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Communication

Lectin Staining of Microvascular Glycocalyx in Microfluidic Cancer Cell Extravasation Assays

1
Department of Biomedical Engineering, The Chinese University of Hong Kong, New Territories, Hong Kong, China
2
Institute for Tissue Engineering and Regenerative Medicine, The Chinese University of Hong Kong, New Territories, Hong Kong, China
3
Faculty of Medicine, School of Biomedical Sciences, The Chinese University of Hong Kong, New Territories, Hong Kong, China
4
Department of Orthopaedics and Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China
5
Departments of Biological Engineering and Mechanical Engineering, Massachusetts Institute of Technology, 500 Technology Square, MIT Building, Room NE47-321, Cambridge, MA 02139, USA
*
Authors to whom correspondence should be addressed.
Academic Editors: Friedrich Jung and Ruth Lyck
Life 2021, 11(3), 179; https://doi.org/10.3390/life11030179
Received: 5 January 2021 / Revised: 17 February 2021 / Accepted: 18 February 2021 / Published: 25 February 2021
(This article belongs to the Special Issue 2020: A 10 Years Journey—Advances in Life Sciences)
The endothelial glycocalyx forms the inner-most lining of human microvasculature. It ensures the physiological function of blood vessels and plays a crucial role in the occurrence and progression of microvascular diseases. The present communication aims to highlight the usefulness of high-resolution imaging of lectin (Bandeiraea Simplicifolia) stained endothelial glycocalyx in 3-dimensional microfluidic cell cultures. The microfluidic system allowed visualizing cancer cell extravasation, which is a key event in metastasis formation in cancer pathologies. In brief, microvascular networks were created through spontaneous vasculogenesis. This occurred from 3 dimensional (3D) suspensions of human umbilical vein endothelial cells (HUVECs) in hydrogels confined within microfluidic devices. Extravasation of MDA-MB-231 breast cancer cells from perfusable endothelial lumens was observed with confocal imaging of lectin-stained microvascular networks. The present work provides guidance towards optimizing the methodology used to elucidate the role of the endothelial glycocalyx during cancer cell extravasation. In particular, a high-resolution view of the endothelial glycocalyx at the site of extravasation is presented. The occurrence of glycocalyx defects is well aligned with the contemporary notion in the field that glycocalyx shedding precedes cancer cell extravasation. View Full-Text
Keywords: endothelial glycocalyx; cancer cell extravasation; lectin staining endothelial glycocalyx; cancer cell extravasation; lectin staining
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MDPI and ACS Style

Beyer, S.; Blocki, A.; Cheung, M.C.Y.; Wan, Z.H.Y.; Mehrjou, B.; Kamm, R.D. Lectin Staining of Microvascular Glycocalyx in Microfluidic Cancer Cell Extravasation Assays. Life 2021, 11, 179. https://doi.org/10.3390/life11030179

AMA Style

Beyer S, Blocki A, Cheung MCY, Wan ZHY, Mehrjou B, Kamm RD. Lectin Staining of Microvascular Glycocalyx in Microfluidic Cancer Cell Extravasation Assays. Life. 2021; 11(3):179. https://doi.org/10.3390/life11030179

Chicago/Turabian Style

Beyer, Sebastian, Anna Blocki, Matthew Chung Yin Cheung, Zoe Ho Ying Wan, Babak Mehrjou, and Roger Dale Kamm. 2021. "Lectin Staining of Microvascular Glycocalyx in Microfluidic Cancer Cell Extravasation Assays" Life 11, no. 3: 179. https://doi.org/10.3390/life11030179

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