Next Article in Journal / Special Issue
Efficient Expression of Antibody Fragments with the Brevibacillus Expression System
Previous Article in Journal
Kinetic Characterization of a Panel of High-Affinity Monoclonal Antibodies Targeting Ricin and Recombinant Re-Formatting for Biosensor Applications
Previous Article in Special Issue
The Biotechnological Applications of Recombinant Single-Domain Antibodies are Optimized by the C-Terminal Fusion to the EPEA Sequence (C Tag)

Refolding Technologies for Antibody Fragments

Alliance Protein Laboratories, 6042 Cornerstone Court West, San Diego, CA 92121, USA
Institute for Innovation, Ajinomoto Co. Inc., Kanagawa 210-8681, Japan
Author to whom correspondence should be addressed.
Antibodies 2014, 3(2), 232-241;
Received: 20 January 2014 / Revised: 26 April 2014 / Accepted: 13 May 2014 / Published: 23 May 2014
(This article belongs to the Special Issue Antibody Constructs)
Refolding is one of the production technologies for pharmaceutical grade antibody fragments. Detergents and denaturants are primarily used to solubilize the insoluble proteins. The solubilized and denatured proteins are refolded by reducing the concentration of the denaturants or detergents. Several refolding technologies have been used for antibody fragments, comprising dilution, dialysis, solid phase solvent exchange and size exclusion chromatography, as reviewed here. Aggregation suppressor or folding-assisting agents, including arginine hydrochloride, ionic liquids and detergents or denaturants at low concentrations, are included in the refolding solvent to enhance refolding yield. View Full-Text
Keywords: refolding; dialysis; dilution; C12-L-Glu; antibody; fragments refolding; dialysis; dilution; C12-L-Glu; antibody; fragments
MDPI and ACS Style

Arakawa, T.; Ejima, D. Refolding Technologies for Antibody Fragments. Antibodies 2014, 3, 232-241.

AMA Style

Arakawa T, Ejima D. Refolding Technologies for Antibody Fragments. Antibodies. 2014; 3(2):232-241.

Chicago/Turabian Style

Arakawa, Tsutomu, and Daisuke Ejima. 2014. "Refolding Technologies for Antibody Fragments" Antibodies 3, no. 2: 232-241.

Find Other Styles

Article Access Map by Country/Region

Only visits after 24 November 2015 are recorded.
Back to TopTop