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Open AccessArticle

Almond Skin Extracts and Chlorogenic Acid Delay Chronological Aging and Enhanced Oxidative Stress Response in Yeast

1
Laboratoire de Biologie des Ligneux et des Grandes Cultures, INRAE USC1328, University of Orleans, 45067 Orléans CEDEX 2, France
2
Bioactifs et Cosmetiques, CNRS GDR 3711, 45067 Orléans CEDEX 2, France
3
Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand
4
Laboratoire de Biologie des plantes et des micro-organismes, Faculté des Sciences, Université Mohamed Ier, Oujda 60000, Morocco
*
Authors to whom correspondence should be addressed.
These authors have equal contribution of the first authors.
These authors have equal contribution of the senior authors.
Life 2020, 10(6), 80; https://doi.org/10.3390/life10060080
Received: 23 April 2020 / Revised: 20 May 2020 / Accepted: 27 May 2020 / Published: 28 May 2020
Almond (Prunus dulcis (Mill.) D.A.Webb) is one of the largest nut crops in the world. Recently, phenolic compounds, mostly stored in almond skin, have been associated with much of the health-promoting behavior associated with their intake. The almond skin enriched fraction obtained from cold-pressed oil residues of the endemic Moroccan Beldi ecotypes is particularly rich in chlorogenic acid. In this study, both almond skin extract (AE) and chlorogenic acid (CHL) supplements, similar to traditional positive control resveratrol, significantly increased the chronological life-span of yeast compared to the untreated group. Our results showed that AE and CHL significantly reduced the production of reactive oxygen and nitrogen species (ROS/RNS), most likely due to their ability to maintain mitochondrial function during aging, as indicated by the maintenance of normal mitochondrial membrane potential in treated groups. This may be associated with the observed activation of the anti-oxidative stress response in treated yeast, which results in activation at both gene expression and enzymatic activity levels for SOD2 and SIR2, the latter being an upstream inducer of SOD2 expression. Interestingly, the differential gene expression induction of mitochondrial SOD2 gene at the expense of the cytosolic SOD1 gene confirms the key role of mitochondrial function in this regulation. Furthermore, AE and CHL have contributed to the survival of yeast under UV-C-induced oxidative stress, by reducing the development of ROS/RNS, resulting in a significant reduction in cellular oxidative damage, as evidenced by decreased membrane lipid peroxidation, protein carbonyl content and 8-oxo-guanine formation in DNA. Together, these results demonstrate the interest of AE and CHL as new regulators in the chronological life-span and control of the oxidative stress response of yeast. View Full-Text
Keywords: aging; almond; chlorogenic acid; lipid peroxidation; mitochondria; 8-Oxo-guanine; oxidative stress; protein carbonylation; sirtuin; superoxide dismutase; yeast aging; almond; chlorogenic acid; lipid peroxidation; mitochondria; 8-Oxo-guanine; oxidative stress; protein carbonylation; sirtuin; superoxide dismutase; yeast
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MDPI and ACS Style

Tungmunnithum, D.; Abid, M.; Elamrani, A.; Drouet, S.; Addi, M.; Hano, C. Almond Skin Extracts and Chlorogenic Acid Delay Chronological Aging and Enhanced Oxidative Stress Response in Yeast. Life 2020, 10, 80.

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