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Article

Characterization of POU2F1 Gene and Its Potential Impact on the Expression of Genes Involved in Fur Color Formation in Rex Rabbit

1
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
2
Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China
*
Author to whom correspondence should be addressed.
Genes 2020, 11(5), 575; https://doi.org/10.3390/genes11050575
Received: 26 April 2020 / Revised: 15 May 2020 / Accepted: 18 May 2020 / Published: 20 May 2020
(This article belongs to the Special Issue Coat Color Genetics)
The naturally colorful fur of the Rex rabbit is becoming increasingly popular in the modern textile market. Our previous study found that POU class 2 homeobox 1 gene (POU2F1) potentially affects the expression of genes involved in fur color formation in the Rex rabbit, but the function and regulation of POU2F1 has not been reported. In this study, the expression patterns of POU2F1 in Rex rabbits of various colors, as well as in different organs, were analyzed by RT-qPCR. Interference and overexpression of POU2F1 were used to identify the potential effects of POU2F1 on other genes related to fur color formation. The results show that the levels of POU2F1 expression were significantly higher in the dorsal skin of the brown and protein yellow Rex rabbits, compared with that of the black one. POU2F1 mRNAs were widespread in the tissues examined in this study and showed the highest level in the lungs. By transfecting rabbit melanocytes with an POU2F1-overexpression plasmid, we found that the POU2F1 protein was located at the nucleus, and the protein showed the classic characteristics of a transcription factor. In addition, abnormal expression of POU2F1 significantly affected the expression of pigmentation-related genes, including SLC7A11, MITF, SLC24A5, MC1R, and ASIP, revealing the regulatory roles of POU2F1 on pigmentation. The results provide the basis for further exploration of the role of POU2F1 in fur color formation of the Rex rabbit. View Full-Text
Keywords: POU2F1; fur color; Rex rabbit; overexpression; RNAi POU2F1; fur color; Rex rabbit; overexpression; RNAi
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MDPI and ACS Style

Yang, N.; Zhao, B.; Hu, S.; Bao, Z.; Liu, M.; Chen, Y.; Wu, X. Characterization of POU2F1 Gene and Its Potential Impact on the Expression of Genes Involved in Fur Color Formation in Rex Rabbit. Genes 2020, 11, 575. https://doi.org/10.3390/genes11050575

AMA Style

Yang N, Zhao B, Hu S, Bao Z, Liu M, Chen Y, Wu X. Characterization of POU2F1 Gene and Its Potential Impact on the Expression of Genes Involved in Fur Color Formation in Rex Rabbit. Genes. 2020; 11(5):575. https://doi.org/10.3390/genes11050575

Chicago/Turabian Style

Yang, Naisu, Bohao Zhao, Shuaishuai Hu, Zhiyuan Bao, Ming Liu, Yang Chen, and Xinsheng Wu. 2020. "Characterization of POU2F1 Gene and Its Potential Impact on the Expression of Genes Involved in Fur Color Formation in Rex Rabbit" Genes 11, no. 5: 575. https://doi.org/10.3390/genes11050575

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