Next Article in Journal
Exploring the Spatial Impact of Green Infrastructure on Urban Drainage Resilience
Previous Article in Journal
Microbial Biofilm Diversity and Prevalence of Antibiotic Resistance Genes in Drinking Water Distribution System of Peshawar, Pakistan
Article

Facile Detection of Vitamin B12 with Copper Oxide Nanocrystal Graphenic Composite Electrode

1
School of Civil Engineering Architecture and Environment, Hubei University of Technology, Wuhan 430068, China
2
School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan 430068, China
3
School of Bioengineering and Food Science, Hubei University of Technology, Wuhan 430068, China
*
Author to whom correspondence should be addressed.
Water 2021, 13(13), 1790; https://doi.org/10.3390/w13131790
Received: 24 May 2021 / Revised: 23 June 2021 / Accepted: 26 June 2021 / Published: 28 June 2021
Vitamin B12 (VB12) is applied as the cofactors in various important enzymatic reactions and is involved in gene expression regulation mediated by B12-riboswitch and the VB12-dependent photoreceptor. Rapid detection VB12 concertation in a given environment may provide insights in the evaluation of micronutrient levels and the physiological and ecological performances of organisms under the relevant condition. This study demonstrating an amperometric approach to quantify the VB12 in biological samples without complicated sample pretreatment. The electrochemical oxidation step was conducted with a plain graphenic electrode to convert all nitrogen groups within the VB12 molecules to NO3 at 1.3 V vs. Ag/AgCl for 15 min. VB12 was quantified stoichiometrically according to the oxidized nitrate anions, which were reduced with copper oxide nanocrystal decorated graphenic electrode. Cathodic polarization was conducted with a graphite rod electrode before nitrate reduction to eliminate the potential interferences. Under optimized experimental conditions, the presented approach gave a wide detection linear range of 0.15–7378 nmol L−1 and the detection limit was 0.59 nmol L−1. The results for biological samples were comparable to those of the HPLC method. These results indicated that successively combined anodic and cathodic polarization enhanced the detection sensitivity and efficiency of the electrode towards VB12. The proposed electrode shows potential in terms of efficiency, reliability and accuracy for rapid determination of VB12 in biological samples. View Full-Text
Keywords: copper oxide; electrochemical detection; GUITAR; electrochemical oxidation; vitamin B12 copper oxide; electrochemical detection; GUITAR; electrochemical oxidation; vitamin B12
Show Figures

Figure 1

MDPI and ACS Style

Tian, C.; Zhao, N.; Jiang, X.; Wan, D.; Xie, Y. Facile Detection of Vitamin B12 with Copper Oxide Nanocrystal Graphenic Composite Electrode. Water 2021, 13, 1790. https://doi.org/10.3390/w13131790

AMA Style

Tian C, Zhao N, Jiang X, Wan D, Xie Y. Facile Detection of Vitamin B12 with Copper Oxide Nanocrystal Graphenic Composite Electrode. Water. 2021; 13(13):1790. https://doi.org/10.3390/w13131790

Chicago/Turabian Style

Tian, Chao, Na Zhao, Xinyue Jiang, Duanji Wan, and Yuqun Xie. 2021. "Facile Detection of Vitamin B12 with Copper Oxide Nanocrystal Graphenic Composite Electrode" Water 13, no. 13: 1790. https://doi.org/10.3390/w13131790

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop