Next Article in Journal
Multi-Site Statistical Downscaling Method Using GCM-Based Monthly Data for Daily Precipitation Generation
Previous Article in Journal
Pipeline Scour Rates Prediction-Based Model Utilizing a Multilayer Perceptron-Colliding Body Algorithm
Open AccessArticle

Detection of Cyanotoxin-Producing Genes in a Eutrophic Reservoir (Billings Reservoir, São Paulo, Brazil)

1
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Rua Botucatu, 862, São Paulo 04023-901, Brazil
2
Departamento de Ciências Ambientais, Universidade Federal de São Paulo, Rua Prof. Artur Riedel, 275, Diadema 09972-270, Brazil
3
Centro de Pesquisa em Plantas Avasculares e Fungos, Núcleo de Pesquisa em Ficologia, Instituto de Botânica, Av. Miguel Estéfano, 3687, São Paulo 04301-012, Brazil
*
Author to whom correspondence should be addressed.
Water 2020, 12(3), 903; https://doi.org/10.3390/w12030903
Received: 2 October 2019 / Revised: 5 December 2019 / Accepted: 13 December 2019 / Published: 23 March 2020
(This article belongs to the Section Water Quality and Ecosystems)
CyanoHABs (cyanobacterial harmful algal blooms) are blooms of cyanobacteria capable of producing cyanotoxins, a large group of secondary metabolites that are toxic to most eukaryotes. In this work, the main aim was to evaluate the presence of multiple genes from each of the clusters responsible for biosynthesis of cyanotoxins (cylindrospermopsin, microcystin and saxitoxin) in total DNA obtained from sixteen environmental water samples by PCR. Microcystin gene mcyE was amplified in all analyzed samples. Among the cylindrospermopsin genes analyzed, only the cyrC gene was amplified from DNA obtained from three of sixteen samples. Of the three different saxitoxin genes analyzed, sxtB and sxtI were present in four and three of the sixteen samples studied, respectively, and sxtA did not show any positive result. Based on our results, we suggest caution when using only one gene from the full clusters responsible for biosynthesis of cyanotoxins, given that it may not be sufficient to confirm or exclude the toxigenic potential of a sample. View Full-Text
Keywords: cyanotoxin genes; environmental monitoring; PCR cyanotoxin genes; environmental monitoring; PCR
Show Figures

Figure 1

MDPI and ACS Style

Ribeiro, M.S.F.; Tucci, A.; Matarazzo, M.P.; Viana-Niero, C.; Nordi, C.S.F. Detection of Cyanotoxin-Producing Genes in a Eutrophic Reservoir (Billings Reservoir, São Paulo, Brazil). Water 2020, 12, 903.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop