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Sibling sRNA RyfA1 Influences Shigella dysenteriae Pathogenesis

Department of Biological Sciences, Ohio University, 1 Ohio University Drive Athens, Athens, OH 45701, USA
OU Genomics Core Facility, Ohio University, 1 Ohio University Drive Athens, Athens, OH 45701, USA
Department of Biomedical Sciences, Ohio University Heritage College of Osteopathic Medicine, 1 Ohio University Drive Athens, Athens, OH 45701, USA
Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, VA-MD College of Veterinary Medicine, Virginia Tech, 1410 Prices Fork Rd., Blacksburg, VA 24060, USA
Author to whom correspondence should be addressed.
Genes 2017, 8(2), 50;
Received: 9 October 2016 / Accepted: 17 January 2017 / Published: 26 January 2017
(This article belongs to the Special Issue Virulence Gene Regulation in Bacteria)
PDF [2529 KB, uploaded 2 February 2017]


Small regulatory RNAs (sRNAs) of Shigella dysenteriae and other pathogens are vital for the regulation of virulence-associated genes and processes. Here, we characterize RyfA1, one member of a sibling pair of sRNAs produced by S. dysenteriae. Unlike its nearly identical sibling molecule, RyfA2, predicted to be encoded almost exclusively by non-pathogenic species, the presence of a gene encoding RyfA1, or a RyfA1-like molecule, is strongly correlated with virulence in a variety of enteropathogens. In S. dysenteriae, the overproduction of RyfA1 negatively impacts the virulence-associated process of cell-to-cell spread as well as the expression of ompC, a gene encoding a major outer membrane protein important for the pathogenesis of Shigella. Interestingly, the production of RyfA1 is controlled by a second sRNA, here termed RyfB1, the first incidence of one regulatory small RNA controlling another in S. dysenteriae or any Shigella species. View Full-Text
Keywords: Shigella; sRNA; pathogenesis; riboregulators; sibling; OmpC Shigella; sRNA; pathogenesis; riboregulators; sibling; OmpC

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Fris, M.E.; Broach, W.H.; Klim, S.E.; Coschigano, P.W.; Carroll, R.K.; Caswell, C.C.; Murphy, E.R. Sibling sRNA RyfA1 Influences Shigella dysenteriae Pathogenesis. Genes 2017, 8, 50.

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