Genetic Diversity and Genome-Wide Association Study of Seed Aspect Ratio Using a High-Density SNP Array in Peanut (Arachis hypogaea L.)
Abstract
:1. Introduction
1.1. Peanut Information
1.2. Peanut Germplasms and Core Collection
1.3. Characteristic of Peanut Genome
1.4. Development of Molecular Markers Using Next Generation Sequencing (NGS) Technology
1.5. Applications of High-Density SNP Arrays in Crops
1.6. Purpose
2. Materials and Methods
2.1. Plant Materials, DNA Extraction, and Genotyping
2.2. Screening of Seed Aspect Ratio
2.3. Population Structure Analysis
2.4. Genome-Wide Association Analysis
2.5. Linkage Disequilibrium (LD) Analysis
2.6. Regularization Method
3. Results
3.1. SNP Genotyping
3.2. Phenotype Data Analysis
3.3. Genetic Diversity
3.4. Genetic Structure
3.5. Genome-Wide Association Study (GWAS)
3.6. LD and Candidate Genes Analysis
3.7. Regularization Method
3.8. Evaluation of Heterozygous Rate
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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SNP | Chromosome | Position (bp) | p-Value (p) | FDR_Adjusted_p-Values |
---|---|---|---|---|
AX-177640219 | Araip.B08 | 12829161 | 2.31 × 10−6 | 0.032 |
AX-147235444 | Aradu.A10 | 8911644 | 5.91 × 10−5 | NS a |
AX-176807953 | Aradu.A09 | 113907685 | 6.95 × 10−5 | NS |
AX-176822392 | Araip.B08 | 121783058 | 9.55 × 10−5 | NS |
AX-147262340 | Araip.B09 | 143554366 | 9.80 × 10−5 | NS |
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Zou, K.; Kim, K.-S.; Kim, K.; Kang, D.; Park, Y.-H.; Sun, H.; Ha, B.-K.; Ha, J.; Jun, T.-H. Genetic Diversity and Genome-Wide Association Study of Seed Aspect Ratio Using a High-Density SNP Array in Peanut (Arachis hypogaea L.). Genes 2021, 12, 2. https://doi.org/10.3390/genes12010002
Zou K, Kim K-S, Kim K, Kang D, Park Y-H, Sun H, Ha B-K, Ha J, Jun T-H. Genetic Diversity and Genome-Wide Association Study of Seed Aspect Ratio Using a High-Density SNP Array in Peanut (Arachis hypogaea L.). Genes. 2021; 12(1):2. https://doi.org/10.3390/genes12010002
Chicago/Turabian StyleZou, Kunyan, Ki-Seung Kim, Kipoong Kim, Dongwoo Kang, Yu-Hyeon Park, Hokeun Sun, Bo-Keun Ha, Jungmin Ha, and Tae-Hwan Jun. 2021. "Genetic Diversity and Genome-Wide Association Study of Seed Aspect Ratio Using a High-Density SNP Array in Peanut (Arachis hypogaea L.)" Genes 12, no. 1: 2. https://doi.org/10.3390/genes12010002