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Genetic Analysis of the Major Capsid Protein of the Archaeal Fusellovirus SSV1: Mutational Flexibility and Conformational Change

Center for Life in Extreme Environments, Biology Department, Portland State University, P.O. Box 751, Portland, OR 97207-0751, USA
Author to whom correspondence should be addressed.
Genes 2017, 8(12), 373;
Received: 14 November 2017 / Revised: 29 November 2017 / Accepted: 1 December 2017 / Published: 8 December 2017
(This article belongs to the Special Issue Genetics and Genomics of Extremophiles)
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Viruses with spindle or lemon-shaped virions are rare in the world of viruses, but are common in viruses of archaeal extremophiles, possibly due to the extreme conditions in which they thrive. However, the structural and genetic basis for the unique spindle shape is unknown. The best-studied spindle-shaped virus, Sulfolobus Spindle-shaped Virus 1 (SSV1), is composed mostly of the major capsid protein VP1. Similar to many other viruses, proteolytic cleavage of VP1 is thought to be critical for virion formation. Unlike half of the genes in SSV1, including the minor capsid protein gene VP3, the VP1 gene does not tolerate deletion or transposon insertion. To determine the role of the VP1 gene and its proteolysis for virus function, we developed techniques for site-directed mutagenesis of the SSV1 genome and complemented deletion mutants with VP1 genes from other SSVs. By analyzing these mutants, we demonstrate that the N-terminus of the VP1 protein is required, but the N-terminus, or entire SSV1 VP1 protein, can be exchanged with VP1s from other SSVs. However, the conserved glutamate at the cleavage site is not essential for infectivity. Interestingly, viruses containing point mutations at this position generate mostly abnormal virions. View Full-Text
Keywords: proteolysis; site-directed mutagenesis; complementation; mutants; morphology proteolysis; site-directed mutagenesis; complementation; mutants; morphology

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Iverson, E.A.; Goodman, D.A.; Gorchels, M.E.; Stedman, K.M. Genetic Analysis of the Major Capsid Protein of the Archaeal Fusellovirus SSV1: Mutational Flexibility and Conformational Change. Genes 2017, 8, 373.

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