Next Article in Journal
Clinical and Genetic Characteristics of Korean Congenital Stationary Night Blindness Patients
Next Article in Special Issue
Whole-Body Regeneration in the Lobate Ctenophore Mnemiopsis leidyi
Previous Article in Journal
Network Protein Interaction in the Link between Stroke and Periodontitis Interplay: A Pilot Bioinformatic Analysis
Previous Article in Special Issue
Regeneration Potential of Jellyfish: Cellular Mechanisms and Molecular Insights
Article

Structural and Functional Characterization of the FGF Signaling Pathway in Regeneration of the Polychaete Worm Alitta virens (Annelida, Errantia)

Department of Embryology, St. Petersburg State University, Universitetskaya nab. 7–9, 199034 St. Petersburg, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Uri Gat
Genes 2021, 12(6), 788; https://doi.org/10.3390/genes12060788
Received: 31 December 2020 / Revised: 13 May 2021 / Accepted: 14 May 2021 / Published: 21 May 2021
Epimorphic regeneration of lost body segments is a widespread phenomenon across annelids. However, the molecular inducers of the cell sources for this reparative morphogenesis have not been identified. In this study, we focused on the role of fibroblast growth factor (FGF) signaling in the posterior regeneration of Alitta virens. For the first time, we showed an early activation of FGF ligands and receptor expression in an annelid regenerating after amputation. The expression patterns indicate that the entire regenerative bud is competent to FGFs, whose activity precedes the initiation of cell proliferation. The critical requirement of FGF signaling, especially at early stages, is also supported by inhibitor treatments followed by proliferation assay, demonstrating that induction of blastemal cells depends on FGFs. Our results show that FGF signaling pathway is a key player in regenerative response, while the FGF-positive wound epithelium, ventral nerve cord and some mesodermal cells around the gut could be the inducing tissues. This mechanism resembles reparative regeneration of vertebrate appendages suggesting such a response to the injury may be ancestral for all bilaterians. View Full-Text
Keywords: invertebrates; annelids; Nereis; dedifferentiation; blastema induction; evolution; segmentation; axis elongation; SU5402; fibroblast growth factor invertebrates; annelids; Nereis; dedifferentiation; blastema induction; evolution; segmentation; axis elongation; SU5402; fibroblast growth factor
Show Figures

Figure 1

MDPI and ACS Style

Shalaeva, A.Y.; Kostyuchenko, R.P.; Kozin, V.V. Structural and Functional Characterization of the FGF Signaling Pathway in Regeneration of the Polychaete Worm Alitta virens (Annelida, Errantia). Genes 2021, 12, 788. https://doi.org/10.3390/genes12060788

AMA Style

Shalaeva AY, Kostyuchenko RP, Kozin VV. Structural and Functional Characterization of the FGF Signaling Pathway in Regeneration of the Polychaete Worm Alitta virens (Annelida, Errantia). Genes. 2021; 12(6):788. https://doi.org/10.3390/genes12060788

Chicago/Turabian Style

Shalaeva, Alexandra Y., Roman P. Kostyuchenko, and Vitaly V. Kozin 2021. "Structural and Functional Characterization of the FGF Signaling Pathway in Regeneration of the Polychaete Worm Alitta virens (Annelida, Errantia)" Genes 12, no. 6: 788. https://doi.org/10.3390/genes12060788

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop