Spatiotemporal Expression Characterization of KRTAP6 Family Genes and Its Effect on Wool Traits

Sun, Hongxian; He, Zhaohua; Zhao, Fangfang; Hu, Jiang; Wang, Jiqing; Liu, Xiu; Zhao, Zhidong; Li, Mingna; Luo, Yuzhu; Li, Shaobin

doi:10.3390/genes15010095

Open AccessArticle

Spatiotemporal Expression Characterization of KRTAP6 Family Genes and Its Effect on Wool Traits

by

Hongxian Sun

,

Zhaohua He

,

Fangfang Zhao

,

Jiang Hu

,

Jiqing Wang

,

Xiu Liu

,

Zhidong Zhao

,

Mingna Li

,

Yuzhu Luo

and

Shaobin Li

^*

Gansu Key Laboratory of Herbivorous Animal Biotechnology, International Wool Research Institute, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

^*

Author to whom correspondence should be addressed.

Genes 2024, 15(1), 95; https://doi.org/10.3390/genes15010095

Submission received: 18 December 2023 / Revised: 9 January 2024 / Accepted: 10 January 2024 / Published: 14 January 2024

(This article belongs to the Special Issue Genetics and Breeding in Sheep and Goats)

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Abstract

Keratin-related proteins (KAPs) are structural components of wool fibers and are thought to play a key role in regulating the physical and mechanical properties of fibers. Among all KAP genes (KRTAPs), KRTAP6 gene family (KRTAP6-1, KRTAP6-2, KRTAP6-3, KRTAP6-4, and KRTAP6-5) is a very important member with high polymorphism and notable association with some wool traits. In this study, we used real-time fluorescence quantitative PCR (RT-qPCR) and in situ hybridization to investigate spatiotemporal expression of KRTAP6s. The results revealed that KRTAP6 family genes were significantly expressed during anagen compared to other stages (p < 0.05). And it was found the five genes were expressed predominantly in the dermal papillae, inner and outer root sheaths, and showed a distinct spatiotemporal expression pattern. Also, it was found that KRTAP6-1 and KRTAP6-5 mRNA expression was negatively correlated with wool mean fiber diameter (MFD) and mean staple strength (MSS) (p < 0.05). In summary, the KRTAP6 family genes share a similar spatiotemporal expression pattern. And KRTAP6-1 and KRTAP6-5 may regulate the MFD and MSS of Gansu Alpine fine-wool sheep wool by changing the expression.

Keywords: hair follicle; wool traits; spatiotemporal expression; KRTAP6s; sheep

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MDPI and ACS Style

Sun, H.; He, Z.; Zhao, F.; Hu, J.; Wang, J.; Liu, X.; Zhao, Z.; Li, M.; Luo, Y.; Li, S. Spatiotemporal Expression Characterization of KRTAP6 Family Genes and Its Effect on Wool Traits. Genes 2024, 15, 95. https://doi.org/10.3390/genes15010095

AMA Style

Sun H, He Z, Zhao F, Hu J, Wang J, Liu X, Zhao Z, Li M, Luo Y, Li S. Spatiotemporal Expression Characterization of KRTAP6 Family Genes and Its Effect on Wool Traits. Genes. 2024; 15(1):95. https://doi.org/10.3390/genes15010095

Chicago/Turabian Style

Sun, Hongxian, Zhaohua He, Fangfang Zhao, Jiang Hu, Jiqing Wang, Xiu Liu, Zhidong Zhao, Mingna Li, Yuzhu Luo, and Shaobin Li. 2024. "Spatiotemporal Expression Characterization of KRTAP6 Family Genes and Its Effect on Wool Traits" Genes 15, no. 1: 95. https://doi.org/10.3390/genes15010095

APA Style

Sun, H., He, Z., Zhao, F., Hu, J., Wang, J., Liu, X., Zhao, Z., Li, M., Luo, Y., & Li, S. (2024). Spatiotemporal Expression Characterization of KRTAP6 Family Genes and Its Effect on Wool Traits. Genes, 15(1), 95. https://doi.org/10.3390/genes15010095

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Spatiotemporal Expression Characterization of KRTAP6 Family Genes and Its Effect on Wool Traits

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