bae SW, Lee J-M, Jang K-J. Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-κB Signaling in CCD-986Sk Skin Fibroblasts. Life. 2021; 11(5):427. https://doi.org/10.3390/life11050427

Chicago/Turabian Style

Sp, Nipin, Dong Y. Kang, Hyoung D. Kim, Alexis Rugamba, Eun S. Jo, Jong-Chan Park, Se W. Bae, Jin-Moo Lee, and Kyoung-Jin Jang. 2021. "Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-κB Signaling in CCD-986Sk Skin Fibroblasts" Life 11, no. 5: 427. https://doi.org/10.3390/life11050427

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