Insight into Glyproline Peptides’ Activity through the Modulation of the Inflammatory and Neurosignaling Genetic Response Following Cerebral Ischemia–Reperfusion
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Rat Transient Middle Cerebral Artery Occlusion Model
2.2.1. Operation
2.2.2. Peptide Administration
2.3. Sample Collection and RNA Isolation
2.4. cDNA Synthesis and Real-Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)
2.5. Data Analysis of Real-Time RT-PCR and Statistics
2.6. Functional Analysis
3. Results
3.1. Magnetic Resonance Imaging (MRI)
3.2. Selection of Genes for Studying PGP-Containing Peptides’ Action under Ischemia–Reperfusion (IR) Conditions Based on Our Previous Results
3.3. Analysis of the Effects of PGP and PGPL on the Expression of the IC and NC Genes versus IR’s Effect 24 h after tMCAO
3.4. A Search for DEGs Associated with Semax Treatment Compared to PGP and PGPL Treatment under tMCAO Conditions
3.5. Update of Signaling Pathways Associated with Semax Treatment Based on the Combination of RNA-Seq and PCR Results under the tMCAO Conditions
3.6. The Search for Signaling Pathways That Reflect Common and Unique Gene Expression Effects of PGP, PGPL, and Semax Peptides in IR Conditions
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Stavchansky, V.V.; Filippenkov, I.B.; Remizova, J.A.; Denisova, A.E.; Mozgovoy, I.V.; Gubsky, L.V.; Myasoedov, N.F.; Andreeva, L.A.; Limborska, S.A.; Dergunova, L.V. Insight into Glyproline Peptides’ Activity through the Modulation of the Inflammatory and Neurosignaling Genetic Response Following Cerebral Ischemia–Reperfusion. Genes 2022, 13, 2380. https://doi.org/10.3390/genes13122380
Stavchansky VV, Filippenkov IB, Remizova JA, Denisova AE, Mozgovoy IV, Gubsky LV, Myasoedov NF, Andreeva LA, Limborska SA, Dergunova LV. Insight into Glyproline Peptides’ Activity through the Modulation of the Inflammatory and Neurosignaling Genetic Response Following Cerebral Ischemia–Reperfusion. Genes. 2022; 13(12):2380. https://doi.org/10.3390/genes13122380
Chicago/Turabian StyleStavchansky, Vasily V., Ivan B. Filippenkov, Julia A. Remizova, Alina E. Denisova, Ivan V. Mozgovoy, Leonid V. Gubsky, Nikolay F. Myasoedov, Lyudmila A. Andreeva, Svetlana A. Limborska, and Lyudmila V. Dergunova. 2022. "Insight into Glyproline Peptides’ Activity through the Modulation of the Inflammatory and Neurosignaling Genetic Response Following Cerebral Ischemia–Reperfusion" Genes 13, no. 12: 2380. https://doi.org/10.3390/genes13122380