A Primer for Single-Cell Sequencing in Non-Model Organisms

Alfieri, James M.; Wang, Guosong; Jonika, Michelle M.; Gill, Clare A.; Blackmon, Heath; Athrey, Giridhar N.

doi:10.3390/genes13020380

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A Primer for Single-Cell Sequencing in Non-Model Organisms

by

James M. Alfieri

^1,2,3,*,†,

Guosong Wang

^4,†,

Michelle M. Jonika

^1,5

,

Clare A. Gill

^4,5,

Heath Blackmon

^1,2,5 and

Giridhar N. Athrey

^2,3

¹

Department of Biology, Texas A&M University, College Station, TX 77843, USA

²

Interdisciplinary Program in Ecology and Evolutionary Biology, Texas A&M University, College Station, TX 77843, USA

³

Department of Poultry Science, Texas A&M University, College Station, TX 77843, USA

⁴

Department of Animal Science, Texas A&M University, College Station, TX 77843, USA

⁵

Interdisciplinary Program in Genetics and Genomics, Texas A&M University, College Station, TX 77843, USA

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Genes 2022, 13(2), 380; https://doi.org/10.3390/genes13020380

Submission received: 16 January 2022 / Revised: 12 February 2022 / Accepted: 17 February 2022 / Published: 19 February 2022

(This article belongs to the Special Issue Single-Cell Bioinformatics and Machine Learning)

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Abstract

Single-cell sequencing technologies have led to a revolution in our knowledge of the diversity of cell types, connections between biological levels of organization, and relationships between genotype and phenotype. These advances have mainly come from using model organisms; however, using single-cell sequencing in non-model organisms could enable investigations of questions inaccessible with typical model organisms. This primer describes a general workflow for single-cell sequencing studies and considerations for using non-model organisms (limited to multicellular animals). Importantly, single-cell sequencing, when further applied in non-model organisms, will allow for a deeper understanding of the mechanisms between genotype and phenotype and the basis for biological variation.

Keywords: single-cell sequencing; non-model organism; ecology; evolution; animal science

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MDPI and ACS Style

Alfieri, J.M.; Wang, G.; Jonika, M.M.; Gill, C.A.; Blackmon, H.; Athrey, G.N. A Primer for Single-Cell Sequencing in Non-Model Organisms. Genes 2022, 13, 380. https://doi.org/10.3390/genes13020380

AMA Style

Alfieri JM, Wang G, Jonika MM, Gill CA, Blackmon H, Athrey GN. A Primer for Single-Cell Sequencing in Non-Model Organisms. Genes. 2022; 13(2):380. https://doi.org/10.3390/genes13020380

Chicago/Turabian Style

Alfieri, James M., Guosong Wang, Michelle M. Jonika, Clare A. Gill, Heath Blackmon, and Giridhar N. Athrey. 2022. "A Primer for Single-Cell Sequencing in Non-Model Organisms" Genes 13, no. 2: 380. https://doi.org/10.3390/genes13020380

APA Style

Alfieri, J. M., Wang, G., Jonika, M. M., Gill, C. A., Blackmon, H., & Athrey, G. N. (2022). A Primer for Single-Cell Sequencing in Non-Model Organisms. Genes, 13(2), 380. https://doi.org/10.3390/genes13020380

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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A Primer for Single-Cell Sequencing in Non-Model Organisms

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