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Article

Repair Kinetics of DNA Double Strand Breaks Induced by Simulated Space Radiation

1
Department of Biological Sciences, College of Science, Ibaraki University, Mito, Ibaraki 310-8512, Japan
2
Department of Radiation-Applied Biology Research, Takasaki Advanced Radiation Research Institute, National Institutes for Quantum and Radiological Science and Technology (QST), Takasaki, Gunma 370-1292, Japan
*
Author to whom correspondence should be addressed.
Life 2020, 10(12), 341; https://doi.org/10.3390/life10120341
Received: 26 October 2020 / Revised: 4 December 2020 / Accepted: 9 December 2020 / Published: 10 December 2020
(This article belongs to the Special Issue Radiobiology in Space)
Radiation is unavoidable in space. Energetic particles in space radiation are reported to induce cluster DNA damage that is difficult to repair. In this study, normal human fibroblasts were irradiated with components of space radiation such as proton, helium, or carbon ion beams. Immunostaining for γ-H2AX and 53BP1 was performed over time to evaluate the kinetics of DNA damage repair. Our data clearly show that the repair kinetics of DNA double strand breaks (DSBs) induced by carbon ion irradiation, which has a high linear energy transfer (LET), are significantly slower than those of proton and helium ion irradiation. Mixed irradiation with carbon ions, followed by helium ions, did not have an additive effect on the DSB repair kinetics. Interestingly, the mean γ-H2AX focus size was shown to increase with LET, suggesting that the delay in repair kinetics was due to damage that is more complex. Further, the 53BP1 focus size also increased in an LET-dependent manner. Repair of DSBs, characterized by large 53BP1 foci, was a slow process within the biphasic kinetics of DSB repair, suggesting non-homologous end joining with error-prone end resection. Our data suggest that the biological effects of space radiation may be significantly influenced by the dose as well as the type of radiation exposure. View Full-Text
Keywords: space radiation; DNA double strand break; DNA repair; heavy ion; linear energy transfer space radiation; DNA double strand break; DNA repair; heavy ion; linear energy transfer
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MDPI and ACS Style

Oizumi, T.; Ohno, R.; Yamabe, S.; Funayama, T.; Nakamura, A.J. Repair Kinetics of DNA Double Strand Breaks Induced by Simulated Space Radiation. Life 2020, 10, 341. https://doi.org/10.3390/life10120341

AMA Style

Oizumi T, Ohno R, Yamabe S, Funayama T, Nakamura AJ. Repair Kinetics of DNA Double Strand Breaks Induced by Simulated Space Radiation. Life. 2020; 10(12):341. https://doi.org/10.3390/life10120341

Chicago/Turabian Style

Oizumi, Takashi, Rieko Ohno, Souichiro Yamabe, Tomoo Funayama, and Asako J. Nakamura 2020. "Repair Kinetics of DNA Double Strand Breaks Induced by Simulated Space Radiation" Life 10, no. 12: 341. https://doi.org/10.3390/life10120341

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