Next Article in Journal
Reduced Culture Temperature Differentially Affects Expression and Biophysical Properties of Monoclonal Antibody Variants
Next Article in Special Issue
Antibody Fragments Defining Biologically Relevant Conformations of Target Proteins
Previous Article in Journal / Special Issue
Refolding Technologies for Antibody Fragments
 
 
Review

Efficient Expression of Antibody Fragments with the Brevibacillus Expression System

R&D Department, Higeta Shoyu Co. Ltd., 2-8 Chuo-cho, Choshi, Chiba 288-8680, Japan
*
Author to whom correspondence should be addressed.
Antibodies 2014, 3(2), 242-252; https://doi.org/10.3390/antib3020242
Received: 13 March 2014 / Revised: 2 May 2014 / Accepted: 12 May 2014 / Published: 26 May 2014
(This article belongs to the Special Issue Antibody Constructs)
Antibodies, owing to their capability to bind specifically to a target molecule, have been and will continue to be applied in various areas, including research, diagnosis and therapy. In particular, antibody fragments, which are size-reduced antibodies comprising functional variable domains, are suited for production in bacteria. They also are useful in applications requiring intracellular delivery and for further engineering toward molecules possessing multiple custom functions. An expression system based on Brevibacillus is characterized by high efficiency and simple genetic recombination for secretory production. The Brevibacillus expression system has been successfully utilized for the efficient production of antibody fragments, e.g., scFvs (single-chain antibody fragments) comprising heavy-chain and light-chain variable domains, linked by a spacer sequence. Expression in fusion with a Halobacterium-derived secretory protein was shown to confer enhanced productivity. In the case of Fabs, productivity as high as 100 mg/L was accomplished in a simple system, i.e., shake flask cultures. The Brevibacillus expression system offers several advantages, shared by other bacterial systems, such as E. coli, in particular, for the ease in genetic engineering and culture production. View Full-Text
Keywords: Brevibacillus expression system; antibody fragment; scFv; Fab Brevibacillus expression system; antibody fragment; scFv; Fab
Show Figures

Figure 1

MDPI and ACS Style

Hanagata, H.; Mizukami, M.; Miyauchi, A. Efficient Expression of Antibody Fragments with the Brevibacillus Expression System. Antibodies 2014, 3, 242-252. https://doi.org/10.3390/antib3020242

AMA Style

Hanagata H, Mizukami M, Miyauchi A. Efficient Expression of Antibody Fragments with the Brevibacillus Expression System. Antibodies. 2014; 3(2):242-252. https://doi.org/10.3390/antib3020242

Chicago/Turabian Style

Hanagata, Hiroshi, Makoto Mizukami, and Akira Miyauchi. 2014. "Efficient Expression of Antibody Fragments with the Brevibacillus Expression System" Antibodies 3, no. 2: 242-252. https://doi.org/10.3390/antib3020242

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop