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Volume 11
Issue 3
10.3390/genes11030281
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Article

Mapping-by-Sequencing via MutMap Identifies a Mutation in ZmCLE7 Underlying Fasciation in a Newly Developed EMS Mutant Population in an Elite Tropical Maize Inbred

by
Quan Hong Tran
1,†,
Ngoc Hong Bui
1,†,
Christian Kappel
2,
Nga Thi Ngoc Dau
1,
Loan Thi Nguyen
1,
Thuy Thi Tran
1,
Tran Dang Khanh
1,3,
Khuat Huu Trung
1,
Michael Lenhard
2 and
Son Lang Vi
1,*
1
Department of Genetic Engineering, The Agricultural Genetics Institute, Km2 Pham Van Dong Street, Hanoi, Vietnam
2
Institute for Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Straße 24–25, House 26, 14476 Potsdam-Golm, Germany
3
Center for Expert, Vietnam National University of Agriculture, Trau Quy, Gia Lam, Hanoi, Vietnam
*
Author to whom correspondence should be addressed.
†
These authors contributed equally.
Genes 2020, 11(3), 281; https://doi.org/10.3390/genes11030281
Received: 14 February 2020 / Revised: 3 March 2020 / Accepted: 3 March 2020 / Published: 6 March 2020
(This article belongs to the Special Issue Recent Advances in Genetics and Breeding of Major Staple Food Crops)
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Abstract

Induced point mutations are important genetic resources for their ability to create hypo- and hypermorphic alleles that are useful for understanding gene functions and breeding. However, such mutant populations have only been developed for a few temperate maize varieties, mainly B73 and W22, yet no tropical maize inbred lines have been mutagenized and made available to the public to date. We developed a novel Ethyl Methanesulfonate (EMS) induced mutation resource in maize comprising 2050 independent M2 mutant families in the elite tropical maize inbred ML10. By phenotypic screening, we showed that this population is of comparable quality with other mutagenized populations in maize. To illustrate the usefulness of this population for gene discovery, we performed rapid mapping-by-sequencing to clone a fasciated-ear mutant and identify a causal promoter deletion in ZmCLE7 (CLE7). Our mapping procedure does not require crossing to an unrelated parent, thus is suitable for mapping subtle traits and ones affected by heterosis. This first EMS population in tropical maize is expected to be very useful for the maize research community. Also, the EMS mutagenesis and rapid mapping-by-sequencing pipeline described here illustrate the power of performing forward genetics in diverse maize germplasms of choice, which can lead to novel gene discovery due to divergent genetic backgrounds. View Full-Text
Keywords: EMS; MutMap; mutagenesis; CLE7; tropical maize; fasciation; mapping EMS; MutMap; mutagenesis; CLE7; tropical maize; fasciation; mapping
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MDPI and ACS Style

Tran, Q.H.; Bui, N.H.; Kappel, C.; Dau, N.T.N.; Nguyen, L.T.; Tran, T.T.; Khanh, T.D.; Trung, K.H.; Lenhard, M.; Vi, S.L. Mapping-by-Sequencing via MutMap Identifies a Mutation in ZmCLE7 Underlying Fasciation in a Newly Developed EMS Mutant Population in an Elite Tropical Maize Inbred. Genes 2020, 11, 281. https://doi.org/10.3390/genes11030281

AMA Style

Tran QH, Bui NH, Kappel C, Dau NTN, Nguyen LT, Tran TT, Khanh TD, Trung KH, Lenhard M, Vi SL. Mapping-by-Sequencing via MutMap Identifies a Mutation in ZmCLE7 Underlying Fasciation in a Newly Developed EMS Mutant Population in an Elite Tropical Maize Inbred. Genes. 2020; 11(3):281. https://doi.org/10.3390/genes11030281

Chicago/Turabian Style

Tran, Quan H., Ngoc H. Bui, Christian Kappel, Nga T.N. Dau, Loan T. Nguyen, Thuy T. Tran, Tran D. Khanh, Khuat H. Trung, Michael Lenhard, and Son L. Vi. 2020. "Mapping-by-Sequencing via MutMap Identifies a Mutation in ZmCLE7 Underlying Fasciation in a Newly Developed EMS Mutant Population in an Elite Tropical Maize Inbred" Genes 11, no. 3: 281. https://doi.org/10.3390/genes11030281

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