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RAD4 and RAD23/HMR Contribute to Arabidopsis UV Tolerance

Department of Biological Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
Author to whom correspondence should be addressed.
Received: 12 October 2017 / Revised: 15 December 2017 / Accepted: 19 December 2017 / Published: 28 December 2017
(This article belongs to the Special Issue DNA Damage Responses in Plants)
In plants, exposure to solar ultraviolet (UV) light is unavoidable, resulting in DNA damage. Damaged DNA causes mutations, replication arrest, and cell death, thus efficient repair of the damaged DNA is essential. A light-independent DNA repair pathway called nucleotide excision repair (NER) is conserved throughout evolution. For example, the damaged DNA-binding protein Radiation sensitive 4 (Rad4) in Saccharomyces cerevisiae is homologous to the mammalian NER protein Xeroderma Pigmentosum complementation group C (XPC). In this study, we examined the role of the Arabidopsis thaliana Rad4/XPC homologue (AtRAD4) in plant UV tolerance by generating overexpression lines. AtRAD4 overexpression, both with and without an N-terminal yellow fluorescent protein (YFP) tag, resulted in increased UV tolerance. YFP-RAD4 localized to the nucleus, and UV treatment did not alter this localization. We also used yeast two-hybrid analysis to examine the interaction of AtRAD4 with Arabidopsis RAD23 and found that RAD4 interacted with RAD23B as well as with the structurally similar protein HEMERA (HMR). In addition, we found that hmr and rad23 mutants exhibited increased UV sensitivity. Thus, our analysis suggests a role for RAD4 and RAD23/HMR in plant UV tolerance. View Full-Text
Keywords: XPC/RAD4; RAD23; HEMERA; Arabidopsis; UV tolerance; nucleotide excision repair XPC/RAD4; RAD23; HEMERA; Arabidopsis; UV tolerance; nucleotide excision repair
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MDPI and ACS Style

Lahari, T.; Lazaro, J.; Schroeder, D.F. RAD4 and RAD23/HMR Contribute to Arabidopsis UV Tolerance. Genes 2018, 9, 8.

AMA Style

Lahari T, Lazaro J, Schroeder DF. RAD4 and RAD23/HMR Contribute to Arabidopsis UV Tolerance. Genes. 2018; 9(1):8.

Chicago/Turabian Style

Lahari, Triparna, Janelle Lazaro, and Dana F. Schroeder. 2018. "RAD4 and RAD23/HMR Contribute to Arabidopsis UV Tolerance" Genes 9, no. 1: 8.

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