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Article

Effects of Plastic Debris on the Biofilm Bacterial Communities in Lake Water

1
Chengdu Engineering Corporation Limited, Power China, Chengdu 610072, China
2
College of Environment, Hohai University, Nanjing 210098, China
3
School of Environment, Nanjing Normal University, Nanjing 210023, China
4
State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210098, China
5
Department of Geography and Environmental Science, University of Reading, Whiteknights, Reading RG6 6AB, UK
*
Authors to whom correspondence should be addressed.
Academic Editor: Adriano Sfriso
Water 2021, 13(11), 1465; https://doi.org/10.3390/w13111465
Received: 18 March 2021 / Revised: 15 April 2021 / Accepted: 18 May 2021 / Published: 23 May 2021
Increasing discharge of plastic debris into aquatic ecosystems and the worsening ecological risks have received growing attention. Once released, plastic debris could serve as a new substrate for microbes in waters. The complex relationship between plastics and biofilms has aroused great interest. To confirm the hypothesis that the presence of plastic in water affects the composition of biofilm in natural state, in situ biofilm culture experiments were conducted in a lake for 40 days. The diversity of biofilm attached on natural (cobble stones (CS) and wood) and plastic substrates (Polyethylene terephthalate (PET) and Polymethyl methacrylate (PMMA)) were compared, and the community structure and composition were also analyzed. Results from high-throughput sequencing of 16S rRNA showed that the diversity and species richness of biofilm bacterial communities on natural substrate (observed species of 1353~1945, Simpson index of 0.977~0.989 and Shannon–Wiener diversity index of 7.42~8.60) were much higher than those on plastic substrates (observed species of 900~1146, Simpson index of 0.914~0.975 and Shannon–Wiener diversity index of 5.47~6.99). The NMDS analyses were used to confirm the taxonomic significance between different samples, and Anosim (p = 0.001, R = 0.892) and Adonis (p = 0.001, R = 808, F = 11.19) demonstrated that this classification was statistically rigorous. Different dominant bacterial communities were found on plastic and natural substrates. Alphaproteobacterial, Betaproteobacteria and Synechococcophycideae dominated on the plastic substrate, while Gammaproteobacteria, Phycisphaerae and Planctomycetia played the main role on the natural substrates. The bacterial community structure of the two substrates also showed significant difference which is consistent with previous studies using other polymer types. Our results shed light on the fact that plastic debris can serve as a new habitat for biofilm colonization, unlike natural substrates, pathogens and plastic-degrading microorganisms selectively attached to plastic substrates, which affected the bacterial community structure and composition in aquatic environment. This study provided a new insight into understanding the potential impacts of plastics serving as a new habitat for microbial communities in freshwater environments. Future research should focus on the potential impacts of plastic-attached biofilms in various aquatic environments and the whole life cycle of plastics (i.e., from plastic fragments to microplastics) and also microbial flock characteristics using microbial plastics in the natural environment should also be addressed. View Full-Text
Keywords: biofilm; plastic debris; high-throughput sequencing; biodiversity; community structure biofilm; plastic debris; high-throughput sequencing; biodiversity; community structure
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MDPI and ACS Style

Shen, C.; Huang, L.; Xie, G.; Wang, Y.; Ma, Z.; Yao, Y.; Yang, H. Effects of Plastic Debris on the Biofilm Bacterial Communities in Lake Water. Water 2021, 13, 1465. https://doi.org/10.3390/w13111465

AMA Style

Shen C, Huang L, Xie G, Wang Y, Ma Z, Yao Y, Yang H. Effects of Plastic Debris on the Biofilm Bacterial Communities in Lake Water. Water. 2021; 13(11):1465. https://doi.org/10.3390/w13111465

Chicago/Turabian Style

Shen, Chao, Liuyan Huang, Guangwu Xie, Yulai Wang, Zongkai Ma, Yu Yao, and Hong Yang. 2021. "Effects of Plastic Debris on the Biofilm Bacterial Communities in Lake Water" Water 13, no. 11: 1465. https://doi.org/10.3390/w13111465

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