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Review

Membrane-Associated Proteins in Giardia lamblia

by
María C. Touz
*,
Constanza Feliziani
and
Andrea S. Rópolo
*
Instituto de Investigación Médica Mercedes y Martín Ferreyra—INIMEC-CONICET—Universidad Nacional de Córdoba, Friuli 2434, Córdoba 5000, Argentina
*
Authors to whom correspondence should be addressed.
Genes 2018, 9(8), 404; https://doi.org/10.3390/genes9080404
Submission received: 17 July 2018 / Revised: 3 August 2018 / Accepted: 7 August 2018 / Published: 10 August 2018
(This article belongs to the Special Issue Membrane Proteins in Parasitic Protozoa)
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Abstract

:
The manner in which membrane-associated proteins interact with the membrane defines their subcellular fate and function. This interaction relies on the characteristics of the proteins, their journey after synthesis, and their interaction with other proteins or enzymes. Understanding these properties may help to define the function of a protein and also the role of an organelle. In the case of microorganisms like protozoa parasites, it may help to understand singular features that will eventually lead to the design of parasite-specific drugs. The protozoa parasite Giardia lamblia is an example of a widespread parasite that has been infecting humans and animals from ancestral times, adjusting itself to the changes of the environment inside and outside the host. Several membrane-associated proteins have been posted in the genome database GiardiaDB, although only a few of them have been characterized. This review discusses the data regarding membrane-associated proteins in relationship with lipids and specific organelles and their implication in the discovery of anti-giardial therapies.

Graphical Abstract

1. Introduction

The genus Giardia constitutes a group of unicellular eukaryotic organisms within the large group Excavata, which is characterized by its morphological simplicity [1]. It has been hypothesized that the absence of complexity in this group is a consequence of two fundamental conditions: the early separation of the eukaryotic trunk and the adaptation to parasitism through a process of reductive evolution [2,3]. Still, the evolutionary mechanisms behind the cellular characteristics of these organisms are unknown.
Giardia lamblia (syn. Giardia intestinalis, Giardia duodenalis) is a unicellular eukaryotic parasite that has attracted scientific attention not only for its worldwide dissemination but also for its particular endomembrane system. The vegetative form of the parasite, the trophozoite, contains two nuclei surrounded by their respective nuclear membranes (NMs), which are in close contact with the endoplasmic reticulum (ER) that covers bilaterally almost the whole body of the parasite [4]. It also contains mitosomes, highly adapted forms of mitochondria, which are surrounded by two membranes, do not contain DNA, and do not produce ATP, but participate in the formation of iron–sulfur clusters [5].
Besides the NMs, the ER, and the mitosomes, G. lamblia possesses peripheral vacuoles (PVs), which are located below the plasma membrane (PM) of the trophozoites and function as both endosomes and lysosomes [6]. The maintenance of the homeostasis of these organelles during their life cycle is crucial for the survival of the parasite and implies a series of coordinated events in which protein trafficking is central.
Even with an endomembrane system reduced to the NMs, ER, and PVs, the selective sorting and trafficking of membrane proteins takes place in this organism, although with some particularities. Although the membranes of intracellular organelles are formed by the semipermeable barrier of the lipid bilayer, the properties of a particular membrane cannot be attributed only to the lipid composition. Rather, the type of proteins associated with the lipid layer will delineate the function of a biological membrane. In most eukaryotic organisms, non-cytosolic proteins and lipids are synthesized in the ER and reach the target organelle once they have been selected and directed from the Golgi apparatus. In G. lamblia, instead, proteins with a signal peptide are directly sorted from ER exit sites to the PVs or to the PM, while lipids are rarely synthesized de novo but are obtained from the small intestine environment [7,8]. Moreover, an unusual de novo synthesis of secretory vesicles occurs only during the differentiation of the G. lamblia trophozoite into cyst, which involves a series of protein synthesis and membrane arrangement events [7].
How proteins and lipids are transported, recycled, and modified is still a matter of study, but it is clear that advances in this field will help us to understand the similarities as well as the differences between G. lamblia and its host and aid us in the discovery of new specific targeted drugs. This review discusses the data regarding membrane-associated proteins that have been experimentally analyzed, in relationship with lipids and specific organelles and their implication in the discovery of anti-giardial therapies.

2. Fat Attachment: Phosphoinositide-Binding Proteins

The analysis of trophozoite membranes showed that they contain at least two species of phosphatidylinositol (PI) [9], but there is no published data so far regarding the direct presence of phosphoinositides (PIPs), which are the temporal and spatial regulators of membrane trafficking and cell signalling [10,11]. Nevertheless, the expression of two PI3-kinases and one PI4-kinase has been reported, supporting the existence of modified phosphatidylinositol [12,13]. Besides, several proteins containing PIP-binding domains have been characterized in G. lamblia, favoring the existence of PIPs and suggesting that there may be a combination of organelle–membrane selectivity and protein function.
The FYVE (Fab1p, YOTB, Vac1p, and EEA1) domain: In 2010, Sinha et al. reported a protein (GL50803_16653) containing a conserved FYVE domain, which is expressed during the cell cycle of G. lamblia [14]. It has been reported that proteins containing the FYVE domain are able to bind to PI3P located in the endosomal and lysosomal compartments in mammalian and yeast cells [15], and analysis of lipid binding using the Protein Lipid Overlay (PLO) assay showed that the G. lamblia FYVE domain alone possesses higher binding affinity for PI3P than for PIs and PI4P [14]. The expression of the fusion of green fluorescent protein (GFP) and the giardial FYVE domain in yeast showed that it localizes to endosomes [14]. Considering that PI3P is present in endosomes, PI4P is in the Golgi, and PIs are distributed in the ER and vacuoles in yeast cells, it is possible that GL50803_16653 interacts in G. lamblia with the PV membranes, the all-in-one endosomal–lysosomal compartment in this parasite. In line with this, since the FYVE domain is present in Vps27, an ESCRT-0 complex subunit that initiates the multivesicular body (MVB) sorting pathway at the endosomal membranes in other cells via its binding to PI3P at the membrane, it is likely that the function of GL50803_16653 is related to sorting to the PVs. However, no indication of PI3P enrichment has been described so far in the PVs in G. lamblia, making the function of GL50803_16653 uncertain.
Epsin N-terminal homology (ENTH) domain: The ENTH is an evolutionarily conserved domain described primarily in the ENTH/ANTH/VHS protein superfamily that participates in clathrin-mediated endocytosis. The ENTH domain defines monomeric adaptor proteins described as epsin or epsin-related (epsinR), which have the ability to interact with PI4,5P2 at the plasma membrane or with PI4P at the Golgi membrane, respectively, in mammalian and yeast cells [16,17]. Recently, we described the function of the monomeric adaptor protein in G. lamblia (GL50803_3256), termed GlENTHp (for G. lamblia ENTH protein), the human influenza hemagglutinin (HA)-tagged native version of which was able to bind to PI3,4,5P3 and PI4P, while the HA-tagged ENTH domain alone bound to PI3,4,5P3 in PLO assays [18,19]. This unique protein fulfils a dual function, as a classical epsin protein in receptor-mediated endocytosis (RME) via the tetrameric clathrin-adaptor AP-2, clathrin, and ubiquitin, and like epsinR as a critical factor in ER-to-PVs trafficking of proteins, acting in concert with AP-1 adaptors [18].
The PX domain: Proteins containing the phagocyte NADPH oxidase phox-(PX) domain have been described in many cell types and have been implicated in vesicular trafficking, cell signaling, and protein sorting, among other functions [20,21]. Like the FYVE, the PX domains interact with PI3P but may also bind to other PIs [22,23]. They have a preference for the endosomal compartments, although they can also be found in other membranes [24]. In G. lamblia, six different PX domains containing proteins have been described on the basis of an in silico searching of the GiardiaDB (http://giardiadb.org/giardiadb/), Assemblage A isolate WB GL50803) [25]. The PX domains of these proteins (GL50803_16596, GL50803_42357, GL50803_16548, GL50803_24488, GL50803_7723, and GL50803_16595) contain the characteristic canonical PX domains, and all but two include basic amino acids, essential for binding the negatively charged PIP head group. PIP binding of their PX domain, cellular localization, and protein expression were determined for these proteins and are listed in Table 1. Although most of the PX-containing domain proteins possess other additional domains, the in silico analysis of the sequence of these G. lamblia proteins did not show other special sequences [25]. However, the partial characterization of these PX domain-only proteins in G. lamblia might be associated with their role in protein trafficking. For instance, sorting nexins (SNX), proteins containing a PX-domain that binds to PI3P and other PIPs enriched in endosomal membranes [26], have been suggested as part of the retromer complex [27]. These SNXs contain an additional BAR (Bin, amphiphysin, Rvs) motif, which mediates the recruitment of the retromer complex to the endosomal membrane, driving tubule formation [28]. Nevertheless, the participation of non-BAR SNX3 has also been described as mediating the recruitment of the cargo-selective retromer complex [29]. Two proteins, GL50803_24488 and GL50803_16548, described by Jana et al., 2017 [24], possess high sequence similarity to the non-BAR SNX proteins, SNX15 and SNX17, respectively [30,31,32]. The data obtained so far suggest that GL50803_24488 and GL50803_16548 participate in the recovery of membrane proteins from the PVs, where they have the capacity to bind to PI3P and the cargo subunit of the retromer to drive non-tubular transport to the ER in G. lamblia [30,31,32].
Another example of PX domain-containing proteins is the t-SNARE Vam7p, a yeast t-SNARE localized to the vacuole [33]. Vamp7p participates in the docking and fusion of specific vesicles to the lysosomal vacuole, acting as a SNAP-25 homologue [33]. In G. lamblia, 12 SNARE-like proteins have been found [34], but none of them contains a PX domain. Since non-SNAP-25 family proteins are present in the G. lamblia genome, it is possible that, in contrast to Vamp7p, PI3P-specific PX domains of the SNAREs are not required for appropriate PV protein localization.
Except for GlENTHp, the functional properties of PIP-binding proteins have not been analyzed. In the case of GlENTHp, it was found that a reduction of its expression caused a defect in G. lamblia cell growth, probably associated with the alteration of the parasite nutrition and PVs homeostasis [18]. These results and those showing that PIP-associated proteins are related to PV structure and function have expanded the knowledge of the critical role of PVs in G. lamblia cell survival and may help in the design of new therapeutic parasiticide molecules.

3. Take Me Home: Integral Protein Targeting

Lysosomes play a central function not only in degrading biomacromolecules but also in nutrient and pathogen sensing and membrane repair [35]. Moreover, it was demonstrated that some secretory cell lysosomes, called secretory lysosomes or lysosome-related organelles (LROs), contain proteins destined for secretion in addition to the lysosomal hydrolases [36]. These dual-function organelles are unusual in that they serve both as a degradative and as a secretory compartment. Similarly, G. lamblia possesses PVs that function not only as endo-lysosomal compartments but probably also as sorting stations [37,38]. Besides these particularities, protein sorting and delivery to the PVs seem to follow canonical routes described in other cells. For instance, it is well known that lysosomal integral membrane proteins (e.g., LAMP/LIMP family proteins) are transported from the Golgi apparatus to lysosomes by binding their cytosolic motifs to AP complexes [39]. The μ subunits of AP-1, AP-2, and AP-3 bind directly to YXXØ-type sequences, while the γσ1, ασ2, and δσ3 hemicomplexes bind to the [DE]XXXL[LI] sequences [40,41]. In G. lamblia, we reported that a type I-membrane cysteine protease (GL50803_14566), termed ESCP (encystation-specific cysteine protease), is transported to the PVs through the tyrosine-based YRPI motif located at its cytoplasmic tail [42]. The YRPI is essential for AP-1 binding and transport from the ER exit site to the PVs in growing trophozoites [42,43]. Also, similar to the mannose 6-phosphate receptors (MPRs) (and Vps10p in yeast), in which the trafficking of the MPR-hydrolase complex to endosomes depends on sorting signals present in the receptor cytosolic tails [44], Vps receptor (GL50803_28954) delivery to the PVs in G. lamblia depends on a YQII (YXXØ-type) motif present in its cytosolic tail [45]. This motif is essential for AP-1 interaction and trafficking of the soluble acid phosphatase to the PVs, where it functions as a degradative enzyme [45].
Another known type I-membrane protein that cycles between the PM of trophozoites and the PVs throughout a tyrosine-based motif in G. lamblia is GlLRP (Giardia Low-density lipoprotein Receptor-related Protein, GL50803_113565) [46]. GlLRP shares the substrate-N-terminal binding domain and a FXNPXY-type endocytic motif with human LRP1 and internalizes both low-density lipoproteins (LDL) and chylomicrons, as shown by in vitro studies [46]. The FXNPXY motif of GlLRP was shown to bind directly to the μ2 subunit of AP-2, this being necessary for its proper localization, processing, and function.
A quick analysis of the proteins described reveals that the trafficking of integral membrane proteins to specific organelles depends on particular motifs in this parasite. However, this may be true only for integral membrane proteins delivered to the PVs. For example, the mitosomal GiMOMP35 protein contains two transmembrane domains in its N-terminal region and is embedded in the outer mitosomal membrane [47]. The targeting of this membrane protein does not rely on a sorting signal composed of a sequence of amino acids but rather on the presence of both transmembrane domains [47].
Similar to GlENTHp, alteration (in the expression, transport, etc.) of any of the integral membrane proteins that travel to the PVs causes a defect either in cell growth or in the differentiation to the resistant cyst form [18,37,38,42,43]. This are further data supporting the usefulness of altering PV function as a way of controlling parasite growth and dissemination.

4. The Ultimate Fate: Default Targeting to the Plasma Membrane

The trafficking of integral membrane proteins to the plasma membrane has been extensively studied for the variant-specific surface proteins (VSPs), including the specific domains involved in their transport [48,49]. However, there are other integral proteins, like the type I High Cysteine Non-variant Cyst protein (HCNCp) or the DHHC-CDR proteins, for which, although their localization has been defined in a particular membrane, the transport pathway or the domains involved in their trafficking remain unexplored.
The VSPs: Variant-specific surface proteins are a family of proteins that cover the entire surface of the parasite, including the flagella. They are cysteine-rich proteins with particular features: many CXXC motifs, one or two GGCY motifs, zinc finger motifs, a conserved transmembrane region, and an invariable C-terminal tail composed of only five amino acids: CRGKA [50,51,52,53,54]. Each protein contains a highly variable N-terminal region that is exposed to the extracellular media and has been associated with the evasion of host immune response by a mechanism called antigenic variation [55,56,57,58]. The C-terminal regions of these proteins, containing the transmembrane portion and the invariable cytoplasmic tail, have been proposed to be involved in protein trafficking to the parasite membrane [59,60]. In addition, there is evidence that indicates that posttranslational modifications, like protein palmitoylation and citrullination of the C-terminal tail, are directly linked to the process of antigenic variation [48,61] (Figure 1).
The occurrence of palmitoylation in VSPs was described around 20 years ago and, considering the in vitro experiments indicating that the palmitoylation site was located within the conserved putative transmembrane domain of these proteins, the authors suggested that this fatty acid modification may be involved in anchoring the VSP to the plasma membrane [60,62,63]. This conserved region was suggested as a sorting motif to the plasma membrane. However, some years later, it was demonstrated that neither the amino acid composition of the transmembrane domain nor the C-terminal CRGKA tail were involved in the traffic to the plasma membrane [48]. More recently, an exhaustive study was carried out using mutated VSPs and analyzing their localization, that identified two essential motifs responsible for membrane localization. One of them is located close to the C-terminus and has two CXXCs motifs separated by 12 to 15 amino acid residues, while the other motif is hydrophobic and is also located in the C-terminus [49]. Also, these authors confirmed that neither the C-terminal CRGKA motif nor the N-terminal region are necessary for VSP localization [49].
In contrast with these results, there is another report showing that a chimera, containing the N-terminal leader sequence of a G. lamblia cyst wall protein followed by the Toxoplasma gondii SAG1 exodomain and the VSPH7 transmembrane domain, is directed to the plasma membrane of trophozoites only if the CRGKA cytoplasmic tail is added [60]. This report, rather than showing the critical role of the CRGKA tail in VSP protein trafficking to the plasma membrane, demonstrates the need to use the correct tools to study how membrane-associated proteins are delivered and integrated into a specific organelle. At least for VSP trafficking, it remains clear that neither the exchange of the transmembrane domain of the VSPs with another sequence similar in length nor the absence of the CRGKA tail alter the fate of VSPs in the plasma membrane of trophozoites, leading to other interesting questions, such as how these two conserved domains participate in the regulation and preservation of antigenic variation in this parasite. For the studies on VSP structure, localization, and function, three VSPs were mainly used: The VSPH7 (GSB_150963) [52], the VSP9B10 or VSP-88 (GL50803_101074) [64], and the VSP/1267 (GL50803_112208) [50].
The protein HCNCp (HCNCp, GL50803_40376) was originally described as a VSP-like protein, considering its membrane localization and the presence of multiple CXXC and GGCY motifs. However, it lacks the zinc (Zn) finger motifs present in all VSPs, and both the conserved C-terminal transmembrane region and the short cytoplasmic tail are divergent from those of VSPs [65]. In vegetative growing cells, this protein localizes to the nuclear envelope and the nuclei, but during the process of cell differentiation into cysts, it was found inside the encystation-specific vesicles (ESVs), colocalizing with the cyst proteins during their trafficking to form the cyst wall. At the end of the encystation process, HCNCp localizes to the wall and cell body of cysts [65]. A clear difference between VSPs and HCNCp trafficking is that, while VSPs follow a direct pathway from the ER to the plasma membrane during the entire cell life cycle, HCNCp requires distinct secretory pathways for growth and differentiation, suggesting a significant role of HCNCp in cyst wall formation. So far, the particular function of each region within the HCNCp protein is not known, but it was recently observed that this protein is palmitoylated, although the palmitoylation site and the functional significance of this modification remain unknown [66].
The DHHC-CDR proteins: These are a family of polytopic integral membrane proteins containing the conserved Asp-His-His-Cys cysteine-rich (DHHC-CDR) domain required for palmitoyl acyltransferase (PAT) catalytic activity in eukaryotic cells [67,68,69]. The PATs possess three to six transmembrane domains, with the C- and N-terminus facing the cytoplasm. In G. lamblia, 9 DHHC-like proteins were identified [66]. The analysis of a group of DHHC proteins showed that three of them localize in the ER and the nuclear envelope, which correlates with the localization observed for HCNCp (nuclear envelope), during growth. Moreover, a partial co-localization of these enzymes with a cyst wall protein suggests that they may be involved in the process of encystation [66]. On the other hand, it was demonstrated that the DHHC GL50803_42184 localizes to the plasma membrane and is involved in the palmitoylation of VSPs [48]. However, there is another DHHC-CDR (GL50803_8711) that localizes to the plasma membrane, but its substrates as well as the domains involved in its localization remain to be elucidated.
Other integral membrane proteins are described in the GiardiaDB, and some of them have been found by proteomic analysis at different stages of the parasite cell cycle [70,71,72]. These findings and others that allow a more detailed description of the integral membrane proteins in G. lamblia will open the doors to a better understanding of these proteins and their role in survival and virulence.

5. Conclusions and Future Directions

Complete genomes are available in the GiardiaDB for assemblages A, B, and E, and comparative analyses of these can now be made. Genetic studies and genome sequencing have laid an important foundation for understanding G. lamblia. This approach is used to dissect species of the genus Giardia and has shown that these assemblages are very diverse, presenting genetic variation between isolates, which appear to possess sufficient differences to be considered distinct [73,74]. Most of the biochemical and cell biological studies have been made using different isolates from the assemblage A (WB1267, C6, etc.), because fusion proteins can be expressed from either episomal or integrated vectors in these trophozoites [75]. There is only one report of integrated transfection of assemblage B (isolate GSH7) [76] and no evidence of transfection of assemblage E exists so far. In Table 2, we depict annotated proteins that show a high degree of sequence similarity between assembles, compared with the ones described in this review. This data might help to perform comparative analyses regarding the differences in infectivity, virulence, and specificity of each assemblage.
Compared with what was reported in other organisms, there are limited studies regarding the role of membrane-associated proteins (besides VSPs) in G. lamblia (Figure 2). In fact, most of the findings have been collateral to the analysis of diverse mechanisms associated with the parasite’s survival. However, emerging results sustain a critical role of membrane-associated proteins in the physiology of the parasite and disclose the role of proteins and lipids in the control of antigenic variation and cell differentiation into resistant forms. This may lead to the discovery of key molecules or pathways to interrupt the parasites’ infection and reinfection. This relies not only on the similarities found between G. lamblia and other cell types but also on the differences. The findings described in this review show the importance of the function of membrane-associated proteins. However, there are still many unanswered questions: how do the expression and membrane association of proteins, like FYVE domain- and PX domain-containing proteins, impact on the origin, maintenance, and function of the PVs? Are lipid modifications critical for HCNCp localization, function, or both? Does the differentiation process change protein localization in trophozoite differentiation into the cyst or vice versa? Answers to these and other emergent questions will certainly help in understanding the peculiarities of G. lamblia and in the design of more specific anti-giardial drugs.

Funding

This research was funded in part by Agencia Nacional para la Promoción Científica y Tecnológica (FONCYT) PICT 2013-1122 and PICT 2013-1118.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Adl, S.M.; Simpson, A.G.; Farmer, M.A.; Andersen, R.A.; Anderson, O.R.; Barta, J.R.; Bowser, S.S.; Brugerolle, G.; Fensome, R.A.; Fredericq, S.; et al. The new higher level classification of eukaryotes with emphasis on the taxonomy of protists. J. Eukaryot. Microbiol. 2005, 52, 399–451. [Google Scholar] [CrossRef] [PubMed]
  2. Ankarklev, J.; Jerlstrom-Hultqvist, J.; Ringqvist, E.; Troell, K.; Svard, S.G. Behind the smile: Cell biology and disease mechanisms of Giardia species. Nat. Rev. Microbiol. 2010, 8, 413–422. [Google Scholar] [CrossRef] [PubMed]
  3. Muller, J.; Ley, S.; Felger, I.; Hemphill, A.; Muller, N. Identification of differentially expressed genes in a Giardia lamblia WB C6 clone resistant to nitazoxanide and metronidazole. J. Antimicrob. Chemother. 2008, 62, 72–82. [Google Scholar] [CrossRef] [PubMed]
  4. Soltys, B.J.; Falah, M.; Gupta, R.S. Identification of endoplasmic reticulum in the primitive eukaryote Giardia lamblia using cryoelectron microscopy and antibody to bip. J. Cell Sci. 1996, 109 Pt 7, 1909–1917. [Google Scholar]
  5. Tovar, J.; Leon-Avila, G.; Sanchez, L.B.; Sutak, R.; Tachezy, J.; van der Giezen, M.; Hernandez, M.; Muller, M.; Lucocq, J.M. Mitochondrial remnant organelles of Giardia function in iron-sulphur protein maturation. Nature 2003, 426, 172–176. [Google Scholar] [CrossRef] [PubMed]
  6. Lanfredi-Rangel, A.; Attias, M.; de Carvalho, T.M.; Kattenbach, W.M.; De Souza, W. The peripheral vesicles of trophozoites of the primitive protozoan Giardia lamblia may correspond to early and late endosomes and to lysosomes. J. Struct. Biol. 1998, 123, 225–235. [Google Scholar] [CrossRef] [PubMed]
  7. Touz, M.C.; Zamponi, N. Sorting without a Golgi complex. Traffic 2017, 18, 637–645. [Google Scholar] [CrossRef] [PubMed]
  8. Yichoy, M.; Duarte, T.T.; De Chatterjee, A.; Mendez, T.L.; Aguilera, K.Y.; Roy, D.; Roychowdhury, S.; Aley, S.B.; Das, S. Lipid metabolism in giardia: A post-genomic perspective. Parasitology 2011, 138, 267–278. [Google Scholar] [CrossRef] [PubMed]
  9. Yichoy, M.; Nakayasu, E.S.; Shpak, M.; Aguilar, C.; Aley, S.B.; Almeida, I.C.; Das, S. Lipidomic analysis reveals that phosphatidylglycerol and phosphatidylethanolamine are newly generated phospholipids in an early-divergent protozoan, Giardia lamblia. Mol. Biochem. Parasitol. 2009, 165, 67–78. [Google Scholar] [CrossRef] [PubMed]
  10. Behnia, R.; Munro, S. Organelle identity and the signposts for membrane traffic. Nature 2005, 438, 597–604. [Google Scholar] [CrossRef] [PubMed]
  11. Di Paolo, G.; De Camilli, P. Phosphoinositides in cell regulation and membrane dynamics. Nature 2006, 443, 651–657. [Google Scholar] [CrossRef] [PubMed]
  12. Hernandez, Y.; Zamora, G.; Ray, S.; Chapoy, J.; Chavez, E.; Valvarde, R.; Williams, E.; Aley, S.B.; Das, S. Transcriptional analysis of three major putative phosphatidylinositol kinase genes in a parasitic protozoan, Giardia lamblia. J. Eukaryot. Microbiol. 2007, 54, 29–32. [Google Scholar] [CrossRef] [PubMed]
  13. Cox, S.S.; van der Giezen, M.; Tarr, S.J.; Crompton, M.R.; Tovar, J. Evidence from bioinformatics, expression and inhibition studies of phosphoinositide-3 kinase signalling in Giardia intestinalis. BMC Microbiol. 2006, 6, 45. [Google Scholar] [CrossRef] [PubMed]
  14. Sinha, A.; Mandal, S.; Banerjee, S.; Ghosh, A.; Ganguly, S.; Sil, A.K.; Sarkar, S. Identification and characterization of a fyve domain from the early diverging eukaryote Giardia lamblia. Curr. Microbiol. 2011, 62, 1179–1184. [Google Scholar] [CrossRef] [PubMed]
  15. Gillooly, D.J.; Morrow, I.C.; Lindsay, M.; Gould, R.; Bryant, N.J.; Gaullier, J.M.; Parton, R.G.; Stenmark, H. Localization of phosphatidylinositol 3-phosphate in yeast and mammalian cells. EMBO J. 2000, 19, 4577–4588. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  16. Itoh, T.; Koshiba, S.; Kigawa, T.; Kikuchi, A.; Yokoyama, S.; Takenawa, T. Role of the ENTH domain in phosphatidylinositol-4,5-bisphosphate binding and endocytosis. Science 2001, 291, 1047–1051. [Google Scholar] [CrossRef] [PubMed]
  17. Hirst, J.; Motley, A.; Harasaki, K.; Peak Chew, S.Y.; Robinson, M.S. EpsinR: An ENTH domain-containing protein that interacts with AP-1. Mol. Biol. Cell 2003, 14, 625–641. [Google Scholar] [CrossRef] [PubMed]
  18. Feliziani, C.; Zamponi, N.; Gottig, N.; Ropolo, A.S.; Lanfredi-Rangel, A.; Touz, M.C. The giardial ENTH protein participates in lysosomal protein trafficking and endocytosis. Biochim. Biophys. Acta 2015, 1853, 646–659. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  19. Ebneter, J.A.; Hehl, A.B. The single epsin homolog in Giardia lamblia localizes to the ventral disk of trophozoites and is not associated with clathrin membrane coats. Mol. Biochem. Parasitol. 2014, 197, 24–27. [Google Scholar] [CrossRef] [PubMed]
  20. Odorizzi, G.; Babst, M.; Emr, S.D. Phosphoinositide signaling and the regulation of membrane trafficking in yeast. Trends Biochem. Sci. 2000, 25, 229–235. [Google Scholar] [CrossRef]
  21. Worby, C.A.; Dixon, J.E. Sorting out the cellular functions of sorting nexins. Nat. Rev. Mol. Cell Biol. 2002, 3, 919–931. [Google Scholar] [CrossRef] [PubMed]
  22. Karathanassis, D.; Stahelin, R.V.; Bravo, J.; Perisic, O.; Pacold, C.M.; Cho, W.; Williams, R.L. Binding of the PX domain of p47(phox) to phosphatidylinositol 3,4-bisphosphate and phosphatidic acid is masked by an intramolecular interaction. EMBO J. 2002, 21, 5057–5068. [Google Scholar] [CrossRef] [PubMed]
  23. Xu, J.; Liu, D.; Gill, G.; Songyang, Z. Regulation of cytokine-independent survival kinase (CISK) by the Phox homology domain and phosphoinositides. J. Cell Biol. 2001, 154, 699–705. [Google Scholar] [CrossRef] [PubMed]
  24. Seet, L.F.; Hong, W. The phox (PX) domain proteins and membrane traffic. Biochim. Biophys. Acta 2006, 1761, 878–896. [Google Scholar] [CrossRef] [PubMed]
  25. Jana, A.; Sinha, A.; Sarkar, S. Phosphoinositide binding profiles of the PX domains of Giardia lamblia. Parasitol. Int. 2017, 66, 606–614. [Google Scholar] [CrossRef] [PubMed]
  26. Teasdale, R.D.; Loci, D.; Houghton, F.; Karlsson, L.; Gleeson, P.A. A large family of endosome-localized proteins related to sorting nexin 1. Biochem. J. 2001, 358, 7–16. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  27. Gallon, M.; Cullen, P.J. Retromer and sorting nexins in endosomal sorting. Biochem. Soc. Trans. 2015, 43, 33–47. [Google Scholar] [CrossRef] [PubMed]
  28. Bonifacino, J.S.; Hurley, J.H. Retromer. Curr. Opin. Cell Biol. 2008, 20, 427–436. [Google Scholar] [CrossRef] [PubMed]
  29. Harterink, M.; Port, F.; Lorenowicz, M.J.; McGough, I.J.; Silhankova, M.; Betist, M.C.; van Weering, J.R.; van Heesbeen, R.G.; Middelkoop, T.C.; Basler, K.; et al. A SNX3-dependent retromer pathway mediates retrograde transport of the Wnt sorting receptor Wntless and is required for Wnt secretion. Nat. Cell Biol. 2011, 13, 914–923. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  30. Phillips, S.A.; Barr, V.A.; Haft, D.H.; Taylor, S.I.; Haft, C.R. Identification and characterization of SNX15, a novel sorting nexin involved in protein trafficking. J. Biol. Chem. 2001, 276, 5074–5084. [Google Scholar] [CrossRef] [PubMed]
  31. Van Kerkhof, P.; Lee, J.; McCormick, L.; Tetrault, E.; Lu, W.; Schoenfish, M.; Oorschot, V.; Strous, G.J.; Klumperman, J.; Bu, G. Sorting nexin 17 facilitates LRP recycling in the early endosome. EMBO J. 2005, 24, 2851–2861. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  32. Miras, S.L.; Merino, M.C.; Gottig, N.; Ropolo, A.S.; Touz, M.C. The giardial VPS35 retromer subunit is necessary for multimeric complex assembly and interaction with the vacuolar protein sorting receptor. Biochim. Biophys. Acta 2013, 1833, 2628–2638. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  33. Cheever, M.L.; Sato, T.K.; de Beer, T.; Kutateladze, T.G.; Emr, S.D.; Overduin, M. Phox domain interaction with Ptdins(3)P targets the Vam7 t-SNARE to vacuole membranes. Nat. Cell Biol. 2001, 3, 613–618. [Google Scholar] [CrossRef] [PubMed]
  34. Elias, E.V.; Quiroga, R.; Gottig, N.; Nakanishi, H.; Nash, T.E.; Neiman, A.; Lujan, H.D. Characterization of SNARES determines the absence of a typical Golgi apparatus in the ancient eukaryote Giardia lamblia. J. Biol. Chem. 2008, 283, 35996–36010. [Google Scholar] [CrossRef] [PubMed]
  35. Settembre, C.; Fraldi, A.; Medina, D.L.; Ballabio, A. Signals from the lysosome: A control centre for cellular clearance and energy metabolism. Nat. Rev. Mol. Cell Biol. 2013, 14, 283–296. [Google Scholar] [CrossRef] [PubMed]
  36. Blott, E.J.; Griffiths, G.M. Secretory lysosomes. Nat. Rev. Mol. Cell Biol. 2002, 3, 122–131. [Google Scholar] [CrossRef] [PubMed]
  37. Rivero, M.R.; Jausoro, I.; Bisbal, M.; Feliziani, C.; Lanfredi-Rangel, A.; Touz, M.C. Receptor-mediated endocytosis and trafficking between endosomal-lysosomal vacuoles in Giardia lamblia. Parasitol. Res. 2013, 112, 1813–1818. [Google Scholar] [CrossRef] [PubMed]
  38. Rivero, M.R.; Vranych, C.V.; Bisbal, M.; Maletto, B.A.; Ropolo, A.S.; Touz, M.C. Adaptor protein 2 regulates receptor-mediated endocytosis and cyst formation in Giardia lamblia. Biochem. J. 2010, 428, 33–45. [Google Scholar] [CrossRef] [PubMed]
  39. Bonifacino, J.S.; Lippincott-Schwartz, J. Coat proteins: Shaping membrane transport. Nat. Rev. Mol. Cell Biol. 2003, 4, 409–414. [Google Scholar] [CrossRef] [PubMed]
  40. Janvier, K.; Kato, Y.; Boehm, M.; Rose, J.R.; Martina, J.A.; Kim, B.Y.; Venkatesan, S.; Bonifacino, J.S. Recognition of dileucine-based sorting signals from HIV-1 NEF and LIMP-II by the AP-1 γ-σ1 and AP-3 δ- σ3 hemicomplexes. J. Cell Biol. 2003, 163, 1281–1290. [Google Scholar] [CrossRef] [PubMed]
  41. Doray, B.; Lee, I.; Knisely, J.; Bu, G.; Kornfeld, S. The γ/σ1 and α/σ2 hemicomplexes of clathrin adaptors AP-1 and AP-2 harbor the dileucine recognition site. Mol. Biol. Cell 2007, 18, 1887–1896. [Google Scholar] [CrossRef] [PubMed]
  42. Touz, M.C.; Lujan, H.D.; Hayes, S.F.; Nash, T.E. Sorting of encystation-specific cysteine protease to lysosome-like peripheral vacuoles in Giardia lamblia requires a conserved tyrosine-based motif. J. Biol. Chem. 2003, 278, 6420–6426. [Google Scholar] [CrossRef] [PubMed]
  43. Touz, M.C.; Kulakova, L.; Nash, T.E. Adaptor protein complex 1 mediates the transport of lysosomal proteins from a Golgi-like organelle to peripheral vacuoles in the primitive eukaryote Giardia lamblia. Mol. Biol. Cell 2004, 15, 3053–3060. [Google Scholar] [CrossRef] [PubMed]
  44. Ghosh, P.; Dahms, N.M.; Kornfeld, S. Mannose 6-phosphate receptors: New twists in the tale. Nat. Rev. Mol. Cell Biol. 2003, 4, 202–212. [Google Scholar] [CrossRef] [PubMed]
  45. Rivero, M.R.; Miras, S.L.; Feliziani, C.; Zamponi, N.; Quiroga, R.; Hayes, S.F.; Ropolo, A.S.; Touz, M.C. Vacuolar protein sorting receptor in Giardia lamblia. PLoS ONE 2012, 7, e43712. [Google Scholar] [CrossRef] [PubMed]
  46. Rivero, M.R.; Miras, S.L.; Quiroga, R.; Ropolo, A.S.; Touz, M.C. Giardia lamblia low-density lipoprotein receptor-related protein is involved in selective lipoprotein endocytosis and parasite replication. Mol. Microbiol. 2011, 79, 1204–1219. [Google Scholar] [CrossRef] [PubMed]
  47. Martincova, E.; Voleman, L.; Pyrih, J.; Zarsky, V.; Vondrackova, P.; Kolisko, M.; Tachezy, J.; Dolezal, P. Probing the biology of Giardia intestinalis mitosomes using in vivo enzymatic tagging. Mol. Cell. Biol. 2015, 35, 2864–2874. [Google Scholar] [CrossRef] [PubMed]
  48. Touz, M.C.; Conrad, J.T.; Nash, T.E. A novel palmitoyl acyl transferase controls surface protein palmitoylation and cytotoxicity in Giardia lamblia. Mol. Microbiol. 2005, 58, 999–1011. [Google Scholar] [CrossRef] [PubMed]
  49. Li, W.; Saraiya, A.A.; Wang, C.C. Experimental verification of the identity of variant-specific surface proteins in Giardia lamblia trophozoites. mBio 2013, 4, e00321-13. [Google Scholar] [CrossRef] [PubMed]
  50. Mowatt, M.R.; Aggarwal, A.; Nash, T.E. Carboxy-terminal sequence conservation among variant-specific surface proteins of Giardia lamblia. Mol. Biochem. Parasitol. 1991, 49, 215–227. [Google Scholar] [CrossRef]
  51. Adam, R.D. Biology of Giardia lamblia. Clin. Microbiol. Rev. 2001, 14, 447–475. [Google Scholar] [CrossRef] [PubMed]
  52. Nash, T.E.; Mowatt, M.R. Characterization of a Giardia lamblia variant-specific surface protein (VSP) gene from isolate GS/M and estimation of the VSP gene repertoire size. Mol. Biochem. Parasitol. 1992, 51, 219–227. [Google Scholar] [CrossRef]
  53. Aley, S.B.; Gillin, F.D. Specialized surface adaptations of Giardia lamblia. Infect. Agents Dis. 1995, 4, 161–166. [Google Scholar] [PubMed]
  54. Svard, S.G.; Meng, T.C.; Hetsko, M.L.; McCaffery, J.M.; Gillin, F.D. Differentiation-associated surface antigen variation in the ancient eukaryote Giardia lamblia. Mol. Microbiol. 1998, 30, 979–989. [Google Scholar] [CrossRef] [PubMed]
  55. Nash, T.E.; Banks, S.M.; Alling, D.W.; Merritt, J.W., Jr.; Conrad, J.T. Frequency of variant antigens in Giardia lamblia. Exp. Parasitol. 1990, 71, 415–421. [Google Scholar] [CrossRef]
  56. Nash, T.E.; Herrington, D.A.; Levine, M.M.; Conrad, J.T.; Merritt, J.W., Jr. Antigenic variation of Giardia lamblia in experimental human infections. J. Immunol. 1990, 144, 4362–4369. [Google Scholar] [PubMed]
  57. Nash, T. Surface antigen variability and variation in Giardia lamblia. Parasitol. Today 1992, 8, 229–234. [Google Scholar] [CrossRef]
  58. Gottstein, B.; Harriman, G.R.; Conrad, J.T.; Nash, T.E. Antigenic variation in Giardia lamblia: Cellular and humoral immune response in a mouse model. Parasite Immunol. 1990, 12, 659–673. [Google Scholar] [CrossRef] [PubMed]
  59. Papanastasiou, P.; Hiltpold, A.; Bommeli, C.; Kohler, P. The release of the variant surface protein of Giardia to its soluble isoform is mediated by the selective cleavage of the conserved carboxy-terminal domain. Biochemistry 1996, 35, 10143–10148. [Google Scholar] [CrossRef] [PubMed]
  60. Marti, M.; Li, Y.; Schraner, E.M.; Wild, P.; Kohler, P.; Hehl, A.B. The secretory apparatus of an ancient eukaryote: Protein sorting to separate export pathways occurs before formation of transient Golgi-like compartments. Mol. Biol. Cell 2003, 14, 1433–1447. [Google Scholar] [CrossRef] [PubMed]
  61. Touz, M.C.; Ropolo, A.S.; Rivero, M.R.; Vranych, C.V.; Conrad, J.T.; Svard, S.G.; Nash, T.E. Arginine deiminase has multiple regulatory roles in the biology of Giardia lamblia. J. Cell Sci. 2008, 121, 2930–2938. [Google Scholar] [CrossRef] [PubMed]
  62. Papanastasiou, P.; McConville, M.J.; Ralton, J.; Kohler, P. The variant-specific surface protein of Giardia, VSP4A1, is a glycosylated and palmitoylated protein. Biochem. J. 1997, 322 Pt 1, 49–56. [Google Scholar] [CrossRef]
  63. Hiltpold, A.; Frey, M.; Hulsmeier, A.; Kohler, P. Glycosylation and palmitoylation are common modifications of Giardia variant surface proteins. Mol. Biochem. Parasitol. 2000, 109, 61–65. [Google Scholar] [CrossRef]
  64. Carranza, P.G.; Feltes, G.; Ropolo, A.; Quintana, S.M.; Touz, M.C.; Lujan, H.D. Simultaneous expression of different variant-specific surface proteins in single Giardia lamblia trophozoites during encystation. Infect. Immun. 2002, 70, 5265–5268. [Google Scholar] [CrossRef] [PubMed]
  65. Davids, B.J.; Reiner, D.S.; Birkeland, S.R.; Preheim, S.P.; Cipriano, M.J.; McArthur, A.G.; Gillin, F.D. A new family of giardial cysteine-rich non-VSP protein genes and a novel cyst protein. PLoS ONE 2006, 1, e44. [Google Scholar] [CrossRef] [PubMed]
  66. Merino, M.C.; Zamponi, N.; Vranych, C.V.; Touz, M.C.; Ropolo, A.S. Identification of Giardia lamblia DHHC proteins and the role of protein S-palmitoylation in the encystation process. PLoS Negl. Trop. Dis. 2014, 8, e2997. [Google Scholar] [CrossRef] [PubMed]
  67. Bartels, D.J.; Mitchell, D.A.; Dong, X.; Deschenes, R.J. Erf2, a novel gene product that affects the localization and palmitoylation of Ras2 in Saccharomyces cerevisiae. Mol. Cell. Biol. 1999, 19, 6775–6787. [Google Scholar] [CrossRef] [PubMed]
  68. Lobo, S.; Greentree, W.K.; Linder, M.E.; Deschenes, R.J. Identification of a Ras palmitoyltransferase in Saccharomyces cerevisiae. J. Biol. Chem. 2002, 277, 41268–41273. [Google Scholar] [CrossRef] [PubMed]
  69. Linder, M.E.; Jennings, B.C. Mechanism and function of DHHC S-acyltransferases. Biochem. Soc. Trans. 2013, 41, 29–34. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  70. Coelho, C.H.; Costa, A.O.; Silva, A.C.; Pucci, M.M.; Serufo, A.V.; Busatti, H.G.; Durigan, M.; Perales, J.; Chapeaurouge, A.; de Silva a Silva, D.A.; et al. Genotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia duodenalis, infective to mice. PLoS ONE 2016, 11, e0164946. [Google Scholar] [CrossRef] [PubMed]
  71. Emery, S.J.; Lacey, E.; Haynes, P.A. Quantitative proteomics in Giardia duodenalis-achievements and challenges. Mol. Biochem. Parasitol. 2016, 208, 96–112. [Google Scholar] [CrossRef] [PubMed]
  72. Wampfler, P.B.; Tosevski, V.; Nanni, P.; Spycher, C.; Hehl, A.B. Proteomics of secretory and endocytic organelles in Giardia lamblia. PLoS ONE 2014, 9, e94089. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  73. Jerlstrom-Hultqvist, J.; Ankarklev, J.; Svard, S.G. Is human giardiasis caused by two different Giardia species? Gut Microbes 2011, 1, 379–382. [Google Scholar] [CrossRef] [PubMed]
  74. Franzen, O.; Jerlstrom-Hultqvist, J.; Castro, E.; Sherwood, E.; Ankarklev, J.; Reiner, D.S.; Palm, D.; Andersson, J.O.; Andersson, B.; Svard, S.G. Draft genome sequencing of Giardia intestinalis assemblage B isolate GS: Is human giardiasis caused by two different species? PLoS Pathog. 2009, 5, e1000560. [Google Scholar] [CrossRef] [PubMed]
  75. Singer, S.M.; Yee, J.; Nash, T.E. Episomal and integrated maintenance of foreign DNA in Giardia lamblia. Mol. Biochem. Parasitol. 1998, 92, 59–69. [Google Scholar] [CrossRef]
  76. Kulakova, L.; Singer, S.M.; Conrad, J.; Nash, T.E. Epigenetic mechanisms are involved in the control of Giardia lamblia antigenic variation. Mol. Microbiol. 2006, 61, 1533–1542. [Google Scholar] [CrossRef] [PubMed]
Genes 09 00404 g001 550
Figure 1. Variant-specific surface protein or VSP. (A) Confocal immunofluorescence image showing the plasma membrane localization of the VSP9B10 (green) by using FITC-labeled anti-9B10 mAb. The nuclei are stained with DAPI (blue). Scale bars: 5 μm. VSP9B10 covers two of the trophozoites observed. (B) Schematic representation of a VSP containing a signal peptide, an extracellular variable domain, a transmembrane (TM) domain, and the CRGKA invariable cytoplasmic tail. The sites of palmitoylation (underlined C) and citrullination (the asterisk in R) of the cytoplasmic tail are denoted.
Figure 1. Variant-specific surface protein or VSP. (A) Confocal immunofluorescence image showing the plasma membrane localization of the VSP9B10 (green) by using FITC-labeled anti-9B10 mAb. The nuclei are stained with DAPI (blue). Scale bars: 5 μm. VSP9B10 covers two of the trophozoites observed. (B) Schematic representation of a VSP containing a signal peptide, an extracellular variable domain, a transmembrane (TM) domain, and the CRGKA invariable cytoplasmic tail. The sites of palmitoylation (underlined C) and citrullination (the asterisk in R) of the cytoplasmic tail are denoted.
Genes 09 00404 g001
Genes 09 00404 g002 550
Figure 2. Schematic representation of Giardia lamblia proteins localization. Left panel, confocal microscopy merged image showing differential interference contrast, encystation-specific cysteine protease (ESCP)-HA localization in the PVs (green), and the nuclei labelled with DAPI (blue). The right panel shows a draw of proteins that travel from the plasma membrane to the PVs (green arrow), those that use the retromer transport (red arrow), the ones that go from the ER to the PVs (blue arrow), and the integral membrane proteins. Only the proteins with demonstrated experimental localization are denoted. SNXs: sorting nexins.
Figure 2. Schematic representation of Giardia lamblia proteins localization. Left panel, confocal microscopy merged image showing differential interference contrast, encystation-specific cysteine protease (ESCP)-HA localization in the PVs (green), and the nuclei labelled with DAPI (blue). The right panel shows a draw of proteins that travel from the plasma membrane to the PVs (green arrow), those that use the retromer transport (red arrow), the ones that go from the ER to the PVs (blue arrow), and the integral membrane proteins. Only the proteins with demonstrated experimental localization are denoted. SNXs: sorting nexins.
Genes 09 00404 g002
Table
Table 1. The phagocyte oxidase (PX) domain-containing proteins in Giardia lamblia, Assemble A: phosphoinositide-binding specificities, localization and expression. PIP: phosphoinositide; PVs: peripheral vacuoles; ND: not determined; mRNA: messenger RNA.
Table 1. The phagocyte oxidase (PX) domain-containing proteins in Giardia lamblia, Assemble A: phosphoinositide-binding specificities, localization and expression. PIP: phosphoinositide; PVs: peripheral vacuoles; ND: not determined; mRNA: messenger RNA.
GL50803_16596GL50803_42357GL50803_16548GL50803_24488GL50803_16595GL50803_7723
Basic amino acids--++++
PX-domain PIP binding (ordered by affinity)PI3PPI3P
PI5P
PI3,4,5P3
PI3,5P2
PI4,5P2
PI4P		PI3,4P2
PI3,5P2
PI4,5P2
PI3P
PI4P
PI3P		PI3,4,5P3
PI3,4P2
PI3,5P2
PI4,5P2
PI3P
PI4P
PI3P		PI3,5P2
PI3P
PI4P
PI3P
PI4,5P2		ND
Localization in yeastEndosomesEndosomesNDNDCytosolic vesicles Golgi?Vacuole membrane
Localization in G. lambliaNDNDPVsNDNDND
mRNA expression++ (mayor expression in cysts)++ (mayor expression in cysts)++
Table
Table 2. Orthologs of the membrane-associated proteins of Giardia described in the EuPathDB GDB (http://giardiadb.org/giardiadb/). The Gene ID numbers of the proteins described in this work are denoted in light blue. NO: no orthologs. The VSPs are assemblage-specific (*).
Table 2. Orthologs of the membrane-associated proteins of Giardia described in the EuPathDB GDB (http://giardiadb.org/giardiadb/). The Gene ID numbers of the proteins described in this work are denoted in light blue. NO: no orthologs. The VSPs are assemblage-specific (*).
Giardia Ortholog Groups
assemblage A assemblage A2assemblage B assemblage E
MotivIsolate WBIsolate DHIsolate GS_BIsolate GSIsolate P15
FYVEGL50803_16653DHA2_16653GSB_16653 GL50581_4212GLP15_3543
ENTHGL50803_3256DHA2_151051GSB_151711GL50581_49GLP15_2242
PXGL50803_16596DHA2_150243GSB_150230NOGLP15_442
GL50803_42357DHA2_150236GSB_150235GL50581_3587GLP15_433
GL50803_16548DHA2_16548GSB_16548GL50581_1134GLP15_3433
GL50803_24488DHA2_152720GSB_154119GL50581_3300GLP15_1942
GL50803_7723DHA2_150244GSB_150229GL50581_444GLP15_443
GL50803_16595DHA2_150245GSB_150228GL50581_445GLP15_444
YXXØGL50803_14566DHA2_14566GSB_14566GL50581_1260GLP15_3781
GL50803_28954DHA2_28954GSB_28954GL50581_1526GLP15_2327
FXNPXYGL50803_113565DHA2_152495GSB_153075NOGLP15_1378
GL50803_94510DHA2_153693 GLP15_1686
CRGKA (VSP)--GSB_150963 *--
GL50803_101074 *----
GL50803_112208 *----
CRRSKAV (HCNCp)GL50803_40376DHA2_40376GSB_40376GL50581_1041GLP15_486
DHHCGL50803_42184DHA2_153342GSB_154139GL50581_1824GLP15_189
GL50803_8711DHA2_150040GSB_150746GL50581_3650GLP15_196

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Touz, M.C.; Feliziani, C.; Rópolo, A.S. Membrane-Associated Proteins in Giardia lamblia. Genes 2018, 9, 404. https://doi.org/10.3390/genes9080404

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Touz MC, Feliziani C, Rópolo AS. Membrane-Associated Proteins in Giardia lamblia. Genes. 2018; 9(8):404. https://doi.org/10.3390/genes9080404

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Touz, María C., Constanza Feliziani, and Andrea S. Rópolo. 2018. "Membrane-Associated Proteins in Giardia lamblia" Genes 9, no. 8: 404. https://doi.org/10.3390/genes9080404

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Touz, M. C., Feliziani, C., & Rópolo, A. S. (2018). Membrane-Associated Proteins in Giardia lamblia. Genes, 9(8), 404. https://doi.org/10.3390/genes9080404

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