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Article

Expression Kinetics of Regulatory Genes Involved in the Vesicle Trafficking Processes Operating in Tomato Flower Abscission Zone Cells during Pedicel Abscission

1
Department of Postharvest Science, Agricultural Research Organization (ARO), The Volcani Center, Rishon LeZion 7528809, Israel
2
The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, Israel
*
Author to whom correspondence should be addressed.
Current Address: Department of Horticulture, Neelakudi Campus, School of Life Sciences, Central University of Tamil Nadu (CUTN), Thiruvarur-610 005, India.
Life 2020, 10(11), 273; https://doi.org/10.3390/life10110273
Received: 15 October 2020 / Revised: 31 October 2020 / Accepted: 3 November 2020 / Published: 6 November 2020
(This article belongs to the Section Plant Science)
The abscission process occurs in a specific abscission zone (AZ) as a consequence of the middle lamella dissolution, cell wall degradation, and formation of a defense layer. The proteins and metabolites related to these processes are secreted by vesicle trafficking through the plasma membrane to the cell wall and middle lamella of the separating cells in the AZ. We investigated this process, since the regulation of vesicle trafficking in abscission systems is poorly understood. The data obtained describe, for the first time, the kinetics of the upregulated expression of genes encoding the components involved in vesicle trafficking, occurring specifically in the tomato (Solanum lycopersicum) flower AZ (FAZ) during pedicel abscission induced by flower removal. The genes encoding vesicle trafficking components included soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), SNARE regulators, and small GTPases. Our results clearly show how the processes of protein secretion by vesicle trafficking are regulated, programmed, and orchestrated at the level of gene expression in the FAZ. The data provide evidence for target proteins, which can be further used for affinity purification of plant vesicles in their natural state. Such analyses and dissection of the complex vesicle trafficking networks are essential for further elucidating the mechanism of organ abscission. View Full-Text
Keywords: abscission; flower abscission zone; GTPases; SNAREs; tomato; transcriptome; vesicle trafficking abscission; flower abscission zone; GTPases; SNAREs; tomato; transcriptome; vesicle trafficking
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MDPI and ACS Style

Sundaresan, S.; Philosoph-Hadas, S.; Riov, J.; Salim, S.; Meir, S. Expression Kinetics of Regulatory Genes Involved in the Vesicle Trafficking Processes Operating in Tomato Flower Abscission Zone Cells during Pedicel Abscission. Life 2020, 10, 273. https://doi.org/10.3390/life10110273

AMA Style

Sundaresan S, Philosoph-Hadas S, Riov J, Salim S, Meir S. Expression Kinetics of Regulatory Genes Involved in the Vesicle Trafficking Processes Operating in Tomato Flower Abscission Zone Cells during Pedicel Abscission. Life. 2020; 10(11):273. https://doi.org/10.3390/life10110273

Chicago/Turabian Style

Sundaresan, Srivignesh, Sonia Philosoph-Hadas, Joseph Riov, Shoshana Salim, and Shimon Meir. 2020. "Expression Kinetics of Regulatory Genes Involved in the Vesicle Trafficking Processes Operating in Tomato Flower Abscission Zone Cells during Pedicel Abscission" Life 10, no. 11: 273. https://doi.org/10.3390/life10110273

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