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Article

Transcriptional Analysis of Carotenoids Accumulation and Metabolism in a Pink-Fleshed Lemon Mutant

1
Department of Agriculture, Food and Environment, University of Catania, 95123 Catania, Italy
2
Food Biotechnology Department, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Paterna, 46980 Valencia, Spain
*
Author to whom correspondence should be addressed.
Genes 2020, 11(11), 1294; https://doi.org/10.3390/genes11111294
Received: 8 September 2020 / Revised: 20 October 2020 / Accepted: 28 October 2020 / Published: 30 October 2020
(This article belongs to the Special Issue Marker-Trait Association Analysis in Horticultural Crops)
Pink lemon is a spontaneous bud mutation of lemon (Citrus limon, L. Burm. f) characterized by the production of pink-fleshed fruits due to an unusual accumulation of lycopene. To elucidate the genetic determinism of the altered pigmentation, comparative carotenoid profiling and transcriptional analysis of both the genes involved in carotenoid precursors and metabolism, and the proteins related to carotenoid-sequestering structures were performed in pink-fleshed lemon and its wild-type. The carotenoid profile of pink lemon pulp is characterized by an increased accumulation of linear carotenoids, such as lycopene, phytoene and phytofluene, from the early stages of development, reaching their maximum in mature green fruits. The distinctive phenotype of pink lemon is associated with an up-regulation and down-regulation of the genes upstream and downstream the lycopene cyclase, respectively. In particular, 9-cis epoxycarotenoid dioxygenase genes were overexpressed in pink lemon compared with the wild-type, suggesting an altered regulation of abscisic acid biosynthesis. Similarly, during early development of the fruits, genes of the carotenoid-associated proteins heat shock protein 21, fibrillin 1 and 2 and orange gene were overexpressed in the pulp of the pink-fleshed lemon compared to the wild-type, indicating its increased capacity for sequestration, stabilization or accumulation of carotenes. Altogether, the results highlighted significant differences at the transcriptomic level between the pink-fleshed lemon and its wild-type, in terms of carotenoid metabolism and the capacity of stabilization in storage structures between the two accessions. Such changes may be either responsible for the altered carotenoid accumulation or in contrast, a metabolic consequence. View Full-Text
Keywords: carotenoids; citrus; gene expression; fruit quality; Citrus limon; lycopene; pigments carotenoids; citrus; gene expression; fruit quality; Citrus limon; lycopene; pigments
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MDPI and ACS Style

Lana, G.; Zacarias-Garcia, J.; Distefano, G.; Gentile, A.; Rodrigo, M.J.; Zacarias, L. Transcriptional Analysis of Carotenoids Accumulation and Metabolism in a Pink-Fleshed Lemon Mutant. Genes 2020, 11, 1294. https://doi.org/10.3390/genes11111294

AMA Style

Lana G, Zacarias-Garcia J, Distefano G, Gentile A, Rodrigo MJ, Zacarias L. Transcriptional Analysis of Carotenoids Accumulation and Metabolism in a Pink-Fleshed Lemon Mutant. Genes. 2020; 11(11):1294. https://doi.org/10.3390/genes11111294

Chicago/Turabian Style

Lana, Giuseppe, Jaime Zacarias-Garcia, Gaetano Distefano, Alessandra Gentile, María J. Rodrigo, and Lorenzo Zacarias. 2020. "Transcriptional Analysis of Carotenoids Accumulation and Metabolism in a Pink-Fleshed Lemon Mutant" Genes 11, no. 11: 1294. https://doi.org/10.3390/genes11111294

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