Poria cocos Modulates Th1/Th2 Response and Attenuates Airway Inflammation in an Ovalbumin-Sensitized Mouse Allergic Asthma Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Poria cocos Extract (Lipucan®)
2.2. Animals
2.3. Establishment of the Experimental Animal Model of Asthma and Treatments
2.4. BronchoalveolarLavage Fluid (BALF) Analysis and Histological Analysis
2.5. Determination of Eotaxin, IL-5, LTC4, and PGE2 in BALF
2.6. Determination of Total IgE, IgA, IgM, IgG, and OVA-Specific IgE
2.7. Cytokine Levels in Splenocytesand BALF Cultured Supernatants
2.8. Evaluation of NK Cell Activity
2.9. Statistics
3. Results
3.1. P. cocos Reduced Inflammation in BALF and Lung Tissues in OVA-Sensitized Mice
3.2. P. cocos Suppressed the Release of Total IgE in Blood Serum
3.3. P. cocos Suppressed OVA-Induced Chemokine and Cytokine Secretion in BALF
3.4. P. cocos Effects on Cytokine Release in ConA-Stimulated Cultured Splenocytes Derived from OVA-Sensitized Mice
3.5. P. cocos Effects on Cytokine Release in OVA-Stimulated Cultured Splenocytes Derived from OVA-Sensitized Mice
3.6. P. cocos Effects on NK Cell Activity in OVA-Sensitized Mice
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chao, C.-L.; Wang, C.-J.; Huang, H.-W.; Kuo, H.-P.; Su, M.-H.; Lin, H.-C.; Teng, C.-W.; Sy, L.B.; Wu, W.-M. Poria cocos Modulates Th1/Th2 Response and Attenuates Airway Inflammation in an Ovalbumin-Sensitized Mouse Allergic Asthma Model. Life 2021, 11, 372. https://doi.org/10.3390/life11050372
Chao C-L, Wang C-J, Huang H-W, Kuo H-P, Su M-H, Lin H-C, Teng C-W, Sy LB, Wu W-M. Poria cocos Modulates Th1/Th2 Response and Attenuates Airway Inflammation in an Ovalbumin-Sensitized Mouse Allergic Asthma Model. Life. 2021; 11(5):372. https://doi.org/10.3390/life11050372
Chicago/Turabian StyleChao, Chien-Liang, Chao-Jih Wang, Hsin-Wen Huang, Han-Peng Kuo, Muh-Hwan Su, Hang-Ching Lin, Chia-Wen Teng, Leticia B. Sy, and Wen-Mein Wu. 2021. "Poria cocos Modulates Th1/Th2 Response and Attenuates Airway Inflammation in an Ovalbumin-Sensitized Mouse Allergic Asthma Model" Life 11, no. 5: 372. https://doi.org/10.3390/life11050372