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Intestinal Transcriptome Analysis Highlights Key Differentially Expressed Genes Involved in Nutrient Metabolism and Digestion in Yellowtail Kingfish (Seriola lalandi) Fed Terrestrial Animal and Plant Proteins

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Genecology Research Centre and School of Science and Engineering, University of the Sunshine Coast, Maroochydore, DC 4558, Australia
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Research Institute for Aquaculture No.1, Dinh Bang, Tu Son 220000, Bac Ninh, Vietnam
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New South Wales Department of Primary Industries, Port Stephens Fisheries Institute, Taylors Beach, New South Wales 2316, Australia
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College of Science and Engineering & Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Townsville 4801, Australia
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Ridley Aquafeed Pty Ltd., 4/31 Robart Court, Narangba, Queensland 4504, Australia
*
Author to whom correspondence should be addressed.
Genes 2020, 11(6), 621; https://doi.org/10.3390/genes11060621
Received: 14 May 2020 / Revised: 28 May 2020 / Accepted: 2 June 2020 / Published: 5 June 2020
(This article belongs to the Section Animal Genetics and Genomics)
This study investigated the effects of dietary terrestrial animal and plant proteins on the intestinal transcriptomes of yellowtail kingfish (YTK), Seriola lalandi, an ecologically and economically important marine species in Australia. Five diets containing fish meal (FM), poultry by-product meal (PBM), blood meal (BLM), faba bean meal (FBM) and corn gluten meal (CGM) were formulated and fed over a period of 4 weeks. The Illumina RNA-sequencing (RNA-Seq) results identified a suite of differentially expressed genes involved in nutrient metabolism and protein digestion pathways, reinforced by quantitative polymerase chain reaction (qPCR) results. These findings provide molecular support to the notion that PBM and FBM are useful raw materials in commercial diets for YTK. Using the same evidence, we have demonstrated that BLM and CGM may be less useful and their incorporation into commercial aquafeeds for this species should be done cautiously. The differentially expressed genes showed a subtle difference and high correlation with apparent nutrient digestibility of raw materials. Further, our results indicate that transcriptome profiling provides a useful tool to evaluate alternative protein sources for use in aquaculture feeds. View Full-Text
Keywords: yellowtail kingfish; RNA-sequencing; transcriptomes; alternative protein sources; distal intestine; digestion yellowtail kingfish; RNA-sequencing; transcriptomes; alternative protein sources; distal intestine; digestion
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MDPI and ACS Style

Dam, C.T.M.; Ventura, T.; Booth, M.; Pirozzi, I.; Salini, M.; Smullen, R.; Elizur, A. Intestinal Transcriptome Analysis Highlights Key Differentially Expressed Genes Involved in Nutrient Metabolism and Digestion in Yellowtail Kingfish (Seriola lalandi) Fed Terrestrial Animal and Plant Proteins. Genes 2020, 11, 621. https://doi.org/10.3390/genes11060621

AMA Style

Dam CTM, Ventura T, Booth M, Pirozzi I, Salini M, Smullen R, Elizur A. Intestinal Transcriptome Analysis Highlights Key Differentially Expressed Genes Involved in Nutrient Metabolism and Digestion in Yellowtail Kingfish (Seriola lalandi) Fed Terrestrial Animal and Plant Proteins. Genes. 2020; 11(6):621. https://doi.org/10.3390/genes11060621

Chicago/Turabian Style

Dam, Chinh T.M., Tomer Ventura, Mark Booth, Igor Pirozzi, Michael Salini, Richard Smullen, and Abigail Elizur. 2020. "Intestinal Transcriptome Analysis Highlights Key Differentially Expressed Genes Involved in Nutrient Metabolism and Digestion in Yellowtail Kingfish (Seriola lalandi) Fed Terrestrial Animal and Plant Proteins" Genes 11, no. 6: 621. https://doi.org/10.3390/genes11060621

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