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Open AccessArticle

Genome-Wide Identification and Expression Profiling of Monosaccharide Transporter Genes Associated with High Harvest Index Values in Rapeseed (Brassica napus L.)

by Liyuan Zhang 1,2, Chao Zhang 1,2, Bo Yang 1,2, Zhongchun Xiao 1,2, Jinqi Ma 1,2, Jingsen Liu 1,2, Hongju Jian 1,2, Cunmin Qu 1,2, Kun Lu 3 and Jiana Li 1,2,*
1
Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
2
Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
3
State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Beibei, Chongqing 400715, China
*
Author to whom correspondence should be addressed.
Genes 2020, 11(6), 653; https://doi.org/10.3390/genes11060653
Received: 15 May 2020 / Revised: 10 June 2020 / Accepted: 12 June 2020 / Published: 15 June 2020
(This article belongs to the Section Plant Genetics and Genomics)
Sugars are important throughout a plant’s lifecycle. Monosaccharide transporters (MST) are essential sugar transporters that have been identified in many plants, but little is known about the evolution or functions of MST genes in rapeseed (Brassica napus). In this study, we identified 175 MST genes in B. napus, 87 in Brassica oleracea, and 83 in Brassica rapa. These genes were separated into the sugar transport protein (STP), polyol transporter (PLT), vacuolar glucose transporter (VGT), tonoplast monosaccharide transporter (TMT), inositol transporter (INT), plastidic glucose transporter (pGlcT), and ERD6-like subfamilies, respectively. Phylogenetic and syntenic analysis indicated that gene redundancy and gene elimination have commonly occurred in Brassica species during polyploidization. Changes in exon-intron structures during evolution likely resulted in the differences in coding regions, expression patterns, and functions seen among BnMST genes. In total, 31 differentially expressed genes (DEGs) were identified through RNA-seq among materials with high and low harvest index (HI) values, which were divided into two categories based on the qRT-PCR results, expressed more highly in source or sink organs. We finally identified four genes, including BnSTP5, BnSTP13, BnPLT5, and BnERD6-like14, which might be involved in monosaccharide uptake or unloading and further affect the HI of rapeseed. These findings provide fundamental information about MST genes in Brassica and reveal the importance of BnMST genes to high HI in B. napus. View Full-Text
Keywords: monosaccharide transporter; phylogenetic analysis; expression pattern; harvest index; Brassica napus monosaccharide transporter; phylogenetic analysis; expression pattern; harvest index; Brassica napus
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MDPI and ACS Style

Zhang, L.; Zhang, C.; Yang, B.; Xiao, Z.; Ma, J.; Liu, J.; Jian, H.; Qu, C.; Lu, K.; Li, J. Genome-Wide Identification and Expression Profiling of Monosaccharide Transporter Genes Associated with High Harvest Index Values in Rapeseed (Brassica napus L.). Genes 2020, 11, 653.

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