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Open AccessArticle

The Utility of Dreissena polymorpha for Assessing the Viral Contamination of Rivers by Measuring the Accumulation of F-Specific RNA Bacteriophages

1
Laboratoire de Chimie Physique et Microbiologie pour l’Environnement, UMR 7564, Institut Jean Barriol, Faculté des Sciences et Technologies, Université de Lorraine, LCPME, F-54506 Vandœuvre-lès-Nancy, France
2
Unité Stress Environnementaux et Biosurveillance des Milieux Aquatiques, UMR-I 02 UFR, Sciences Exactes et Naturelles, Moulin de la Housse, BP 1039, Université Reims Champagne Ardenne, SEBIO, 51687 Reims, France
3
Food Safety Department, Actalia, F-50000 Saint-Lô, France
*
Author to whom correspondence should be addressed.
Academic Editor: Françoise S. LUCAS
Water 2021, 13(7), 904; https://doi.org/10.3390/w13070904
Received: 10 February 2021 / Revised: 20 March 2021 / Accepted: 23 March 2021 / Published: 26 March 2021
River water that receives treated wastewater can be contaminated by pathogens including enteric viruses due to fecal pollution, which may represent an important public health hazard. There is a great diversity of enteric viruses and fecal bacteriophages, especially F-specific RNA bacteriophages (FRNAPHs), are commonly proposed as indicators of viral pollution due to a variety of characteristics such as their structural similarities to the main enteric viruses, their high concentrations in raw wastewater and their environmental survival rate, which is better than other cultivable enteric viruses. However, evaluating the viral contamination of water on the basis of FRNAPH concentration levels continues to present a challenge. This is because the quality of detection is strongly dependent on the quantity of viral particles, high spatio-temporal variabilities and the physico-chemical conditions of the water during sampling. To overcome these limitations, the present study aims to evaluate whether the bivalve mollusk Dreissena polymorpha (zebra mussel) could be considered a suitable experimental model for assessing the viral contamination of rivers. In order to determine this, the capacity of D. polymorpha to accumulate FRNAPHs and assimilate them into their soft tissue was studied. This provided a proof of concept for the use of D. polymorpha to evaluate the viral contamination of surface water. Two experiments were conducted: (1) an in situ experiment to confirm that zebra mussels naturally accumulated FRNAPHs and (2) a laboratory experiment to determine the accumulation and depuration kinetics of FRNAPHs in D. polymorpha tissue. The study highlights the capacity of the mussels to accumulate infectious FRNAPHs both on a laboratory scale under controlled conditions as well as in situ at different sites that are representative of different bodies of water. An analysis of the mussels’ soft tissue showed that they were capable of reflecting the water’s contamination level very quickly (within less than 24 h). Moreover, the soft tissue retained the viral load much longer than the water due to a low depuration rate. The analysis of FRNAPH concentrations in mussels exposed in situ suggested that there were differences in contamination levels between sites. These preliminary results underline the potential utility of zebra mussels in assessing viral contamination by measuring the accumulation of FRNAPHs in their tissue. This may ultimately enable stakeholders to use zebra mussels as a means of monitoring viral pollution in surface water. View Full-Text
Keywords: bacteriophage; surface water; mollusk; bioaccumulation; biomonitoring bacteriophage; surface water; mollusk; bioaccumulation; biomonitoring
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MDPI and ACS Style

Capizzi-Banas, S.; Palos Ladeiro, M.; Bastien, F.; Bonnard, I.; Boudaud, N.; Gantzer, C.; Geffard, A. The Utility of Dreissena polymorpha for Assessing the Viral Contamination of Rivers by Measuring the Accumulation of F-Specific RNA Bacteriophages. Water 2021, 13, 904. https://doi.org/10.3390/w13070904

AMA Style

Capizzi-Banas S, Palos Ladeiro M, Bastien F, Bonnard I, Boudaud N, Gantzer C, Geffard A. The Utility of Dreissena polymorpha for Assessing the Viral Contamination of Rivers by Measuring the Accumulation of F-Specific RNA Bacteriophages. Water. 2021; 13(7):904. https://doi.org/10.3390/w13070904

Chicago/Turabian Style

Capizzi-Banas, Sandrine; Palos Ladeiro, Mélissa; Bastien, Fanny; Bonnard, Isabelle; Boudaud, Nicolas; Gantzer, Christophe; Geffard, Alain. 2021. "The Utility of Dreissena polymorpha for Assessing the Viral Contamination of Rivers by Measuring the Accumulation of F-Specific RNA Bacteriophages" Water 13, no. 7: 904. https://doi.org/10.3390/w13070904

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