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Article

Chronic Stress and Gonadectomy Affect the Expression of Cx37, Cx40 and Cx43 in the Spinal Cord

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Department of Anatomy, Histology and Embryology, University of Split School of Medicine, Šoltanska 2, 21000 Split, Croatia
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Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 10/E, 31000 Osijek, Croatia
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Faculty of Science, University of Split, Ruđera Boškovića 33, 21000 Split, Croatia
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Department of Health Studies, University of Zadar, Splitska 1, 23000 Zadar, Croatia
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Department of Pharmacology and Pharmacotherapy, Interdisciplinary Excellence Centre, University of Szeged, Dóm Tér. 12., H-6720 Szeged, Hungary
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International Research and Innovation in Medicine Program, Cedars–Sinai Medical Center, Los Angeles, CA 90048, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Peter Claus and Katalin Prokai-Tatrai
Life 2021, 11(12), 1330; https://doi.org/10.3390/life11121330
Received: 8 September 2021 / Revised: 22 November 2021 / Accepted: 29 November 2021 / Published: 1 December 2021
(This article belongs to the Section Medical Research)
The study aimed to determine whether the exposure to chronic stress and/or performance of gonadectomy might lead to disturbance in the expression of connexin (Cx) 37, 40 and 43 in the spinal cord (SC), as a potential explanation for sex differences in stress-related chronic pain conditions. After the rats were sham-operated or gonadectomized, three 10-day sessions of sham or chronic stress were applied. Immunohistochemistry and transmission electron microscopy (TEM) were used to examine Cx localization and expression in the SC. The gonadectomy resulted in an increase of Cx37 expression in the dorsal horn (DH) of the female rats, but chronic stress suppressed the effects of castration. In male rats, only the combined effects of castration and chronic stress increased Cx37 expression. The influence of chronic stress on the DH Cx40 expression was inversely evident after the castration: increased in the ovariectomized female rats, while decreased in the orchidectomized male rats. We did not find any effect of chronic stress and castration, alone or together, on Cx43 expression in the DH, but the percentage of Cx43 overlapping the astrocyte marker glial fibrillary acidic protein (gfap) increased in the male stressed group after the castration. In conclusion, the association of the chronic stress with sex hormone depletion results in disturbances of the SC Cx expression and might be a possible mechanism of disturbed pain perception after chronic stress exposure. View Full-Text
Keywords: spinal cord; chronic stress; ovariectomy; orchidectomy; connexin spinal cord; chronic stress; ovariectomy; orchidectomy; connexin
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MDPI and ACS Style

Jurić, M.; Balog, M.; Ivić, V.; Benzon, B.; Racetin, A.; Bočina, I.; Kević, N.; Konjevoda, S.; Szűcs, K.F.; Gáspár, R.; Heffer, M.; Vukojević, K.; Vari, S.G.; Filipović, N. Chronic Stress and Gonadectomy Affect the Expression of Cx37, Cx40 and Cx43 in the Spinal Cord. Life 2021, 11, 1330. https://doi.org/10.3390/life11121330

AMA Style

Jurić M, Balog M, Ivić V, Benzon B, Racetin A, Bočina I, Kević N, Konjevoda S, Szűcs KF, Gáspár R, Heffer M, Vukojević K, Vari SG, Filipović N. Chronic Stress and Gonadectomy Affect the Expression of Cx37, Cx40 and Cx43 in the Spinal Cord. Life. 2021; 11(12):1330. https://doi.org/10.3390/life11121330

Chicago/Turabian Style

Jurić, Marija, Marta Balog, Vedrana Ivić, Benjamin Benzon, Anita Racetin, Ivana Bočina, Nives Kević, Suzana Konjevoda, Kálmán F. Szűcs, Róbert Gáspár, Marija Heffer, Katarina Vukojević, Sandor G. Vari, and Natalija Filipović. 2021. "Chronic Stress and Gonadectomy Affect the Expression of Cx37, Cx40 and Cx43 in the Spinal Cord" Life 11, no. 12: 1330. https://doi.org/10.3390/life11121330

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