Next Article in Journal
Cascade Testing for Fragile X Syndrome in a Rural Setting in Cameroon (Sub-Saharan Africa)
Next Article in Special Issue
Repetitive DNA Restructuring Across Multiple Nicotiana Allopolyploidisation Events Shows a Lack of Strong Cytoplasmic Bias in Influencing Repeat Turnover
Previous Article in Journal
Novel Mutations Found in Individuals with Adult-Onset Pompe Disease
Previous Article in Special Issue
Nuclear–Cytoplasmic Coevolution Analysis of RuBisCO in Synthesized Cucumis Allopolyploid
Open AccessArticle

Robust Cytonuclear Coordination of Transcription in Nascent Arabidopsis thaliana Autopolyploids

1
Department of Biology, Reed College, Portland, OR 97202, USA
2
School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA
*
Author to whom correspondence should be addressed.
Genes 2020, 11(2), 134; https://doi.org/10.3390/genes11020134
Received: 1 January 2020 / Revised: 17 January 2020 / Accepted: 24 January 2020 / Published: 28 January 2020
(This article belongs to the Special Issue Cytonuclear Interactions in Polyploid Species)
Polyploidy is hypothesized to cause dosage imbalances between the nucleus and the other genome-containing organelles (mitochondria and plastids), but the evidence for this is limited. We performed RNA-seq on Arabidopsis thaliana diploids and their derived autopolyploids to quantify the degree of inter-genome coordination of transcriptional responses to nuclear whole genome duplication in two different organs (sepals and rosette leaves). We show that nuclear and organellar genomes exhibit highly coordinated responses in both organs. First, organelle genome copy number increased in response to nuclear whole genome duplication (WGD), at least partially compensating for altered nuclear genome dosage. Second, transcriptional output of the different cellular compartments is tuned to maintain diploid-like levels of relative expression among interacting genes. In particular, plastid genes and nuclear genes whose products are plastid-targeted show coordinated down-regulation, such that their expression levels relative to each other remain constant across ploidy levels. Conversely, mitochondrial genes and nuclear genes with mitochondrial targeting show either constant or coordinated up-regulation of expression relative to other nuclear genes. Thus, cytonuclear coordination is robust to changes in nuclear ploidy level, with diploid-like balance in transcript abundances achieved within three generations after nuclear whole genome duplication.
Keywords: polyploidy; Arabidopsis thaliana; cytonuclear interactions; transcription polyploidy; Arabidopsis thaliana; cytonuclear interactions; transcription
MDPI and ACS Style

Coate, J.E.; Schreyer, W.M.; Kum, D.; Doyle, J.J. Robust Cytonuclear Coordination of Transcription in Nascent Arabidopsis thaliana Autopolyploids. Genes 2020, 11, 134.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop