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Article

Mengovirus as Process Control Virus in the Monitoring of Genomic RNA and Infectivity of Enteric Viruses in Water Matrices

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Instituto de Saúde Ambiental, Faculdade de Medicina da Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisboa, Portugal
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Direção de Laboratórios (LAB), Empresa Portuguesa das Águas Livres (EPAL), Avenida de Berlim, 15, 1800-031 Lisboa, Portugal
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Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa & CESAM—Centro de Estudos do Ambiente e do Mar, Universidade de Aveiro, 3810-193 Aveiro, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: Athena Mavridou
Water 2021, 13(20), 2834; https://doi.org/10.3390/w13202834
Received: 31 August 2021 / Revised: 1 October 2021 / Accepted: 7 October 2021 / Published: 12 October 2021
(This article belongs to the Section Water and One Health)
The present study, developed in the scope of a survey to monitor enteric viruses in natural surface water and drinking water sources, addressed the suitability of mengovirus to assess viral recovery rates at two steps of the water sampling process. In a pilot campaign comprising two samples from each type of water source, when mengovirus was added after the filtration/primary concentration step, the recovery rates of viral RNA were higher than 18% and identical for both water matrices. In a one-year sampling campaign, where mengovirus was present along the whole sample processing (addition in the filtration/primary concentration step), significantly different recovery rates were observed between water matrices: usually higher than 1% in drinking water and under 1% in surface water. The results suggest the first stage of the water sampling process and the type of water matrix are the most influential factors for viral RNA recovery. This study also addressed and evidenced mengovirus replication and titration in Vero E6 cultures and showed infectious mengovirus to be recovered from samples of both types of water matrix. These results anticipate a more comprehensive applicability of mengovirus as a process control virus in the monitoring of viruses in water, extended to viral infectivity. View Full-Text
Keywords: drinking water; enteric viruses; environmental monitoring; method 1615; RT-qPCR; surface water; viral infectivity; virus titration; water quality; water treatment drinking water; enteric viruses; environmental monitoring; method 1615; RT-qPCR; surface water; viral infectivity; virus titration; water quality; water treatment
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MDPI and ACS Style

Salvador, D.; Neto, C.; Carneiro, R.N.; Caeiro, M.F. Mengovirus as Process Control Virus in the Monitoring of Genomic RNA and Infectivity of Enteric Viruses in Water Matrices. Water 2021, 13, 2834. https://doi.org/10.3390/w13202834

AMA Style

Salvador D, Neto C, Carneiro RN, Caeiro MF. Mengovirus as Process Control Virus in the Monitoring of Genomic RNA and Infectivity of Enteric Viruses in Water Matrices. Water. 2021; 13(20):2834. https://doi.org/10.3390/w13202834

Chicago/Turabian Style

Salvador, Daniel, Célia Neto, Rui Neves Carneiro, and Maria Filomena Caeiro. 2021. "Mengovirus as Process Control Virus in the Monitoring of Genomic RNA and Infectivity of Enteric Viruses in Water Matrices" Water 13, no. 20: 2834. https://doi.org/10.3390/w13202834

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