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Open AccessArticle

Identification of Caprine KRTAP28-1 and Its Effect on Cashmere Fiber Diameter

by Jiqing Wang 1,2, Huitong Zhou 1,2,3, Jon G. H. Hickford 1,2,3, Mengli Zhao 1,2, Hua Gong 1,2,3, Zhiyun Hao 1,2, Jiyuan Shen 1,2, Jiang Hu 1,2, Xiu Liu 1,2, Shaobin Li 1,2 and Yuzhu Luo 1,2,*
1
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
2
International Wool Research Institute, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
3
Gene-Marker Laboratory, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
*
Author to whom correspondence should be addressed.
Genes 2020, 11(2), 121; https://doi.org/10.3390/genes11020121
Received: 10 December 2019 / Revised: 20 January 2020 / Accepted: 21 January 2020 / Published: 22 January 2020
The keratin-associated proteins (KAPs) are constituents of cashmere fibers and variation in many KAP genes (KRTAPs) has been found to be associated with fiber traits. The gene encoding the high-sulphur KAP28-1 has been described in sheep, but it has not been identified in the goat genome. In this study, a 255-bp open reading frame on goat chromosome 1 was identified using a search of similar sequence to ovine KRTAP28-1, and that would if transcribed and translated encode a high sulphur KAP. Based on the analysis of polymerase chain reaction amplicons for the goat nucleotide sequences in 385 Longdong cashmere goats in China, five unique banding patterns were detected using single strand conformation polymorphism analysis. These represented five DNA sequences (named variants A to E) and they had the highest resemblance to KRTAP28-1 sequences from sheep, suggesting AE are variants of caprine KRTAP28-1. DNA sequencing revealed a 2 or 4-bp deletion and eleven nucleotide sequence differences, including four non-synonymous substitutions. Of the four common variants (A, B, C and D) found in these goats, the presence of variant A was associated with decreased mean fiber diameter and this effect appeared to be additive. These results indicate that caprine KRTAP28-1 variation might have value as a molecular marker for reducing cashmere mean fiber diameter. View Full-Text
Keywords: goat; keratin-associated protein 28-1 gene (KRTAP28-1); variation; cashmere fiber diameter goat; keratin-associated protein 28-1 gene (KRTAP28-1); variation; cashmere fiber diameter
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Wang, J.; Zhou, H.; Hickford, J.G.H.; Zhao, M.; Gong, H.; Hao, Z.; Shen, J.; Hu, J.; Liu, X.; Li, S.; Luo, Y. Identification of Caprine KRTAP28-1 and Its Effect on Cashmere Fiber Diameter. Genes 2020, 11, 121.

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