1. Introduction
River-lake ecosystems are core carriers sustaining global hydrological cycles and ecological balance, playing irreplaceable strategic roles in regulating regional climate, ensuring water security, preserving biodiversity, and supporting socio-economic sustainable development [
1,
2,
3]. As a core technical support for ecological governance, river-lake health assessment systematically characterizes the structural integrity and functional stability of ecosystems through scientific indicator systems and evaluation methods [
4]. It provides targeted technical basis for accurately identifying ecological risks and formulating governance policies, with the reliability of its results directly influencing the rational allocation of ecological protection resources and the effectiveness of management measures [
5]. However, in practical assessment processes, affected by data availability and methodological limitations, most existing studies adopt multi-year averages or single-period measured values for indicator prediction, which tends to generate optimistic predicted values; meanwhile, weighting methods often prioritize indicators that directly reflect health status, leading to weight allocation that may amplify the optimistic tendency [
6,
7,
8]. Under this common tendency, the coupling of indicator prediction deviation and weight dispute will highly likely cause overestimation risk, which has become a common issue in current river-lake health assessment. This may lead to overestimation of the actual health level of ecosystems, further resulting in inadequate targeting of governance measures and failure of ecological risk prevention, threatening the long-term sustainable operation of river-lake systems. Therefore, constructing a risk analysis framework for river-lake health assessment that considers multiple uncertainties, and quantifying the probability and deviation degree of overestimation risk, holds significant theoretical value and practical significance for improving the scientificity of assessment results and enhancing the effectiveness of ecological governance decisions.
The global academic community has conducted extensive systematic research on river-lake health assessment, promoting the field’s continuous evolution from single-dimensional description to multi-dimensional, refined evaluation [
9]. In terms of indicator system construction, research has evolved from early single-dimensional evaluation focusing on water quality physicochemical indicators (e.g., dissolved oxygen, chemical oxygen demand) to comprehensive frameworks integrating natural and social dimensions [
10,
11,
12]. Representative systems such as the River and Lake Health Assessment Guidelines (EPA) and the “River Health Index” (Australia) have been developed globally, while researchers have also constructed evaluation systems tailored to the ecological characteristics of river-lakes in different regions by integrating multiple indicators including hydrology, water quality, biology, landscape, and social services [
13,
14,
15]. The core idea is to improve the comprehensiveness and representativeness of evaluation by fully covering key ecosystem processes. In terms of indicator weighting methods, three mainstream approaches have been formed: subjective weighting, objective weighting, and integrated weighting. Subjective methods, represented by the Analytic Hierarchy Process (AHP) and Delphi method, fully integrate expert knowledge of watershed ecological characteristics and management priorities. Objective methods, centered on the Entropy Weight Method (EWM) and coefficient of variation method, determine indicator importance based on statistical characteristics of monitoring data [
16,
17,
18,
19]. Some studies have attempted to balance the advantages of subjective experience and objective data through combined weighting methods [
20]. In terms of evaluation models, traditional methods such as fuzzy comprehensive evaluation and gray relational analysis have gradually been supplemented by artificial intelligence technologies including machine learning and neural networks, enabling dynamic simulation and prediction of river-lake health status [
21,
22,
23]. Meanwhile, methods such as Monte Carlo (MC) simulation and risk matrices have been applied in uncertainty analysis, advancing evaluation from “deterministic conclusions” to “probabilistic descriptions” [
24]. These studies have provided important technical support for global river-lake ecological protection and governance, effectively promoting the improvement and sustainable utilization of ecosystem service functions.
In the field of river-lake health assessment, overestimation risk specifically refers to the systematic deviation that the evaluated health level is significantly higher than the real ecological status of river-lake ecosystems. This risk is caused by the coupling of predictive uncertainty of indicator values and methodological uncertainty of weights, which will directly mislead ecological governance decisions, reduce the effectiveness of protection measures, cause the misallocation of ecological protection resources, and ultimately threaten the long-term sustainable operation of river-lake systems. At present, the overestimation risk has not been paid enough attention in existing studies. The lack of quantitative risk analysis will lead to unreliable assessment results and invalid governance decisions. Therefore, it is urgent to carry out targeted research on overestimation risk, which is of great theoretical value and practical significance to improve the scientificity of river-lake health assessment and the effectiveness of ecological governance.
There remain critical research gaps in the coupled analysis of multiple uncertainties and the quantification of overestimation risk in assessment processes, which are core bottlenecks in current research: (1) Insufficient quantification of indicator value uncertainty. River-lake ecological indicators are affected by multiple factors such as seasonal hydrological fluctuations, extreme weather events, and human activity disturbances, showing significant spatiotemporal variability. However, most existing studies adopt deterministic data such as multi-year averages or single-period measured values for evaluation, lacking systematic characterization of the predictive uncertainty of indicator values. Even when some studies use models to predict indicator values, they fail to fully consider the deviation distribution characteristics between historical predictions and actual values, resulting in evaluation results that cannot reflect the dynamic fluctuation laws of ecosystems. (2) Incomplete consideration of weight uncertainty. Different weighting methods have inherent differences in theoretical foundations and data dependence—subjective methods focus on expert experience judgments, while objective methods rely on statistical characteristics of data—often leading to conflicting weight results. Although existing studies mostly obtain a single coordinated weight through combined weighting, they lack quantitative description of the uncertainty of weights themselves (e.g., weight fluctuations caused by expert judgment deviations and data variability), ignoring the potential impact of weight uncertainty on evaluation results. (3) Unclear coupled mechanism between dual uncertainties and overestimation risk. Most existing studies analyze the uncertainty of indicator values or weights in isolation, lacking effective methods to couple the two and quantify their impact on the overestimation risk of evaluation results. Even though some studies recognize the existence of uncertainty, they have not established a quantitative relationship model between uncertainty and overestimation risk, making it impossible to accurately identify high-risk indicators and comprehensive risk levels. These gaps result in existing evaluation methods being unable to avoid decision-making risks caused by overestimation risk, restricting the practical application effectiveness of river-lake health assessment in complex ecological governance scenarios.
Purely measured statistical data can only reflect the ecological status of monitored years, while long-term river-lake health assessment is often hindered by discontinuous monitoring, missing historical sampling records and inconsistent monitoring frequencies. The predicted values of historical indicators solve the above problems from three aspects. Firstly, they fill the data gaps of years without field monitoring, and form an integrated continuous time series for multi-year comparative evaluation. Secondly, combined predicted and observed data can separate regular natural periodic fluctuations from abnormal degradation caused by human activities, avoiding misjudgment of the main factors affecting river-lake health. Thirdly, the complete historical series supported by prediction values can support trend extrapolation, which helps to discover latent ecological deterioration risks in advance and enhance the practical guiding significance of assessment results for watershed governance.
To address the aforementioned research gaps, this paper develops a general framework combining dual uncertainty quantification and overestimation risk coupling analysis with three key innovations. First, we build a standardized dual uncertainty quantification system for indicators and weights: PINN models reconstruct historical indicators and quantify prediction uncertainty, while Nash equilibrium game theory unifies multi-source weights, with weight uncertainty quantified via normal distribution assumptions. Second, a dual uncertainty–overestimation risk coupled model is established using the efficient FOSM method. Derived risk formulas quantify single and overall evaluation overestimation risks and characterize the overestimation risk induced by overlapping dual uncertainties. Third, the framework is validated via a Poyang Lake case study, which identifies high-risk indicators (TP, TN, FBLI) and generates risk zoning maps to support watershed management. This research remedies the deficiency of dual uncertainty–risk coupling research and forms an integrated technical pipeline from uncertainty measurement to decision assistance, providing a new research paradigm for scientific river-lake health evaluation.
3. Study Area
3.1. Study Area Overview
Poyang Lake, the largest freshwater lake in China, is geographically situated between 28°22′–29°45′ N latitude and 115°47′–116°45′ E longitude in northern Jiangxi Province (
Figure 5). Its catchment spans 162,200 km
2, covering most of Jiangxi Province and parts of Hubei, Anhui, Fujian, Zhejiang, and Guangdong provinces, with the lake’s northern outlet directly connecting to the Yangtze River mainstem. As a typical subtropical seasonal lake, it exhibits pronounced hydrological dynamism: the water surface area varies from 4741 km
2 at a water level of 21 m (flood season) to 225 km
2 at 12 m (dry season), representing a 20-fold fluctuation driven by monsoonal precipitation and Yangtze River backwater effects. This hydrological complexity forms a hierarchical river-lake system fed by five major tributaries (Ganjiang, Fuhe, Xinjiang, Raohe, and Xiuhe), underscoring its role as a core component of the Yangtze River Basin’s ecological security barrier.
Ecologically, Poyang Lake provides critical ecosystem services, including flood regulation (retaining an average of 30 billion m3 of floodwater annually), water supply for 10 million residents and 2 million mu of irrigated farmland, biodiversity conservation (supporting over 130 fish species and serving as a key wintering ground for >500,000 migratory birds, including the critically endangered Siberian crane), and regional climate modulation through latent heat exchange. However, in recent decades, anthropogenic pressures (e.g., agricultural non-point source pollution, sand mining, and water resource development) and climate change have induced significant ecological degradation. Monitoring data from 2018 to 2020 indicate persistent eutrophication (total phosphorus [TP] concentrations averaging 0.08 mg/L, total nitrogen [TN] 1.2 mg/L, and chlorophyll-a [Chl-a] 7.6 μg/L), coupled with reduced wetland area, degraded aquatic vegetation coverage, and declining biological integrity. These challenges highlight the necessity of robust health assessment frameworks to guide evidence-based ecological governance. Given its ecological significance, well-documented environmental challenges, and the need to address assessment uncertainties, Poyang Lake was selected as the case study area.
3.2. Multi-Dimensional Assessment Framework
A core prerequisite for river-lake health assessment lies in establishing an assessment indicator system that accurately reflects system complexity—a consensus widely recognized in the field of aquatic ecological governance. This consensus emphasizes three fundamental requirements for assessment indicator systems: dimension comprehensiveness (covering natural–social interaction processes), selection objectivity (free from reliance on subjective experience), and application adaptability (aligning with basin management practices). However, significant practical flaws persist in current assessment indicator system construction: most studies rely on expert subjective weighting or direct adoption of indicator combinations from single cases, lacking a quantitative screening process based on large-sample data. This leads to an experience-driven characteristic in indicator design—either excessive weighting of certain environmental factors due to high information redundancy (e.g., using a single organic pollution indicator to characterize both oxygen-consuming organic load and eutrophication potential) or one-sided assessment resulting from the omission of key dimensions such as satisfaction status of the lake’s minimum ecological water level (LMEL-S) and water resource development and utilization rate (WRDUR). A more prominent issue is the frequent separation between evaluation modules of natural systems (hydrology, water quality, aquatic biology) and social service functions, which fails to capture the evolutionary laws of river-lake health under the interaction of these two systems (e.g., the crowding-out effect of water resource development intensity on ecological baseflow satisfaction is not integrated into the assessment framework).
To address these practical challenges, this study adopted a Meta-analysis approach to systematically integrate 160 river-lake health assessment-related studies published in the Web of Science database from 1970 to 2024, constructing a standardized assessment indicator pool. The assessment indicator system was optimized through a three-step quantitative screening process: ① Frequency statistics to identify 45 core assessment indicators with relatively high occurrence frequencies (including total phosphorus (TP), total organic carbon (TOC), Fish Biological Loss Index (FBLI), water function zone compliance index (WFZCI), etc.); ② Pearson correlation analysis to eliminate redundant indicators with high information redundancy (e.g., removing indicators highly correlated with total nitrogen (TN) to avoid nutrient information overlap); ③ Correlation verification to select indicators strongly associated with river-lake health status. Ultimately, a comprehensive framework covering four criterion layers was established, and 12 key assessment indicators were determined after redundancy reduction and optimization (structure shown in
Figure 6): Hydrology and Water Resources (LMEL-S, satisfaction status of the ecological baseflow of major inflowing rivers (EBI-S)); Water Quality (oxygen-consuming organic pollution—permanganate index (CODMn)/ammonia nitrogen (NH3-N); water eutrophication status—TP/TN/chlorophyll a (Chl-a)/transparency (Trans)/TOC); Aquatic Biology (FBLI); Social Service Functions (WRDUR, WFZCI).
The innovative value of this assessment indicator system is reflected through specific technical pathways and practical effects: ① Based on the systematic integration of 160 studies and multi-round quantitative screening, the system effectively reduces indicator information redundancy while achieving full coverage of four core modules (hydrology, water quality, aquatic biology, and social service functions). By replacing redundant organic pollution indicators with eutrophication-specific TOC, it resolves the structural contradiction of “either redundancy or missing dimensions” in traditional indicator systems, forming a logically consistent assessment framework that strictly adheres to the principle of “no overlap and no omission”. ② A three-in-one technical pathway of bibliometric screening-correlation verification-practical adaptability optimization was established. The correlation verification focuses on the corresponding relationship between indicators and core characterization factors of river-lake health (e.g., TOC directly reflects organic carbon availability for eutrophication), while the practical adaptability optimization considers the availability of monitoring data in Poyang Lake (e.g., prioritizing commonly monitored indicators such as CODMn, NH3-N, and TOC), avoiding the scientific flaws of subjective weighting and experience-based indicator selection in traditional methods. ③ For the first time, social service function factors such as WRDUR and WFZCI are coupled with natural system indicators. The interaction between indicators is used to characterize the natural–social dual-driving mechanism (e.g., quantifying the negative correlation between WRDUR and EBI-S), providing an operable assessment tool for the accurate diagnosis of Poyang Lake’s health status and multi-factor coordinated regulation (e.g., balancing water resource development and ecological protection). Its methodology can directly support the transformation of water governance from single water quality improvement to natural–social system coordinated optimization.
When applying this optimized assessment indicator system to Poyang Lake’s health assessment, two inherent uncertainties of complex river-lake systems must be addressed: ① Predictive variability of indicator values—significant spatiotemporal variability in the concentrations of water quality indicators such as TP, TN, Chl-a, and TOC due to Poyang Lake’s seasonal hydrological fluctuations, coupled with data gaps for indicators such as Trans and FBLI in some remote coastal areas, may lead to deviations between predicted indicator values and actual conditions. ② Methodological uncertainty in weighting—obvious differences in weight assignment for social service function indicators (e.g., WRDUR) between the Entropy Weight Method (objective weighting) and Analytic Hierarchy Process (AHP, subjective weighting). Such differences in priority ranking may introduce systematic assessment biases. The superposition of these dual uncertainties will directly increase the risk of overestimating Poyang Lake’s actual health status, potentially leading to misallocation of ecological protection resources or insufficient targeting of governance policies.
This study adopts the PINN model and normal distribution assumption to quantitatively identify the above uncertainties, and uses the FOSM method to quantify overestimation risk, so as to obtain more reliable and accurate assessment results.
5. Discussion
5.1. Ecological Interpretation
Total phosphorus (TP), total nitrogen (TN) are key indicators of lake eutrophication, which are easily affected by seasonal hydrological fluctuations, non-point source pollution input and external disturbance, leading to strong spatiotemporal variability and large prediction deviations of indicator values. Meanwhile, the Fish Biological Loss Index (FBLI) is a comprehensive biological indicator closely related to hydrological regime, water quality and habitat integrity, with complex response mechanism and high sensitivity to weight uncertainty. The superposition of indicator value uncertainty and weight uncertainty makes these three indicators show significantly higher overestimation risk than other indicators.
Consistent with most of the previous studies on large freshwater lakes, this study identifies nutrient indicators (TP, TN) as key factors affecting lake health assessment results. Different from traditional deterministic assessment studies that ignore uncertainty, this study further quantifies the overestimation risk driven by dual uncertainties, which is more in line with the actual dynamic characteristics of lake ecosystems. The identification results of high-risk indicators are also supported by relevant studies on Poyang Lake, confirming the reliability of the proposed framework.
The FOSM method is efficient in risk quantification under uncertainty superposition, but it is based on linear approximation assumption. In nonlinear ecological systems with complex interaction mechanisms (such as the nonlinear response of aquatic organisms to nutrient changes), the linear approximation of FOSM may lead to slight deviation in risk calculation results, which is a common limitation of moment-based uncertainty analysis methods in ecological application.
5.2. Core Contributions
Methodological innovation: A standardized dual uncertainty quantification system was developed, integrating PINN-based indicator prediction, game theory-driven weight coordination, and normal distribution-based uncertainty characterization. This addresses the longstanding limitation of insufficient quantification of indicator value and weight uncertainty in existing studies, realizing systematic, operable, and reproducible description of multiple uncertainties in river-lake health assessment. The approach enhances the rigor of uncertainty-informed ecological evaluation by linking historical data dynamics, methodological differences, and statistical distribution characteristics.
Theoretical advancement: A general coupled model of dual uncertainties and overestimation risk was established, clarifying the quantitative relationship between uncertainty sources and overestimation risk. This fills the critical gap in existing research where uncertainty analysis is often isolated (focusing on either indicator values or weights) and lacks a risk coupling mechanism. The derived formulas for single-indicator and comprehensive overestimation risk provide a new theoretical foundation for quantifying evaluation bias, advancing the field from “deterministic assessment” to “probabilistic risk characterization.”
Practical relevance: Validated through a case study of Poyang Lake—a large subtropical seasonal lake with significant ecological and socio-economic value—the framework demonstrates strong adaptability and scalability. It converts abstract uncertainty into actionable risk thresholds and decision-support tools, guiding the rational allocation of ecological protection resources and improving the scientificity of governance decisions. For instance, the identification of TP, TN, and FBLI as high-risk indicators directly informs targeted eutrophication control and biodiversity conservation measures in Poyang Lake, with implications for similar freshwater ecosystems globally.
5.3. Limitations
This study assumes weight uncertainty follows a normal distribution, a simplification of complex real-world conditions where weight variability may be influenced by unmodeled factors (e.g., regional differences in management priorities, data quality variations). Future research could explore more flexible distribution models (e.g., triangular distribution, log-normal distribution) based on larger sample sizes and diverse river-lake types.
While the framework is designed to be general, the case study focuses on Poyang Lake, and its generalizability requires further verification across other ecosystem types (e.g., rivers, reservoirs, arid-region lakes) and climate zones. Differences in hydrological regimes, pollution sources, and ecological characteristics may affect the performance of the framework, necessitating context-specific parameter optimization.
The current framework does not account for the dynamic evolution of uncertainties under external disturbances (e.g., climate change, land-use change, extreme human activities). This limits its applicability in long-term dynamic assessment, where uncertainty sources and their impacts may vary over time.
5.4. Future Directions
Future research should prioritize the following avenues to expand and refine the proposed framework:
Enhance generalizability: Validate the framework across diverse river-lake ecosystems (e.g., alpine lakes, urban rivers, reservoir systems) and climate zones, optimizing model parameters to improve robustness and adaptability to different ecological contexts.
Improve uncertainty characterization: Integrate advanced statistical methods and machine learning techniques (e.g., ensemble learning, Bayesian inference) to refine the characterization of indicator value and weight uncertainty, exploring the impact of non-normal weight distributions on risk quantification results.
Incorporate dynamic disturbances: Integrate dynamic factors (e.g., climate change scenarios, land-use change projections, extreme event frequency) into the uncertainty analysis framework, developing a dynamic risk assessment model to support adaptive management of river-lake ecosystems.
Strengthen policy integration: Establish a closed-loop management mechanism linking risk assessment results to practical governance policies, translating research outcomes into actionable measures (e.g., targeted monitoring plans, adaptive management strategies) to enhance the real-world impact of the framework.
6. Conclusions
This study addresses a critical gap in river-lake health assessment: overestimation risk induced by dual uncertainties (predictive uncertainty of indicator values and methodological uncertainty of weights). A general risk analysis framework integrating uncertainty quantification, weight coordination, and overestimation risk coupling was developed and validated through a case study of Poyang Lake, yielding actionable insights for ecological assessment and governance.
Systematic quantification of dual uncertainties: Leveraging 1264 historical monitoring samples from the Poyang Lake basin, the Physics-Informed Neural Networks (PINN) model was employed to predict indicator values, with historical prediction deviations following a normal distribution to enable quantitative characterization of indicator value uncertainty. For weight uncertainty, four complementary weighting methods—Analytic Hierarchy Process (AHP), Entropy Weight Method (EWM), CRITIC, and Principal Component Analysis (PCA)—generated divergent initial weights, reflecting inherent tradeoffs between expert judgment and data-driven insights. These conflicts were resolved via a game theory-based framework guided by the Nash equilibrium criterion, yielding scientifically balanced coordinated weights. Further assuming a normal distribution (mean = coordinated weight, standard deviation = 1/10 of the mean) achieved standardized quantification of weight uncertainty, with the 3σ rule verifying that random weights strictly fall within the feasible region (0 < weight < 1), ensuring methodological rigor.
Reliable quantification of overestimation risk: The First-Order Second-Moment (FOSM) method—selected for its balance of computational efficiency and accuracy—was adopted to establish a coupled model linking dual uncertainties to overestimation risk. Derived formulas for single-indicator and comprehensive overestimation risk quantified both the probability and magnitude of overestimation risk. Results identified total phosphorus (TP), total nitrogen (TN), and the Fish Biological Loss Index (FBLI) as high-risk indicators in Poyang Lake, with maximum allowable thresholds ranging from 5.10 to 7.40, 7.04 to 9.38, and 6.05 to 9.02, respectively, across risk levels of 1–50%. These indicators are most prone to overestimation and require prioritized monitoring and management.
Practical applicability of the framework: The comprehensive overestimation risk score for Poyang Lake ranged from 74.16 to 81.17 across the defined risk interval, providing clear thresholds for ecological governance. The color-coded risk histogram intuitively visualized high-risk indicators and their deviation magnitudes, offering targeted support for optimizing the assessment indicator system and adjusting governance priorities—such as strengthening eutrophication control and ecological baseflow protection. Notably, the framework’s modular design ensures its transferability to other river-lake ecosystems beyond Poyang Lake.